和厚朴酚对白菜软腐病菌的抑菌活性及抑菌机理初探

doi:10.11924/j.issn.1000-6850.casb2025-0325

摘要/Abstract

摘要：

本研究旨在探究和厚朴酚对白菜软腐病菌(Pcc)的抑菌效果和抑菌机理。本试验以Pcc为供试菌株，和厚朴酚为供试化合物，通过抑菌圈法及浊度法测定和厚朴酚对Pcc的抑菌效果；通过细胞壁完整性检测、细胞膜完整性和通透性检测及呼吸作用检测探究其抑菌机理。抑菌实验结果表明，10 mg/mL和厚朴酚抑菌圈直径为23.71±0.86 mm，其最低抑菌浓度为0.0156 mg/mL。和厚朴酚作用后使Pcc胞外AKP活力、PI荧光强度、胞外核酸泄漏量和蛋白质泄漏量显著(P<0.05)升高，使细胞膜电位和总ATP酶活力显著(P<0.05)降低。以上实验结果表明，和厚朴酚对Pcc具有显著抑菌活性，其通过破坏细胞壁与细胞膜结构完整性、提高细胞膜通透性及抑制Pcc呼吸作用实现抑菌作用。

关键词: 植物源杀菌剂, 和厚朴酚, 白菜软腐病菌, 抑菌活性, 抑菌机理

Abstract:

This study aimed to investigate the antibacterial activity and mechanism of honokiol against Pectobacterium carotovorum subsp. carotovorum (Pcc). This experiment used Pcc as the test strain and honokiol as the test compound, to determine the antibacterial effect of honokiol against Pcc using the inhibition zone method and turbidimetric method, and explored its antibacterial mechanism by testing cell wall integrity, cell membrane integrity and permeability, and respiratory activity. The results of the antibacterial experiment showed that the inhibition zone diameter of 10 mg/mL honokiol was 23.71±0.86 mm, and its minimum inhibitory concentration value was 0.0156 mg/mL. After honokiol treatment, extracellular alkaline phosphatase (AKP) activity, propidium iodide (PI) fluorescence intensity, extracellular nucleic acid leakage, and protein leakage increased significantly (P<0.05), whereas cell membrane potential and total ATPase (T-ATPase) activity decreased significantly (P<0.05).These results indicated that honokiol exhibited significant antibacterial activity against Pcc by disrupting cell wall and membrane structures, increasing membrane permeability, and inhibiting respiratory function.

Key words: plant-derived fungicide, honokiol, soft rot of Chinese cabbage, antibacterial activity, antibacterial mechanism

王佳飞, 吕航君, 解佳乐, 蔡瑾. 和厚朴酚对白菜软腐病菌的抑菌活性及抑菌机理初探[J]. 中国农学通报, 2025, 41(29): 99-106.

WANG Jiafei, LYU Hangjun, XIE Jiale, CAI Jin. Inhibitory Activity and Mechanism of Honokiol Against Chinese Cabbage Soft Rot[J]. Chinese Agricultural Science Bulletin, 2025, 41(29): 99-106.

图/表 9

图1. 和厚朴酚对Pcc抑菌效果图

图2. 和厚朴酚对白菜软腐病菌抑菌圈直径的测定结果采用Duncan新复极差法分析实验数据的显著性，0.05水平上的显著差异以小写字母表示，下同

杀菌剂	浓度/(mg/mL)										3%DMSO
杀菌剂	2.0000	1.0000	0.5000	0.2500	0.1250	0.0625	0.0312	0.0156	0.0078	0.0039	3%DMSO
和厚朴酚	-	-	-	-	-	-	-	-	+	+++	+++
噻唑锌	-	-	-	-	-	+	+++	+++	+++	+++	+++

表1. 和厚朴酚对Pcc的最低抑菌浓度

图3. 和厚朴酚对Pcc胞外AKP活性的影响

图4. 和厚朴酚对Pcc细胞膜的影响

图5. 和厚朴酚对Pcc膜电位的影响

图6. 和厚朴酚对Pcc核酸泄漏的影响

图7. 和厚朴酚对Pcc蛋白质泄漏的影响

图8. 和厚朴酚对Pcc总ATP酶(T-ATPase)活性的影响

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