不同花生品种苗期耐盐性比较及生理响应机制解析

doi:10.11924/j.issn.1000-6850.casb2025-0713

中国农学通报 ›› 2026, Vol. 42 ›› Issue (14): 46-56.doi: 10.11924/j.issn.1000-6850.casb2025-0713

不同花生品种苗期耐盐性比较及生理响应机制解析

邵梦瑶(), 孔祥军, 贾佩佩, 李恒彬, 闫思慧, 孙辰昕, 李丽杰, 李增强(), 张志勇

河南科技学院生命科学学院，河南新乡 453003

收稿日期:2026-01-12 修回日期:2026-04-28 出版日期:2026-07-25 发布日期:2026-07-24
通讯作者:
李增强，男，1990年出生，河南林州人，讲师，博士，研究方向：作物遗传育种与逆境分子生物学。通信地址：453003 河南省新乡市红旗区华兰大道655号河南科技学院，E-mail：lizengqiang2020@163.com。
作者简介:
邵梦瑶，女，2001年出生，河南汝州人，在读硕士，研究方向：作物逆境分子生物学。通信地址：453003 河南省新乡市红旗区华兰大道655号河南科技学院，E-mail：shaomengyao2001@163.com。
基金资助:
中原科技创新领军人才项目“褪黑素调控棉花和花生氮效率的miRNAs机制与应用”(214200510021); 河南省科技研发计划联合基金（学科类）“NADPH氧化酶基因负调控花生耐盐性的转录机制研究”(252301420129); 河南省科技攻关项目“NADPH氧化酶基因AhRbohB6调控花生耐盐性的功能解析及耐盐新种质创制”(262102111132)

Comparison of Salt Tolerance at Seedling Stage and Analysis of Physiological Response Mechanism in Different Peanut Varieties

SHAO Mengyao(), KONG Xiangjun, JIA Peipei, LI Hengbin, YAN Sihui, SUN Chenxin, LI Lijie, LI Zengqiang(), ZHANG Zhiyong

School of Life Sciences, Henan Institute of Science and Technology, Xinxiang, Henan 453003

Received:2026-01-12 Revised:2026-04-28 Published:2026-07-25 Online:2026-07-24

摘要/Abstract

摘要：

为比较8个‘新百花’系列花生品种在盐胁迫下的耐受能力，并揭示其内在的生理响应机制，本研究采用水培法，在300 mmol/L NaCl胁迫条件下，对各品种进行了为期7天的处理。通过测定各品种的株高、生物量（鲜重和干重）及根系形态等关键生长指标，并利用隶属函数法综合评价其耐盐性。基于结果，选取耐盐性差异最大的4个品种，分析其叶片叶绿素含量和活性氧(ROS)含量，以及抗氧化酶活性等生理指标。结果表明：盐胁迫均显著抑制了各品种的株高、生物量和根系长势，其中‘新百花25’受抑制程度最低，其株高、整株鲜重、整株干重和总根长分别显著降低了14.29%、53.69%、29.64%和43.63%；而‘新百花15’受抑制程度最严重，相应指标分别显著降低了32.19%、68.94%、37.72%和54.82%。结合隶属函数耐盐性综合评价结果表明，‘新百花25’的耐盐性最强，其次是‘新百花27’；‘新百花15’和‘新百花17’则被鉴定为最不耐盐的品种。生理指标分析结果表明，耐盐性强的‘新百花25’和‘新百花27’在盐胁迫下，其叶片中O₂^-、H₂O₂和MDA含量显著降低，叶绿素含量及POD、CAT、APX等抗氧化酶的活性显著升高，表明耐盐性强的花生品种具有更强的抗氧化能力和光合作用能力，从而缓解了盐胁迫对花生生长的抑制。综上，本研究对8个花生品种的耐盐性进行了比较分析，为进一步研究花生响应盐胁迫的分子机制提供了理论依据，更为培育耐盐新品种提供了宝贵的种质资源。

关键词: 花生, 盐胁迫, 耐盐性, 活性氧, 抗氧化酶

Abstract:

To compare the salt tolerance of 8 peanut varieties in Xinbaihua series under salt stress and analyze their physiological response mechanism. In this study, different peanut varieties were treated by hydroponics for 7 days respectively under 300 mmol/L NaCl stress. The plant height, biomass (fresh and dry weight) and root morphological indicators of each variety were measured, and the salt tolerance was comprehensively evaluated by membership function methods. Based on above results, 4 varieties with the greatest differences in salt tolerance were selected, and the physiological indexes, such as leaf chlorophyll content, reactive oxygen species (ROS) content and antioxidant enzyme activity were determined. The results showed that salt stress inhibited the plant height, biomass and root growth of each variety significantly. Among them, the inhibition of ‘Xinbaihua 25’ was the lowest, and its plant height, fresh weight, dry weight and total root length decreased by 14.29%, 53.69%, 29.64% and 43.63%, respectively; while ‘Xinbaihua 15’ was the most severely suppressed, corresponding indicators significantly decreased by 32.19%, 68.94%, 37.72% and 54.82%. The comprehensive evaluation results combined with membership function showed that the salt tolerance of ‘Xinbaihua 25’ was the strongest, followed by ‘Xinbaihua 27’, and the salt tolerance of ‘Xinbaihua 15’ and ‘Xinbaihua 17’ were the worst. The results of physiological indexes showed that compared with ‘Xinbaihua 15’ and ‘Xinbaihua 17’, the contents of O₂^-, H₂O₂ and MDA in leaves of ‘Xinbaihua 25’ and ‘Xinbaihua 27’ with strong salt tolerance were significantly decreased, while the contents of chlorophyll and the activities of POD, CAT and APX were increased. This phenomenon showed that peanut varieties with strong salt tolerance had stronger antioxidant capacity and photosynthetic capacity, thus alleviating the inhibition of salt stress on peanut growth. In short, the salt tolerance of 8 peanut varieties are compared and analyzed systematically, which provide theoretical basis and germplasm resources for further studying the molecular mechanism of their response to salt stress and cultivating salt-tolerant varieties.

Key words: peanut, salt stress, salt tolerance, reactive oxygen species, antioxidant enzyme

中图分类号:

S565.2

邵梦瑶, 孔祥军, 贾佩佩, 李恒彬, 闫思慧, 孙辰昕, 李丽杰, 李增强, 张志勇. 不同花生品种苗期耐盐性比较及生理响应机制解析[J]. 中国农学通报, 2026, 42(14): 46-56.

SHAO Mengyao, KONG Xiangjun, JIA Peipei, LI Hengbin, YAN Sihui, SUN Chenxin, LI Lijie, LI Zengqiang, ZHANG Zhiyong. Comparison of Salt Tolerance at Seedling Stage and Analysis of Physiological Response Mechanism in Different Peanut Varieties[J]. Chinese Agricultural Science Bulletin, 2026, 42(14): 46-56.

图/表 11

参考文献 36

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品种（品系）	处理	总根长/cm	根表面积/cm²	根体积/cm³
新百花13	CK	462.58±78.97cd	118.43±27.49cd	2.43±0.39cde
新百花13	NaCl	243.13±72.93ghi	49.36±13.63f	1.15±0.56ef
新百花15	CK	477.68±99.04c	112.10±49.12cd	3.25±1.24bc
新百花15	NaCl	215.81±24.62hi	53.59±14.41f	0.92±0.21f
新百花16	CK	448.13±88.66cde	141.85±12.14bc	4.26±0.67ab
新百花16	NaCl	239.09±13.95ghi	59.88±3.98f	1.17±0.16ef
新百花17	CK	787.78±67.86a	224.13±24.96a	5.08±1.61a
新百花17	NaCl	223.87±44.11hi	56.67±5.32f	1.15±0.09ef
新百花21	CK	693.74±68.39ab	212.46±13.57a	4.60±1.09a
新百花21	NaCl	366.80±12.16def	46.79±6.90f	1.23±0.32ef
新百花23	CK	599.35±72.90b	153.08±31.43b	2.73±0.91cd
新百花23	NaCl	181.46±17.90hi	100.37±15.42de	1.88±0.36def
新百花25	CK	275.15±5.68fgh	64.65±2.68f	1.38±0.39ef
新百花25	NaCl	155.10±83.49i	49.83±10.64f	1.21±0.12ef
新百花27	CK	650.51±70.76b	170.57±8.73b	3.23±0.73bc
新百花27	NaCl	346.63±40.67efg	70.62±3.59ef	1.36±0.08ef

品种（品系）	处理	总根长/cm	根表面积/cm²	根体积/cm³
新百花13	CK	462.58±78.97cd	118.43±27.49cd	2.43±0.39cde
新百花13	NaCl	243.13±72.93ghi	49.36±13.63f	1.15±0.56ef
新百花15	CK	477.68±99.04c	112.10±49.12cd	3.25±1.24bc
新百花15	NaCl	215.81±24.62hi	53.59±14.41f	0.92±0.21f
新百花16	CK	448.13±88.66cde	141.85±12.14bc	4.26±0.67ab
新百花16	NaCl	239.09±13.95ghi	59.88±3.98f	1.17±0.16ef
新百花17	CK	787.78±67.86a	224.13±24.96a	5.08±1.61a
新百花17	NaCl	223.87±44.11hi	56.67±5.32f	1.15±0.09ef
新百花21	CK	693.74±68.39ab	212.46±13.57a	4.60±1.09a
新百花21	NaCl	366.80±12.16def	46.79±6.90f	1.23±0.32ef
新百花23	CK	599.35±72.90b	153.08±31.43b	2.73±0.91cd
新百花23	NaCl	181.46±17.90hi	100.37±15.42de	1.88±0.36def
新百花25	CK	275.15±5.68fgh	64.65±2.68f	1.38±0.39ef
新百花25	NaCl	155.10±83.49i	49.83±10.64f	1.21±0.12ef
新百花27	CK	650.51±70.76b	170.57±8.73b	3.23±0.73bc
新百花27	NaCl	346.63±40.67efg	70.62±3.59ef	1.36±0.08ef

	株高	鲜重	干重	总根长	根表面积
鲜重	0.785^**
干重	0.713^**	0.892^**
总根长	0.663^**	0.716^**	0.627^**
根表面积	0.630^**	0.695^**	0.569^*	0.923^**
根体积	0.679^**	0.669^**	0.457	0.876^**	0.948^**

	株高	鲜重	干重	总根长	根表面积
鲜重	0.785^**
干重	0.713^**	0.892^**
总根长	0.663^**	0.716^**	0.627^**
根表面积	0.630^**	0.695^**	0.569^*	0.923^**
根体积	0.679^**	0.669^**	0.457	0.876^**	0.948^**

品种（品系）		隶属函数值(μ)							耐盐性综合评价值(D)
品种（品系）		μ₁		μ₂		μ₃	μ₄		值		排名
新百花13	0.40		0.26		0.61			0.74		0.42		6
新百花15	0.00		0.20		0.55			0.32		0.16		8
新百花16	0.47		0.47		0.70			0.35		0.49		5
新百花17	0.40		0.03		0.74			0.33		0.34		7
新百花21	0.46		0.82		0.84			1.00		0.66		3
新百花23	0.84		0.35		0.00			0.56		0.56		4
新百花25	0.64		1.00		0.69			0.00		0.69		1
新百花27	1.00		0.00		1.00			0.45		0.67		2

不同花生品种苗期耐盐性比较及生理响应机制解析

Comparison of Salt Tolerance at Seedling Stage and Analysis of Physiological Response Mechanism in Different Peanut Varieties

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