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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (18): 166-179.doi: 10.11924/j.issn.1000-6850.casb2026-0189

• 水产·渔业 • 上一篇    下一篇

鲤GFI1B和TMOD4基因响应维氏气单胞菌感染的表达特征

丁宁1,2(), 王琦2, 张研2, 姜娜3, 邢薇3, 李铁梁3, 马志宏3, 李炯棠2, 张锦2()   

  1. 1 中国农业科学院研究生院， 北京 100081
    2 中国水产科学研究院生物技术研究中心/农业农村部水生动物基因组学重点实验室/北京市渔业生物技术重点实验室， 北京 100141
    3 北京市农林科学院水产科学研究所， 北京 100068
  • 收稿日期:2026-03-15 修回日期:2026-04-23 出版日期:2026-09-25 发布日期:2026-09-24
  • 通讯作者:
    张锦，女，1993年出生，河南西华人，助理研究员，博士，主要从事鲤抗细菌性疾病遗传机理解析。通信地址：100141 北京市丰台区永定路南青塔150号 中国水产科学研究院，Tel：010-68697512，E-mail：。
  • 作者简介:

    丁宁，女，2001年出生，辽宁鞍山人，硕士研究生，研究方向：鱼类功能基因组学。通信地址：100141 北京市丰台区永定路南青塔150号 中国水产科学研究院，E-mail：。

  • 基金资助:
    国家重点研发计划“主要单胃农业动物和水产生物优异种质资源精准鉴定”(2021YFD1200804); 中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金“鲤多组学数据库构建及在抗菌分子模块鉴定中的应用”(2024A006); “水产生物遗传大数据研究及应用创新团队”(2023TD25)

Expression Patterns of GFI1B and TMOD4 Genes in Response to Aeromonas veronii Infection in Common Carp (Cyprinus carpio)

DING Ning1,2(), WANG Qi2, ZHANG Yan2, JIANG Na3, XING Wei3, LI Tieliang3, MA Zhihong3, LI Jiongtang2, ZHANG Jin2()   

  1. 1 Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081
    2 Biotechnology Research Center, Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Genomes, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Aquatic Biotechnology, Beijing 100141
    3 Fisheries Science Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068
  • Received:2026-03-15 Revised:2026-04-23 Published:2026-09-25 Online:2026-09-24

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摘要：

旨在探究鲤独立生长因子1B（growth factor independent 1B，简称GFI1B）和肌动蛋白丝端部调节蛋白（Tropomodulin，简称TMODs）家族中的TMOD4基因，在维氏气单胞菌（Aeromonas veronii，简称A. veronii）急性和慢性感染过程中的表达特征，以揭示这两个基因通过调控造血细胞生成与免疫细胞运动的分子机制。本研究克隆了鲤GFI1B和TMOD4的全长读码区(CDS)，并对序列进行生物信息学分析；在鲤成体慢性感染15 d和鲤白血球细胞急性感染24 h两模型上分别分析GFI1B和TMOD4基因响应A. veronii感染的表达模式；利用STRING12.0软件，基于斑马鱼同源蛋白，预测GFI1B和TMOD4的蛋白互作网络，并结合表达数据进行联合分析。结果显示，鲤GFI1B基因CDS区长度为762个碱基，编码253个氨基酸。TMOD4基因CDS区长度为1032个碱基，编码343个氨基酸。GFI1B蛋白C端有保守的锌指蛋白结构域。TMOD4蛋白包含Tropomodulin和核糖核酸酶抑制子(Ribonuclease Inhibitor)结构域。序列同源性分析表明，鲤GFI1B与安水金线鲃的序列同源性超过91%，但与其他硬骨鱼类同源性仅为43.92%~65.56%。而TMOD4与硬骨鱼类相似性在80.88%~96.79%。在健康鲤的所有检测组织中，GFI1B和TMOD4基因均有表达，GFI1B在头肾表达量最高，TMOD4在头肾表达量仅次于肌肉和皮肤。STRING预测显示，GFI1B与多个造血转录因子存在互作，TMOD4与肌节结构蛋白及细胞骨架调控因子存在互作。鲤感染A. veronii 15 d后，头肾中GFI1B和TMOD4基因的表达量均显著上调，分别为对照组的6.75倍和5.66倍。鲤白血球细胞急性感染A. veronii 6 h后，GFI1B和TMOD4基因表达量均快速上升，12 h后显著降低，24 h再上升。结果表明，GFI1B和TMOD4基因在鲤的头肾组织和白血球细胞中，对A. veronii 的急慢性感染均呈现高表达特征，提示这两个基因在抵抗A. veronii感染中可能协同发挥重要作用。本研究为深入研究GFI1B和TMOD4基因在鱼类抗菌调控中的作用提供重要基础。

关键词: 鲤, 生长因子非依赖性1B转录抑制因子(GFI1B), 肌动蛋白丝端部调节蛋白4(TMOD4), 维氏气单胞菌, 鲤白血球细胞

Abstract:

The study aims to investigate the expression characteristics of growth factor independent 1B (GFI1B) and Tropomodulin (TMODs) family member TMOD4 gene in common carp (Cyprinus carpio) following acute and chronic infection with Aeromonas veronii (A. veronii), in order to reveal the molecular mechanisms by which these two genes participate in antibacterial infection through the regulation of hematopoietic cell generation and immune cell migration pathways. In this study, the full-length coding regions (CDS) of GFI1B and TMOD4 in common carp were cloned, and their sequences were subjected to bioinformatics analysis. The expression patterns of GFI1B and TMOD4 in response to A. veronii infection were analyzed using two models: chronic infection in carp for 15 days and acute infection in carp leukocyte cell lines for 24 hours. Protein-protein interaction networks of GFI1B and TMOD4 were predicted using STRING12.0 software based on zebrafish homologous proteins, followed by joint analysis incorporating expression data. The GFI1B CDS region spanned 762 bp and encoded a 253-amino acid protein, whereas the TMOD4 CDS comprised 1032 bp and translates into a 343-amino acid protein. The GFI1B protein features a conserved zinc finger domain at its C-terminus, while TMOD4 contains both Tropomodulin and ribonuclease inhibitor domains. The common carp GFI1B shared over 91% identity with Sinocyclocheilus anshuiensis but only 43.92% to 65.56% identity with other teleosts, in contrast to TMOD4, which exhibited 80.88% to 96.79% similarity across teleosts. Both genes were expressed in all tested tissues of healthy carp. GFI1B exhibited the highest expression level in the head kidney, while TMOD4 showed expression levels second only to muscles and skin in the head kidney. STRING prediction revealed that GFI1B interacts with multiple hematopoietic transcription factors, and TMOD4 interacts with myotonic structural proteins and cytoskeletal regulatory factors. After 15 days A. veronii infection, GFI1B and TMOD4 transcripts were significantly upregulated in the head kidney, reaching 6.75-fold and 5.66-fold higher levels compared to controls, respectively. In carp leukocyte cell line, acute infection with A. veronii, the expression levels of GFI1B and TMOD4 genes rapidly increased at 6 hours post-infection, significantly decreased at 12 hours, and rose again at 24 hours. These results demonstrated that after acute and chronic A. veronii infections, GFI1B and TMOD4 exhibited high expression in the head kidney tissue and carp leukocyte cells, highlighting these two genes may synergistically play a crucial role in anti-A. veronii defense. Overall, this work provides a foundational basis for further investigation into the antibacterial regulatory functions of GFI1B and TMOD4 in teleosts.

Key words: Cyprinus carpio, growth factor independent 1B transcriptional repressor (GFI1B), Tropomodulin 4 (TMOD4), Aeromonas veronii (A. veronii), carp leucocyte cell line (CLC)

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