人工合成甘蓝型油菜高频率再生研究初报

摘要/Abstract

摘要： 资源材料保存在作物遗传资源的研究与利用中起到非常重要的作用。该实验以不同来源的人工合成甘蓝型油菜KH106，KH109，KH111D为材料，以花序轴，子房，果柄为外植体研究其再生情况，结果表明，由芥蓝和小白菜人工合成的甘蓝型油菜KH106的再生频率极显著高于由羽衣甘蓝和白菜型油菜人工合成的甘蓝型油菜KH109和KH111；与花序轴和子房为外植体再生频率相比，以果柄为外植体平均再生频率最高，差异达到极显著水平；利用不同预处理培养基处理不同时间，结果显示不经过预处理直接接种到分化培养基上再生频率最高。

关键词: 控释氮肥, 控释氮肥, 莴苣, 肥料利用率, 生长, 产量

Abstract: The preservation of crops materials plays very important role in research and utilization of resources. In this paper, artificial synthesis Brassica napus, named KH106, KH109 and KH111D, has been separately studied regeneration capability of scape, germen and flower stalk as explants. It showed that KH106, which derives from B. albograbra Bailey × B. Parichinensis, had higher regeneration frequency than other two lines KH109 and KH111D, which derive from B.oleracea Var aceaphala × B.campetris. As for different explants, average regeneration frequency of flower stalk was higher with significant level than others. In addition, Some different explants were pretreated with different medium for different periods, the result indicated that regeneration frequency is higher when explants were not pretreated and transited directly on the differentiation medium.

中图分类号:

易家玲,周清元,,崔翠,李加纳,,殷家明,,林呐,. 人工合成甘蓝型油菜高频率再生研究初报[J]. 中国农学通报, 2007, 23(12): 49-49.

Yi Jialing, Zhou Qingyuan,, Cui cui, Li Jiana,, Yin Jiaming,, Lin Na,. The Primal Study on Enhancing Regeneration Frequency of Resynthesized Brassica napus[J]. Chinese Agricultural Science Bulletin, 2007, 23(12): 49-49.

参考文献

[1] 程利华,殷家明,林呐,等.甘蓝型黄籽油菜子叶离体培养初报[J].中国农学通报,2005,21(7):31-33.
[2] 韩德俊,曹莉,袁本威,等.甘蓝型油菜花茎高效再生体系研究[J].中国农学通报, 2005,21(6):86-88.
[3] 何云,万光龙,唐桂香,等.提高甘蓝型油菜下胚轴外植体植株再生的研究[J].浙江大学学报(农业与生命科学版),2006,32(1):46-50.
[4] 胡选萍,王道杰,严静梅.甘蓝型油菜子叶高频率再生体系的筛选研究[J].陕西农业科学,2002(9):3-6.
[5] 黄先群.油菜子叶和下胚轴再生体系及其遗传转化的研究进展[J].西南农业学报,2006,19 (1):152-158.
[6] 李会珍,张志军,周伟军,等.基因型和AgNO3对甘蓝型油菜子叶外植体植株再生的影响[J].中国油料作物学报.2003,25(4):20-22.
[7] 唐桂香,周伟军.AgNO3对甘蓝型油菜子叶外植体植株再生的影响[J].中国油料作物学报,2001.23(3):9-11.
[8] 欧阳丽莹,唐章林,殷家明,等.甘蓝型黄籽油菜下胚轴再生体系的初步研究[J].西南农业大学学报(自然科学版),2004,26(5):525-528.
[9] 潘刚,石淑稳,魏泽兰,等.甘蓝型冬性和半冬性油菜子叶高效快速芽再生体系的建立[J].中国油料作物学报,2004,26(3):1-5.
[10] 祁星华,王轶,罗勤,等.影响甘蓝型油菜下胚轴外植体芽高频率再生的因素[J].北方园艺,2005(1):75-76.
[11] 祁永琼,林良斌,董娜,等.甘蓝型油菜小孢子再生体系的优化研究.云南农业大学学报,2005, 20(1):6-10.
[12] 石淑稳,周永明,王新发.影响油菜子叶外植体不定芽高频率再生的因素[J].西北植物学报,1998,18(4):477-482.
[13] 王景雪,孙毅,崔贵梅,等.在油菜组织培养中激素及基因型对下胚轴分化的影响[J].中国油料作物学报,2000,22(1):11-l3.
[14] 王艳,曾幼玲,张富春,等.新疆甘蓝型油菜下胚轴的组织培养和植株再生研究[J].新疆农业科学,2005,42(1):24-28.
[15] 许本波,张学昆,殷家明,等.培养条件对甘蓝型黄籽油菜下胚轴的再生影响[J].中国油料作物学报,2003,25(4):23-26.