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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (24): 73-77.

• 生物技术科学 • 上一篇    下一篇

北沙参SRAP分子标记体系的建立与优化

齐树杰1,沈 镝2,李 颖1,张钦德3,李庆典1   

  • 收稿日期:2009-08-13 修回日期:2009-09-03 出版日期:2009-12-20 发布日期:2009-12-20

  • Received:2009-08-13 Revised:2009-09-03 Online:2009-12-20 Published:2009-12-20

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摘要：

为北沙参的遗传多样性分析提供一种科学的途径。采用SRAP标记技术，以北沙参基因组DNA为模板，优化了SRAP反应体系的各主要参数。建立了稳定可靠的SRAP-PCR反应体系；20μL反应体系中，DNA的量为80ng、Mg2+ 2.5mmol/L、dNTPs 0.25mmol/L、TaqDNA聚合酶为1U、正反向Primer浓度均为0.1μmol/L。该体系适合北沙参遗传多样性分析、遗传图谱构建等研究。

关键词: 粮草间作, 粮草间作, 坡耕地, 培肥保土, 效益

Abstract:

To develop a sicentific method for Glehnia litteralis genetic diversity analysis. Glehnia litteralis DNA was used to optimize SRAP system’s main parameters. A stability and reliable SRAP-PCR system was established. In the 20μL system, DNA 80 ng, Mg2+ 2.5 mmol/L, dNTP 0.25mmol/L, Taq 1U, Upstream and Downstream primers both for 0.25μmol/L. The reaction system was suitable to study Glehnia litteralis genetic diversity analysis and gene mapping.