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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (1): 32-35.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

有机添加物对卡特兰组织培养的影响

马生健,覃金芳,曾富华   

  • 收稿日期:2009-08-07 修回日期:2009-09-11 出版日期:2010-01-05 发布日期:2010-01-05

The effect of organic compounds on the tissue culture of Cattleya

  • Received:2009-08-07 Revised:2009-09-11 Online:2010-01-05 Published:2010-01-05

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摘要：

以卡特兰（Cattleya labiata）品种MC1原球茎（protocorm-like-body，PLB）为外植体，接种到添加不同浓度（10%、15%、20%）的六种有机添加物（椰乳、椰汁、香蕉泥、马铃薯、水解酪蛋白、苹果汁）的改良VW + 5.0 mg?L-1 6-BA + 0.5 mg?L-1 NAA + 20 g?L-1蔗糖 + 16 g?L-1琼脂的培养基上。结果表明：六种有机添加物对PLB增殖的促进作用由大到小依次为椰乳、马铃薯、椰汁、香蕉泥、苹果汁、水解酪蛋白。15%的椰乳对PLB增殖效果最佳，PLB的增殖率最高，而且PLB的分化率最低。材料的年龄对PLB的增殖也有影响作用，年龄越小，增殖率越高，分化率越低。

关键词: 苹果-苹果汁, 苹果-苹果汁, 安全监管, 机制, 对策

Abstract:

The Cattleya labiata MC1 PLB were inoculated on the reformed VW + 5.0 mg/L 6-BA + 0.5 mg/L NAA + 20 g/L srose + 16 g/L agar medium adding six different organic compounds (Coconut milk、coconut juice、banana mud、Potato、Casein hydrollysate、Apple juice) with different concentrations (10%,15%,20%). The results showed that, the promotion effect of these six organic compounds from large to small successively is, Coconut milk、Potato、Coconut juice、Banana mud、Apple juice、Casein hydrollysate. The 15% Coconut milk was the best to the propagation of Cattleya PLB, with the highest propagation rate and the lowest differentiation rate. The ages of the PLB also affected the propagation of the PLB. The younger the PLB were, the higher the propagation rate was, and the lower the differentiation rate was.