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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (31): 138-142.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

小白菜‘四月慢’与自交系05-51的组培再生比较研究

臧运祥 于丹丹 陈春晖 朱祝军   

  • 收稿日期:2011-08-31 修回日期:2011-10-31 出版日期:2011-12-05 发布日期:2011-12-05
  • 基金资助:

    小白菜吲哚硫苷代谢工程及其抗虫效应研究;外源茉莉酸调控小白菜硫代葡萄糖苷生物合成的分子机制及诱导抗虫性的研究;大白菜抗癌硫苷合成关键基因的克隆及功能鉴定研究

Comparison of Plant Regeneration System between Two Pakchoi Cultivars ‘Siyueman’ and Inbred Line 05-51

  • Received:2011-08-31 Revised:2011-10-31 Online:2011-12-05 Published:2011-12-05

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摘要：

以不同器官为研究对象，对小白菜常规种‘四月慢’与自交系种质材料05-51的组培再生体系进行比较分析，为小白菜的遗传转化和种质创新提供重要的理论支持与技术保障。试验结果表明，以带柄子叶为外植体诱导不定芽效果较好；MS培养基中3 mg/L的6-BA与0.5 mg/L的NAA组合有利于不定芽形成。不同基因型之间的再生频率差异较大，与自交系种质材料05-51相比，常规种四月慢更易再生。

关键词: IPT, IPT, 烟草, 种子萌发, 幼苗生长

Abstract:

In order to facilitate the genetic transformation and germplasm innovation, compare the regeneration system difference between two pakchoi cultivars ‘Siyueman’ and inbred line 05-51 using different tissues. The result indicated that the explants of cotyledons with petioles were more efficient to induce adventitious buds. 3 mg/L 6-BA plus 0.5 mg/L NAA in MS basal medium was the most suitable hormone combination for adventitious buds induction. The regeneration rate was quite different among genotypes. Compared with 05-51, Siyueman was more inductive.