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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (11): 29-35.doi: 10.11924/j.issn.1000-6850.casb2022-0219

• 生物科学 • 上一篇    下一篇

药食兼用植物黄蜀葵全长转录组测序及特性分析

叶广英(), 王再花, 刘海林, 李杰()   

  1. 广东省农业科学院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室，广州 510640
  • 收稿日期:2022-03-30 修回日期:2022-06-15 出版日期:2023-04-15 发布日期:2023-04-10
  • 通讯作者: 李杰，男，1983年出生，湖南常德人，副研究员，博士，主要从事花卉栽培与育种方面的研究。通信地址：510640 广州天河区五山路金颖东一街1号 广东省农业科学院环境园艺研究所，Tel：021-85161185，E-mail：goodlijie666@163.com。
  • 作者简介:

    叶广英，男，1988年出生，山东聊城人，博士，研究方向：花卉栽培与育种。通信地址：510640 广州天河区五山路金颖东一街1号 广东省农业科学院环境园艺研究所，Tel：021-85161185，E-mail：。

  • 基金资助:
    广东省重点领域研发计划项目“康养花卉高效育种技术创新与高功能成分新品种选育”(2022B0202080002); 2022年省级农业科技发展及资源环境保护管理项目“南药产业创新团队岗位专家”(2022KJ148); 科技创新战略专项资金（高水平农科院建设）项目“青年研究员”(R2019PY-QY003)

Medicinal and Edible Abelmoschus manihot: Sequencing and Characteristic Analysis of Full-length Transcriptome

YE Guangying(), WANG Zaihua, LIU Hailin, LI Jie()   

  1. Guangdong Provincial Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
  • Received:2022-03-30 Revised:2022-06-15 Online:2023-04-15 Published:2023-04-10

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摘要：

旨在从转录组水平解析黄蜀葵体内进行的主要生物进程及黄酮醇化合物合成的分子机制。利用高通量测序PacBio Sequel平台，以黄蜀葵的花、茎和叶的混合样品为材料，使用单分子实时测序技术(SMRT)进行全长转录组测序。测序数据质控后共获得53925条高质量转录本，其中53329个基因被公共数据库注释。共16574个基因注释到138条途径上，其中注释最多的途径为代谢途径（8556个），其次为次生代谢物的生物合成途径（4650个）。有10700个黄蜀葵基因发生了可变剪切事件，存在7种可变剪切类型。预测到3287个转录因子，bHLH 转录因子家族的基因最多。检测到5683个SSR位点，且AAG/CCT类型最多。鉴定出黄酮醇合成相关的基因170个，包含10个关键的糖基转移酶基因，且CHS和F3H基因发生了内含子保留型的可变剪切。本研究揭示了黄蜀葵转录组的整体水平及黄酮醇生物合成的相关基因，为后期遗传改良和功能基因挖掘与利用提供了分子基础。

关键词: 黄蜀葵, 全长转录组, 黄酮醇, 转录因子, 可变剪切, SSR

Abstract:

The purpose of this study is to elucidate main biological processes and molecular mechanism of flavonol synthesis in Abelmoschus manihot at the transcriptome level. Using the PacBio Sequel platform of high-throughput sequencing, the mixture of flowers, stems and leaves was taken as material, and the full-length transcriptome sequencing was performed by single-molecule real-time sequencing (SMRT) method. After quality control of the sequencing data, a total of 53925 high-quality transcripts were obtained, among which 53329 genes were annotated by public database. There were 16574 genes annotated in 138 pathways, of which the most annotated pathway was metabolic pathway (8556 genes), followed by biosynthesis pathway of secondary metabolites (4650 genes). In total, 10700 genes underwent alternative splicing events, and seven alternative splicing types were presented. Moreover, 3287 transcription factors were predicted, with the bHLH transcription factor family having the most genes. 5683 SSR loci were detected, and the AAG/CCT type was the most. 170 genes related to flavonol synthesis were identified, including 10 key glycosyltransferase genes, and CHS and F3H genes underwent intron-sparing variable splicing. This study revealed the overall level of transcriptome and flavonol biosynthesis pathway genes of A. manihot, and provided reference for its genetic improvement and functional gene mining and utilization.

Key words: Abelmoschus manihot, full-length transcriptome, flavonol, transcription factors, alternative splicing, SSR