草甘膦概况及微生物降解研究进展

doi:10.11924/j.issn.1000-6850.casb2024-0200

中国农学通报 ›› 2024, Vol. 40 ›› Issue (23): 68-74.doi: 10.11924/j.issn.1000-6850.casb2024-0200

• 资源·环境·生态·土壤·气象 • 上一篇下一篇

草甘膦概况及微生物降解研究进展

葛志坚(), 杨峰山, 李昆, 付海燕, 刘春光()

黑龙江大学生命科学学院/农业微生物技术教育部工程研究中心/黑龙江省寒地生态修复与资源利用重点实验室/黑龙江省普通高校微生物重点实验室，哈尔滨 150080

收稿日期:2024-03-28 修回日期:2024-05-22 出版日期:2024-08-09 发布日期:2024-08-09
通讯作者:
刘春光，女，1982年出生，黑龙江哈尔滨人，高级实验师，博士，主要从事长残效除草剂的微生物降解与土壤修复方面的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号，Tel：0451-86608586，E-mail：2005013@hlju.edu.cn。
作者简介:
葛志坚，女，2000年出生，河北保定人，硕士研究生，主要从事长残效除草剂的微生物降解与土壤修复方面的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号，Tel：0451-86608586，E-mail：2221623@s.hlju.edu.cn。
基金资助:
黑龙江省自然科学基金联合引导项目“粘质沙雷氏菌FLB1原卟啉途径参与氯氟吡啶酯降解的分子机理”(LH2022C074)

Overview of Glyphosate and Research Progress in Microbial Degradation

GE Zhijian(), YANG Fengshan, LI Kun, FU Haiyan, LIU Chunguang()

Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education /Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region /Key Laboratory of Microbiology, College of Heilongjiang Province / School of Life Sciences,Heilongjiang University, Harbin, Heilongjiang 150080

Received:2024-03-28 Revised:2024-05-22 Published:2024-08-09 Online:2024-08-09

摘要/Abstract

摘要：

草甘膦作为一种广泛使用的除草剂，因其高效和低成本的特点在农业生产中得到了广泛应用。然而，这种化学物质的使用也给环境带来了一系列的问题。本研究简述了草甘膦作用机理和毒性等概况，阐明草甘膦对水环境的危害主要在影响水质、水生生物和生态系统等方面，对土壤的危害主要体现在破坏土壤微生物生态、破坏土壤微生物群落的结构和功能、影响土壤酶活性。介绍了生物降解的草甘膦污染土壤修复方式的研究进展，草甘膦污染土壤的修复主要运用微生物降解手段。相比于吸附、光解等非生物降解，微生物降解更环保更高效且更具有前景。最后对草甘膦污染土壤修复的微生物降解方式进行了展望，以期为草甘膦微生物降解技术在草甘膦污染土壤中的应用和改进提供参考。

关键词: 除草剂, 草甘膦, 污染土壤, 微生物降解, 土壤修复

Abstract:

The herbicide glyphosate is widely used in agriculture because of its high efficiency and low cost, but it also poses certain harm. This paper provided a brief introduction to glyphosate toxicity and mechanism. It was clarified that the harm of glyphosate to water environment was mainly in the aspects of affecting water quality, aquatic organisms and ecosystems. The harm to soil was mainly reflected in the destruction of soil microbial ecology, the destruction of soil microbial community structure and function, and the influence of soil enzyme activity. The research progress of bioremediation of glyphosate-contaminated soil was introduced. The bioremediation of glyphosate-contaminated soil was mainly microbial degradation methods. Compared with non-biodegradation such as adsorption and photolysis, microbial degradation was more environmentally friendly, efficient and promising. Finally, the microbial degradation methods of glyphosate contaminated soil remediation were prospected, in order to provide reference for the application and improvement of glyphosate microbial degradation technology in glyphosate contaminated soil.

Key words: herbicide, glyphosate, contaminated soil, microbial degradation, soil remediation

葛志坚, 杨峰山, 李昆, 付海燕, 刘春光. 草甘膦概况及微生物降解研究进展[J]. 中国农学通报, 2024, 40(23): 68-74.

GE Zhijian, YANG Fengshan, LI Kun, FU Haiyan, LIU Chunguang. Overview of Glyphosate and Research Progress in Microbial Degradation[J]. Chinese Agricultural Science Bulletin, 2024, 40(23): 68-74.

图/表 2

参考文献 55

[1]	ZHANG W P, LI J Y, ZHANG Y M, et al. Characterization of a novel glyphosate-degrading bacterial species, Chryseobacterium sp. Y16C, and evaluation of its effects on microbial communities in glyphosate-contaminated soil[J]. Journal of hazardous materials, 2022, 432:128689.
[2]	POWLES S B. Evolved glyphosate-resistant weeds around the world: Lessons to be learnt[J]. Pest management science: Formerly pesticide science, 2008, 64(4):360-365.
[3]	CHEN Y, CHEN W J, HUANG Y, et al. Insights into the microbial degradation and resistance mechanisms of glyphosate[J]. Environmental research, 2022, 215:114153.
[4]	陈虎保, 朱惠香, 陈国海. 草甘膦的作用机理及部位[J]. 林业科技通讯, 1997(1):21-23.
[5]	CRUNP K. The potential effects of recall bias and selection bias on the epidemiological evidence for the carcinogenicity of glyphosate[J]. Risk analysis, 2020, 40(4):696-704. doi: 10.1111/risa.13440 pmid: 31889327
[6]	GUYTON K Z, LOOMIS D, GROSSE Y, et al. Carcinogenicity of tetrachlorvinphos, parathion, malathion, diazinon, and glyphosate[J]. The lancet oncology, 2015, 16(5):490-491.
[7]	PEILLEX C, PELLETIER M. The impact and toxicity of glyphosate and glyphosate-based herbicides on health and immunity[J]. Journal of immunotoxicology, 2020, 17(1):163-174. doi: 10.1080/1547691X.2020.1804492 pmid: 32897110
[8]	SINGH S, KUMAR V, GILL J P K, et al. Herbicide glyphosate: Toxicity and microbial degradation[J]. International journal of environmental research and public health, 2020, 17(20):7519.
[9]	WILLIAMS G M, KROES R, MUNRO I C. Safety evaluation and risk assessment of the herbicide roundup and its active ingredient, glyphosate, for humans[J]. Regulatory toxicology and pharmacology, 2000, 31(2):117-165.
[10]	TSUI M T, CHU L M. Aquatic toxicity of glyphosate-based formulations: Comparison between different organisms and the effects of environmental factors[J]. Chemosphere 2003, 52(7):1189-1197. doi: 10.1016/S0045-6535(03)00306-0 pmid: 12821000
[11]	CO E, CD N, LC A, et al. Acute toxicity of glyphosate based herbicide glycot on juvenile African cat fish clarias gariepinus (burchell 1822)[J]. Journal of fisheries & livestock production, 2017, 5(3):1-4.
[12]	ROY N M, CARNEIRO B, OCHS J. Glyphosate induces neurotoxicity in Zebrafish[J]. Environmental toxicology and pharmacology, 2016, 42:45-54. doi: 10.1016/j.etap.2016.01.003 pmid: 26773362
[13]	马晓洁. 草甘膦对大型溞生长繁殖及抗氧化系统的影响[D]. 新乡: 河南师范大学, 2019:10-42.
[14]	CAVALCANTE D G S M, MARTINEZ C B R, SOFIA S H. Genotoxic effects of roundup ® on the fish Prochiloduslineatus[J]. Mutation research/genetic toxicology and environmental mutagenesis, 2008, 655(1-2):41-46.
[15]	洪宇航. 草甘膦对中华绒螯蟹成蟹主要免疫指标的影响[J]. 江苏农业科学, 2018, 46(12):136-140.
[16]	廖艺钰, 惠吕佳, 严吉祥, 等. 草甘膦农药对斑马鱼的急性毒性和慢性毒性研究[J]. 广州化工, 2020, 48(21):66-68.
[17]	KREUTZ L C, BARCELLOS L J G, SILVA T O, et al. Acute toxicity test of agricultural pesticides on silver catfish (Rhamdiaquelen) fingerlings[J]. Ciênciarural, 2008, 38(4):1050-1055.
[18]	PERIRA H A, HERNANDES P R T, NETTO M S, et al. Adsorbents for glyphosate removal in contaminated waters: A review[J]. Environmental chemistry letters, 2021, 19(2):1525-1543.
[19]	SOARES D, SILVA L, DUARTE S, et al. Glyphosate use, toxicity and occurrence in food[J]. Foods, 2021, 10(11):2785.
[20]	STEMPVOORT D R V, ROY J W, BROWN S J, et al. Residues of the herbicide glyphosate in riparian groundwater in urban catchments[J]. Chemosphere, 2014, 95:455-463. doi: 10.1016/j.chemosphere.2013.09.095 pmid: 24206835
[21]	LYNDSAY S, REZA P. Glyphosate in runoff waters and in the root-zone: Areview[J]. Toxics, 2015, 3(4):462-480.
[22]	LI J, ZHANG W, LIN Z, et al. Emerging strategies for the bioremediation of the phenylurea herbicide diuron[J]. Frontiers in microbiology, 2021, 12:686509.
[23]	YU X M, YU T, YIN G H, et al. Glyphosate biodegradation and potential soil bioremediation by Bacillus subtilis strain Bs-15[J]. Genetics and molecular research, 2015, 14(4):14717-14730.
[24]	ERMAKOVA I T, KISELEVA N I, SHUSHKOVA T, et al. Bioremediation of glyphosate-contaminated soils[J]. Applied microbiology and biotechnology, 2010, 88:585-594. doi: 10.1007/s00253-010-2775-0 pmid: 20676632
[25]	PÉREZ RODRÍGUEZ M, MELO C, JIMÉNEZ E, et al. Glyphosate bioremediation through the sarcosine oxidase pathway mediated by Lysinibacillus sphaericus in soils cultivated with potatoes[J]. Agriculture, 2019, 9(10):217.
[26]	ACOSTA-CORTÉS A G, MARTINEZ-LEDEZMA C, LÓPEZ-CHUKEN U J, et al. Polyphosphate recovery by a native Bacillus cereus strain as a direct effect of glyphosate uptake[J]. The ISME journal, 2019, 13(6):1497-1505.
[27]	KRYUCHKOVA Y V, BURYGIN G L, GOGOLEVA N E, et al. Isolation and characterization of a glyphosate-degrading rhizosphere strain, Enterobacter cloacae K7[J]. Microbiological research, 2014, 169(1):99-105.
[28]	MASSOT F, GKOREZIS P, HAMME J V, et al. Isolation, biochemical and genomic characterization of glyphosate tolerant bacteria to perform microbe-assisted phytoremediation[J]. Frontiers in microbiology, 2021, 11:598507.
[29]	LERBS W, STOCK M, PARTHIER B. Physiological aspects of glyphosate degradation in Alcaligenes spec. strain GL[J]. Archives of microbiology, 1990, 153:146-150.
[30]	OBOJSKA A, TERNAN N G, LEJCZAK B, et al. Organophosphonate utilization by the Thermophile Geobacillus caldoxylosilyticus T20[J]. Applied and environmental microbiology, 2002, 68(4):2081-2084.
[31]	PIPKE R, AMRHEIN N, JACOB G S, et al. Metabolism of glyphosate in an Arthrobacter sp. GLP-1[J]. European journal of biochemistry, 1987, 165:267-273.
[32]	PEALOZA-VAZQUEZ A, MENA G L, HERRERA-ESTRELLA L, et al. Cloning and sequencing of the genes involved in glyphosate utilization by Pseudomonas pseudomallei [J]. Applied and environmental microbiology, 1995, 61(2):538-543.
[33]	BALTHAZOR T M, HALLAS L E. Glyphosate-degrading microorganisms from industrial activated sludge[J]. Applied and environmental microbiology, 1986, 51:432-434. doi: 10.1128/aem.51.2.432-434.1986 pmid: 16346999
[34]	FIRDOU S, IQBA S, ANWA S, et al. Identification and analysis of 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene from glyphosate-resistant Ochrobactrum intermedium Sq20[J]. Pest management science, 2018, 74(5):1184-1196.
[35]	MCAULIFE K S, HALLAL E, KULP C F. Glyphosate degradation by Agrobacterium radiobacter isolated from activated sludge[J]. Journal of industrial microbiology, 1990, 6:219-221.
[36]	JACOB G S, GARBO J R, HALLA L E, et al. Metabolism of glyphosate in Pseudomonas sp. strain LBr[J]. Applied and environmental microbiology, 1988, 54(12):2953-2958.
[37]	QUINN J P, PEDEN J M M, DICK R E. Carbon-phosphorus bond cleavage by gram-positive and gram-negative soil bacteria[J]. Applied microbiology and biotechnology, 1989, 31:283-287.
[38]	FAN J, YANG G, ZHAO H, et al. Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil[J]. Journal of general and applied microbiology, 2012, 58(4):263-271.
[39]	FU G, HEN Y, LI R, et al. Pathway and rate-limiting step of glyphosate degradation by Aspergillus oryzae A-F02[J]. Preparative biochemistry and biotechnology, 2017, 47(8):782-788.
[40]	KRZY’KO-LUPICK T, STROF W, KUB K, et al. The ability of soil-borne fungi to degrade organophosphonate carbon-to-phosphorus bonds[J]. Applied microbiology & biotechnology, 1997, 48(4):549-552.
[41]	KRZYŚKO-ŁUPICKA T, ORLIK A. The use of glyphosate as the sole source of phosphorus or carbon for the selection of soil-borne fungal strains capable to degrade this herbicide[J]. Chemosphere, 1997, 34(12):2601-2605.
[42]	BUJACZ B, WIECZOREK P, KRZYSKO-LUPICKA T, et al. Organophosphonate utilization by the wild-type strain of Penicillium notatum[J]. Applied and environmental microbiology, 1995, 61(8):2905-2910.
[43]	OBOJSKA A, LEJCZAK B, KUBRAK M. Degradation of phosphonates by Streptomycete isolates[J]. Applied microbiology and biotechnology, 1999, 51:872-876.
[44]	SVIRIDOV A V, SHUSHKOVA T V, ZELENKOVA N F, et al. Distribution of glyphosate and methylphosphonate catabolism systems in soil bacteria Ochrobactrum anthropi and Achromobactersp[J]. Applied microbiology and biotechnology, 2012, 93:787-796.
[45]	CHEN Y, JIANG Z, WU D, et al. Development of a novel bio-organic fertilizer for the removal of atrazine in soil[J]. Journal of environmental management, 2019, 233:553-560. doi: S0301-4797(18)31508-1 pmid: 30597348
[46]	CONTRERAS M B C, CAMACHO J V, FERNANDEZ-MORALES F J, et al. Biodegradability improvement and toxicity reduction of soil washing effluents polluted with atrazine by means of electrochemical pre-treatment: Influence of the anode material[J]. Journal of environmental management, 2020, 255:109895.
[47]	MORILLO E, VILLAVERDE J. Advanced technologies for the remediation of pesticide-contaminated soils[J]. Science of the total environment, 2017, 586:576-597.
[48]	OUYANG W, HAO X, TYSKLIND M, et al. Typical pesticides diffuse loading and degradation pattern differences under the impacts of climate and land-use variations[J]. Environment international, 2020, 139:105717.
[49]	QU M, LI H, LI N, et al. Distribution of atrazine and its phytoremediation by submerged macrophytes in lake sediments[J]. Chemosphere, 2017, 168:1515-1522. doi: S0045-6535(16)31710-6 pmid: 27932038
[50]	WANG A, HU X, WAN Y, et al. A nationwide study of the occurrence and distribution of atrazine and its degradates in tap water and groundwater in China: Assessment of human exposure potential[J]. Chemosphere, 2020, 252:126533.
[51]	SVIRIDOV A V, SHUSHKOVA T V, ERMAKOVA I T, et al. Microbial degradation of glyphosate herbicides[J]. Applied biochemistry and microbiology, 2015, 51:188-195.
[52]	BAZOT S, LEBEAU T. Simultaneous mineralization of glyphosate and diuron by a consortium of three bacteria as free-and/or immobilized-cells formulations[J]. Applied microbiology and biotechnology, 2008, 77:1351-1358.
[53]	SHUSHKOVA T, ERMAKOVA I, LEONTIEVSKY A. Glyphosate bioavailability in soil[J]. Biodegradation, 2010, 21:403-410. doi: 10.1007/s10532-009-9310-y pmid: 19902365
[54]	胡浩. 生态修复技术在水环境保护中的应用[J]. 科技资讯, 2023, 21(14):108-111.
[55]	GÓNGORA-ECHEVERRÍA V R, GARCÍA-ESCALANTE R, ROJAS-HERRERA R, et al. Pesticide bioremediation in liquid media using a microbial consortium and bacteria-pure strains isolated from a biomixture used in agricultural areas[J]. Ecotoxicology and environmental safety, 2020, 200:110734.

微生物种类	拉丁文名称	中文名称	参考文献
细菌	Arthrobacter sp. GLP-1	节杆菌GLP-1	[31]
	Alcaligenes sp. GL	产碱菌GL	[29]
	Pseudomonas pseudomallei22	类鼻疽假单胞菌22	[32]
	Flavobacterium sp. GD1	黄杆菌GD1	[33]
	Ochrobactrum intermedium Sq20	中间苍白杆菌Sq20	[34]
	Agrobacterium radiobacterSW9	放射杆状土壤杆菌SW9	[35]
	Achromobacter sp. LW9	无色杆菌LW9	[35]
	Pseudomonas sp. LBr	假单胞菌LBr	[36]
	Pseudomonas sp. 4ASW	假单胞菌4ASW	[37]
	Bacillus megaterium 2BLW	巨大芽胞杆菌2BLW	[37]
	Bacillus cereus CB4	蜡样芽孢杆菌CB4	[38]
真菌	Aspergillus oryzae A-F02	米曲霉A-F02	[39]
	Aspergillus niger	黑曲霉	[40]
	Penicillium IIR	青霉菌IIR	[41]
	Mucor IIIR	毛霉菌III	[41]
	Scopulariopsis sp.	帚霉菌	[40]
	Trichoderma harzianum	哈茨木霉	[40]
	Penicillium notatum	点青霉	[42]
放线菌	Streptomycete isolates	链霉菌	[43]

微生物种类	拉丁文名称	中文名称	参考文献
细菌	Arthrobacter sp. GLP-1	节杆菌GLP-1	[31]
	Alcaligenes sp. GL	产碱菌GL	[29]
	Pseudomonas pseudomallei22	类鼻疽假单胞菌22	[32]
	Flavobacterium sp. GD1	黄杆菌GD1	[33]
	Ochrobactrum intermedium Sq20	中间苍白杆菌Sq20	[34]
	Agrobacterium radiobacterSW9	放射杆状土壤杆菌SW9	[35]
	Achromobacter sp. LW9	无色杆菌LW9	[35]
	Pseudomonas sp. LBr	假单胞菌LBr	[36]
	Pseudomonas sp. 4ASW	假单胞菌4ASW	[37]
	Bacillus megaterium 2BLW	巨大芽胞杆菌2BLW	[37]
	Bacillus cereus CB4	蜡样芽孢杆菌CB4	[38]
真菌	Aspergillus oryzae A-F02	米曲霉A-F02	[39]
	Aspergillus niger	黑曲霉	[40]
	Penicillium IIR	青霉菌IIR	[41]
	Mucor IIIR	毛霉菌III	[41]
	Scopulariopsis sp.	帚霉菌	[40]
	Trichoderma harzianum	哈茨木霉	[40]
	Penicillium notatum	点青霉	[42]
放线菌	Streptomycete isolates	链霉菌	[43]

草甘膦概况及微生物降解研究进展

Overview of Glyphosate and Research Progress in Microbial Degradation

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 55

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	肖和友, 刘聪聪, 刘娜梅, 马冠华, 何祥春, 刘蓉, 张赐喜, 于庆涛, 姚廷山. 邵阳市植烟田杂草种类分析及防控研究[J]. 中国农学通报, 2024, 40(6): 152-156.
[2]	徐霄, 张鑫, 章明奎, 黄巧玲, 尹献远. 不同钝化材料对降低农田镉砷污染效果及安全性评估的研究[J]. 中国农学通报, 2024, 40(17): 43-48.
[3]	覃勇荣, 罗美东, 刘旭辉, 黄秀连, 张治坤. 艾蒿自然修复砷等重金属复合污染土壤的效果分析[J]. 中国农学通报, 2024, 40(11): 55-67.
[4]	郭文磊, 张纯, 张泰劼, 田兴山. 草甘膦抗性与敏感牛筋草生物型对干旱胁迫的生理响应[J]. 中国农学通报, 2023, 39(29): 123-128.
[5]	杜东霞, 李咏梅, 喻孟元, 王震, 许丽娟, 吴民熙. 耐镉根际促生菌WYN5的分子鉴定及其对黑麦草富集镉的影响[J]. 中国农学通报, 2023, 39(27): 59-66.
[6]	连磊, 王恒智, 刘伟堂, 张耀中, 金岩, 冯克, 黄义召, 陈爽, 路兴涛, 高传杰. 安全剂环丙磺酰胺和双苯噁唑酸对玉米苯唑氟草酮药害的解毒作用[J]. 中国农学通报, 2023, 39(23): 88-93.
[7]	肖本泽, 王资霖. 转EPSPS基因抗除草剂水稻恢复系的培育及育种评价[J]. 中国农学通报, 2023, 39(2): 8-15.
[8]	王琳琳, 李雪飞, 李凤, 周方园, 张广志, 张新建. 莠去津降解菌的分离及不同施用方式对土壤修复效果的影响[J]. 中国农学通报, 2023, 39(18): 86-93.
[9]	罗爱国, 冯奕开, 庞琼, 朱敏, 聂萌恩, 杨艳君. 肥料、除草剂和硒配施对谷子品质的影响[J]. 中国农学通报, 2023, 39(18): 11-18.
[10]	蒋春艳, 洪鹏翔, 张双照, 李春维, 周美玲, 李建彬, 江巍. 除草剂对花生生理特性的影响[J]. 中国农学通报, 2023, 39(16): 101-108.
[11]	孙养存, 尹紫良, 葛菁萍. 土壤中重金属污染物的来源及治理方式[J]. 中国农学通报, 2022, 38(6): 75-79.
[12]	曹永清, 刘艳, 张丽慧, 晋婷婷, 任嘉红. 荧光假单胞CLW17菌株对草甘膦的降解及其机制初探[J]. 中国农学通报, 2022, 38(30): 108-117.
[13]	宋佳, 潘妍, 王皙玮, 於丽华. 除草剂阿特拉津在土壤中降解方式的研究现状[J]. 中国农学通报, 2022, 38(25): 90-95.
[14]	吴翠霞, 徐加利, 宋敏, 杨丽娜, 张田田, 马冲. 7种土壤处理除草剂对冬小麦田杂草的防治效果[J]. 中国农学通报, 2022, 38(21): 105-111.
[15]	李秉华, 刘小民, 许贤, 赵铂锤, 李卓霖. 6种除草剂对谷子的安全性和杂草防效[J]. 中国农学通报, 2022, 38(19): 133-138.