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中国农学通报

中国农学通报 ›› 2025, Vol. 41 ›› Issue (2): 91-97.doi: 10.11924/j.issn.1000-6850.casb2024-0401

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

太和香椿根际土壤细菌群落结构多样性分析

赵明侠1,2(), 陈茜1, 刘小丽1, 韦兵1, 周祚琴1, 桑杨利1, 张倩倩1, 屈长青1()   

  1. 1 阜阳师范大学，抗衰老中草药安徽省工程技术研究中心，安徽阜阳 236037
    2 武汉大学药学院，武汉 430071
  • 收稿日期:2024-06-15 修回日期:2024-10-21 出版日期:2025-01-13 发布日期:2025-01-13
  • 通讯作者:
    屈长青，男，1972年出生，陕西西安人，教授，博士，研究方向：皖西北药用植物资源的开发利用、天然活性成份的筛选。通信地址：236037 安徽省阜阳市清河西路100号 生物与食品工程学院，Tel：0558-2595615，E-mail：。
  • 作者简介:

    赵明侠，女，1990年出生，安徽阜阳人，在读博士，研究方向：微生物来源天然产物生物合成和代谢调控。通信地址：430071 湖北省武汉市武昌区东湖路115号 武汉大学药学院，Tel：0558-2591353，E-mail：。

  • 基金资助:
    阜阳师范大学校级自然科学重点项目“香椿根际土壤放线菌多样性及PKS、NRPS基因多样性研究”(2020FSKJ04ZD); 安徽省教育厅自然科学重点项目“生物发酵苹果渣对能繁母猪繁殖性能影响及其机制研究”(2023AH050407); 2020年安徽省科技重大专项项目“太和香椿品种改良及高效栽培关键技术研究与示范”(202003a06020020)

Diversity of Bacterial Community Structure of Toona Sinensis Rhizosphere Soil in Taihe Area

ZHAO Mingxia1,2(), CHEN Qian1, LIU Xiaoli1, WEI Bing1, ZHOU Zuoqin1, SANG Yangli1, ZHANG Qianqian1, QU Changqing1()   

  1. 1 Fuyang Normal University, Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine, Fuyang, Anhui 236037
    2 School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071
  • Received:2024-06-15 Revised:2024-10-21 Published:2025-01-13 Online:2025-01-13

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摘要：

为获得太和香椿种植地土壤细菌群落结构特征，本研究以太和香椿根际土壤为研究材料，通过Illumina HiSeq测序平台对太和香椿根际土壤样品中的细菌16S rDNA V3-V4区序列进行高通量测序，并结合生物信息学分析，揭示了香椿根际土壤细菌群落结构特征。结果表明，共获得1628个操作分类单元(operational taxonomic units, OTUs)，物种注释分别属于26门63纲149目261科512属574种。在门的分类水平上，香椿根际土壤主要优势菌群为变形菌门(Proteobacteria，24.26%)、放线菌门(Actinobacteria，22.97%)和厚壁菌门(Firmicutes，21.50%)。菌群基因功能COG和KEGG注释分析发现，香椿根际土壤菌落能够合成如萜类、II型聚酮类、氨基糖苷类、β-内酰胺类、类固醇类、非核糖体肽等天然产物。太和香椿根际土壤微生物不仅具有多样性，且这些微生物还可以产生结构丰富的活性天然产物，具有发掘新的活性天然化合物的潜力。本研究结果对香椿根际土壤微生物资源的利用、开发具有重要的意义。

关键词: 太和香椿, Illumina HiSeq测序, 根际土壤, 细菌多样性, 天然产物

Abstract:

To investigate the soil bacterial community structure of the Toona sinensis cultivation area in Taihe, soil samples from the rhizosphere of Toona sinensis from Taihe were collected. The 16S rDNA V3-V4 region of soil bacteria was sequenced using high-throughput sequencing technology on the Illumina HiSeq platform, and bioinformatics analysis was performed based on the data. The results showed that a total of 1628 operational taxonomic units (OTUs) were obtained, belonging to 26 phyla, 63 classes, 149 orders, 261 families, 512 genera and 574 species. At the phylum level, the dominant bacteria in the rhizosphere soil of Toona sinensis were Proteobacteria (24.26%), Actinobacteria (22.97%) and Firmicutes (21.50%). COG and KEGG annotation analysis of microbiota gene function showed that rhizosphere soil colonies of Toona sinensis could synthesize natural products, such as terpenoids, type II polyketones, aminoglycosides, β-lactams, steroids, non-ribosomal peptides, etc. These results indicated that the bacteria in the rhizosphere soil of Toona sinensis in Taihe not only had rich diversity but also could produce structure-rich and active natural products. The bacteria in the rhizosphere soil of Toona sinensis had great potential for discovering new active natural compounds. The results of this study are of great significance for the utilization and development of microbial resources in the rhizosphere soil of Toona sinensis.

Key words: Toona sinensis cultivated in Taihe, Illumina HiSeq, rhizosphere soil, bacterial diversity, natural products