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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (19): 137-146.doi: 10.11924/j.issn.1000-6850.casb2026-0016

• 畜牧·动物医学·蚕·蜂 • 上一篇    下一篇

4,8-DHT对杂草的抑制作用和生理生化指标的影响

金真毅1,2(), 任龙2, 杨丽2, 王忠华1, 王强2()   

  1. 1 浙江万里学院生物与环境学院， 浙江宁波 315100
    2 浙大宁波理工学院生物与化学工程学院， 浙江宁波 315100
  • 收稿日期:2026-01-04 修回日期:2026-04-29 出版日期:2026-10-15 发布日期:2026-10-15
  • 通讯作者:
    王强，男，1970年出生，新疆乌鲁木齐人，教授，博士，主要从事植物化学方面的研究。通信地址：315100 浙江省宁波市鄞州区钱湖南路1号 浙大宁波理工学院，E-mail：。
  • 作者简介:

    金真毅，男，2000年出生，硕士研究生，主要从事植物化学方面的研究。通信地址：315100 浙江省宁波市鄞州区钱湖南路1号 浙大宁波理工学院，E-mail：。

  • 基金资助:
    浙江省自然科学基金青年基金项目“4,8-DHT及结构改造物抑制杂草生长的生理生态机制研究”(LQ19C140002); 宁波市科技特派员项目“复方雪参胶囊主要原料质量鉴定与评价体系构建”(2025S214)

Effects of 4,8-DHT on Weeds Inhibition and Physiological-Biochemical Parameters

JIN Zhenyi1,2(), REN Long2, YANG Li2, WANG Zhonghua1, WANG Qiang2()   

  1. 1 College of Biological & Environmental Science, Zhejiang Wanli University, Ningbo, Zhejiang 315100
    2 School of Biological and Chemical Engineering, Ningbo Tech University, Ningbo, Zhejiang 315100
  • Received:2026-01-04 Revised:2026-04-29 Published:2026-10-15 Online:2026-10-15

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摘要： 为探索4,8-二羟基-1-四氢萘酮(4,8-dihydroxy-1-tetrahydronaphthone, 4,8-DHT)的除草作用机制，利用杂草种子萌发实验评估4种浓度的4,8-DHT(0.5、1.0、2.5、5.0 mmol/L)对9种杂草陌上菜(Lindernia procumbens)、牛筋草(Eleusine indica)、鼠尾草(Salvia japonica)、蒲公英(Taraxacum mongolicum)、野燕麦(Avena fatua)、早熟禾(Poa annua)、马唐(Digitaria sanguinalis)、狗牙根(Cynodon dactylon)、稗草(Echinochloa crusgalli)的抑制作用，根据行业标准筛选对照发芽率达标且对4,8-DHT敏感的杂草代表（陌上菜和野燕麦），运用生理生化指标测定和转录组学分析进一步揭示其潜在的作用机理。结果表明，高浓度4,8-DHT(>2.5 mmol/L)对所测试9种杂草种子萌发均具有显著抑制作用，且抑制效应呈浓度依赖性。5.0 mmol/L 4,8-DHT对陌上菜、野燕麦种子萌发抑制率分别达到89.9%、86.3%。生理生化分析表明，随着4,8-DHT浓度升高，陌上菜和野燕麦体内超氧化物酶和过氧化氢酶活性呈现先上升后降低的趋势，而丙二醛和脯氨酸含量持续上升，表明4,8-DHT可能通过干扰抗氧化防御系统和渗透调节物质代谢抑制杂草生理活动。转录组分析进一步发现，4,8-DHT处理陌上菜后产生大量差异表达基因，其中5 mmol/L 处理组（3295个）显著多于0.5 mmol/L处理组（1858个），差异基因主要富集在谷胱甘肽代谢、乙醛酸和二羧酸盐代谢、碳固定途径、植物与病原体互作等通路。研究结果从生理生化及分子水平揭示了4,8-DHT抑制杂草生长的潜在机制，可为其作为植物源新型除草剂的开发与实际应用提供科学指导。

关键词: 4,8-DHT, 种子萌发, 生理生化指标, 转录组分析, 作用机制

Abstract:

To explore the herbicidal mechanism of 4,8-dihydroxy-1-tetrahydronaphthone (4,8-DHT), this study carried out a weed seed germination experiment to assess the inhibitory effects of four concentrations of 4,8-DHT (0.5, 1.0, 2.5, and 5.0 mmol/L) on nine weed species (Lindernia procumbens, Eleusine indica, Salvia japonica, Taraxacum mongolicum, Avena fatua, Poa annua, Digitaria sanguinalis, Cynodon dactylon, Echinochloa crusgalli). In accordance with industry standards, representative weeds (L. procumbens and A. fatua) that meet germination rate criteria and sensitivity to 4,8-DHT were selected. Physiological-biochemical parameters analyses and transcriptomic profiling were employed to further reveal their potential mechanisms of action. The results demonstrated that high concentrations of 4,8-DHT (>2.5 mmol/L) significantly inhibited the seed germination of all nine tested weed species, and the inhibitory effect was concentration-dependent. At 5.0 mmol/L, 4,8-DHT exhibited inhibition rate of 89.9% and 86.3% for L. procumbens and A. fatua, respectively. The physiological-biochemical analyses revealed that as the concentration of 4,8-DHT increased, the activities of peroxidase (POD) and catalase (CAT) in the weeds exhibited a pattern of initially increasing and subsequently decreasing. Meanwhile, the levels of malondialdehyde (MDA) and proline (Pro) continuously increased, indicating that 4,8-DHT might impede the physiological activities of the weeds by interfering with the antioxidant defense system and the metabolism of osmotic regulatory substances. Transcriptomic analysis further disclosed that after treating L. procumbens with 4,8-DHT, a substantial number of differentially expressed genes were generated. Among these, the treatment group at 5 mmol/L (3295 genes) was significantly larger than that at 0.5 mmol/L (1858 genes), and these genes were mainly enriched in pathways including glutathione metabolism, glyoxylate and dicarboxylate metabolism, carbon fixation pathways, and plant-pathogen interactions. The research findings uncovered the potential mechanism by which 4,8-DHT inhibits the growth of weeds at the physiological-biochemical levels, offering scientific guidance for the development and practical application of 4,8-DHT as a novel plant-based herbicide.

Key words: 4,8-dihydroxy-1-tetrahydronaphthone, seed germination, physiological-biochemical parameters, transcriptomic profiling, functional mechanism

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