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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (15): 224-228.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

皇冠草（Echinodorus amazonicus）细胞色素P450相似基因的研究

刘林娟 刘会娟 王晓萍   

  • 收稿日期:2010-11-25 修回日期:2011-03-21 出版日期:2011-06-25 发布日期:2011-06-25
  • 基金资助:

    哈尔滨师范大学骨干教师资助计划

Isolation of Cytochrome P450 Gene-analogues from Echinodorus amazonicus

Xiao-Ping Wang   

  • Received:2010-11-25 Revised:2011-03-21 Online:2011-06-25 Published:2011-06-25

PDF (PC)

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摘要：

为了研究皇冠草基因组内的细胞色素P450基因或与P450基因相似的序列，根据哺乳动物细胞色素P450基因设计8条引物，以皇冠草总DNA为模板，进行PCR扩增。结果表明：共获得了26条扩增片段，片段长度300~1500 bp；将其中三条片段进行测序，BLAST分析表明这些序列与已发表的16种植物的细胞色素P450基因具有一定的相似性。这表明在皇冠草基因组内存在P450基因或者有与P450基因相似的序列。

关键词: 影响因素, 影响因素

Abstract:

In order to study Cytochrome P450 gene of Echinodorus amazonicus, PCR amplification was conducted using 8 primers which were designed according to the gene of mammalian cytochrome P450 genes. 26 amplified fragments were obtained and the length varied from 300 to 1500bp. 3 fragments of them were sequenced. BLAST search analysis revealed that these amplified fragments possessed homologies to other cytochrome P450 genes and proteins in different 16 kinds of plant varieties.