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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (24): 148-153.

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

两种遗传背景下小麦Wx近等基因系面粉及面制品色泽研究

张晓 王灿国 别同德 于春花 赵芸   

  • 收稿日期:2011-04-25 修回日期:2011-06-17 出版日期:2011-09-25 发布日期:2011-09-25
  • 基金资助:

    江苏省自然科学基金;江苏省农业科技自主创新资金项目;江苏省科技支撑计划

Study on Flour and Flour-based Product Colors of Wheat Wx Near-isogenic Lines in Two Different Genetic Backgrounds

  • Received:2011-04-25 Revised:2011-06-17 Online:2011-09-25 Published:2011-09-25

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摘要：

为探索不同Wx基因型对面粉及面制品色泽的影响及色泽改良途径，为糯小麦的育种改良提供理论依据，利用‘扬01-2’和‘扬麦17’2种遗传背景下的8种Wx基因型的近等基因系为材料，对8种基因型面粉、面片色泽及色泽主要影响因素多酚氧化酶(PPO)活性进行研究。结果表明，在面粉色泽上，2个背景下8种基因型间总体上无显著差异，其中以aaBBDD和aabbdd 2种基因型面粉色泽略好；在面片色泽及面条感官评价上，2个遗传背景下均以AAbbdd基因型色泽较好，aabbdd基因型色泽较差。面粉多酚氧化酶活性(PPO)检测结果显示，在2个遗传背景下，全缺失型aabbdd均极端高于其它基因型，其原因需深入研究。糯小麦育种需重视对低PPO活性材料的筛选。

关键词: 施氏假单胞菌, 施氏假单胞菌, Na+/H+逆向转运蛋白, nhaA 基因, 克隆, 表达分析

Abstract:

To explore the influence of different Wx genotypes on flour and flour-made products’ color and improvement measures for darker color. So in this study eight genotypes of Wx near-isogenic lines carrying two different genetic backgrounds (‘Yang01-2’ and ‘Yangmai17’) were used as materials to investigate the color of flour and flour sheet and PPO activity of the eight genotypes. Results showed that flour color and flour sheet color were no significant difference among eight genotypes in both two genetic backgrounds generally. However, aaBBDD and aabbdd genotypes were better than the others in the color of flour. In addition, the AAbbdd genotype was often corresponded to brighter color of sheet and noodle, but aabbdd genotype had the darker color of flour-based products in both genetic backgrounds. The PPO activity of aabbdd genotype in both genetic backgrounds was highest and hence. It was probably the main reason resulting in worse sheet color. The high PPO activity and worse color of flour-based product of aabbdd genotype was also probably caused by Wx protein deletion, which should be further discussed.