欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (2): 53-56.doi: 10.11924/j.issn.1000-6850.2011-2470

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

禽流感病毒H9亚型HN31株NS基因

李新生 黄宗梅 王忠田 张红英 王学兵 焦显芹 崔保安   

  • 收稿日期:2011-08-25 修回日期:2011-10-08 出版日期:2012-01-15 发布日期:2012-01-15
  • 基金资助:

    国家“十一五”科技支撑计划“新城疫等禽用新型疫苗的研制与开发”

Cloning and Sequence Analysis of Avian Influenza Virus H9 Subtype HN31 strain NS Gene

  • Received:2011-08-25 Revised:2011-10-08 Online:2012-01-15 Published:2012-01-15

PDF (PC)

1040

摘要：

为克隆和分析禽流感病毒（H9亚型）HN31株的NS基因的分子特征，通过RT-PCR方法扩增其NS基因，PCR产物与载体pMD19-T Vector连接，转化大肠杆菌DH5α，经蓝白斑筛选，调取阳性菌落，摇菌过夜，提取质粒进行PCR鉴定和酶切鉴定，送阳性重组质粒测序。结果显示RT-PCR产物大小为919 bp，HN31株的NS基因长度为890 bp；其与H6、H9亚型的A型流感病毒的亲缘关系比H5、H7、H3、H1亚型流感病毒更近。

关键词: 农民, 农民, 农村, 信息供给, 调查

Abstract:

In order to study the molecular characteristics of the avian influenza virus (H9 subtypes) HN31 strain’s NS gene, its nucleotides were cloned into pMD19-T Vector by RT-PCR. The ligation product was transformed into the competent E.coli DH5α. The white colony was selected, and identified by PCR and EcoRⅠrestriction enzyme digestion. The positive plasmid was sequenced. The results show that the RT-PCR product is 919bp, HN31 strain’s NS gene length is 890bp. Through the phylogenetic analysis, the kinship with H6, H9 subtype is closer than with H5, H7, H3, H1 subtype of influenza virus.