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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (6): 247-251.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

小分子胶原多肽的抗氧化活性研究

刘高梅   

  • 收稿日期:2011-09-18 修回日期:2011-10-10 出版日期:2012-02-25 发布日期:2012-02-25

Study on Antioxidation Effect of Small Collagen Peptide

  • Received:2011-09-18 Revised:2011-10-10 Online:2012-02-25 Published:2012-02-25

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摘要：

为了研究小分子胶原多肽清除DPPH?自由基、羟自由基(-OH)的能力和抗氧化的能力。采用体外DPPH?自由基和羟自由基(-OH)清除系统测定小分子胶原多肽清除自由基的能力；建立D-半乳糖致衰老的模型小鼠，测定小鼠血液及肝脏组织内的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量。结果表明，1000 kDa以下胶原多肽(CP1)组分在10 mg/mL浓度时，对DPPH?自由基的清除率为80%；40 mg/mL时对-OH的清除率为91.56%。与模型组相比服用CP1的小鼠血清和肝脏丙二醛(MDA)含量显著降低(P＜0.01)；超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力显著升高(P＜0.05)。表明小分子胶原多肽具有显著的抗氧化作用。

关键词: 番茄, 番茄, LeWRKY1, 茉莉酸, 表达特征

Abstract:

To study the scavenging effect of hydroxyl radical and DPPH? and antioxidant capacity of small collagen peptide (CPI). DPPH? and hydroxyl radical scavenging system was adopted to evaluate the scavenging effect; The activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and content of malondialdehyde (MDA) in blood serum and liver tissue in D-galactose treated mice were analyzed. The results showed that the scavenging ability of DPPH? radical was 80% in 10 mg/mL level and the scavenging ability of hydroxyl radical was 91.56% in 40 mg/mL level. Malondi-aldehyde (MDA)content revealed a significant decrease in serum and liver (P＜0.01) and Super Oxidase Dimutase (SOD) and Glutathione Per-oxidase (GSH-Px) activity revealed a significant increase (P＜0.05) than model group. Small collagen peptide (＜1000Da) has obvious antioxidant activity.