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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (24): 306-310.

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

无梗五加果多糖脱蛋白工艺的优化

王晶晶 冯颖 生依灵   

  • 收稿日期:2011-10-12 修回日期:2012-01-19 出版日期:2012-08-25 发布日期:2012-08-25

Optimization of Deproteinization Process of Polysaccharides in Fruits from Acanthopanax Sessiliflorus Seem.

  • Received:2011-10-12 Revised:2012-01-19 Online:2012-08-25 Published:2012-08-25

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摘要：

为获得无梗五加果多糖最佳脱蛋白工艺，采用单因素及正交试验设计，分别对酶法和三氯乙酸(TCA)-Sevag 2种方法进行优化。结果表明：将无梗五加粗多糖液调至pH值6.5，采用质量浓度4%的木瓜蛋白酶40℃下酶解2 h，5000 r/min离心10 min，取上清液加入其等体积TCA-Sevag (1:1，v/v)试剂脱蛋白处理4次，其中Sevag试剂中氯仿:正丁醇=5:1(v/v)，脱蛋白率为95.6%，多糖损失率为21.5%。酶法联合三氯乙酸(TCA)-Sevag脱除蛋白法是一种适合无梗五加果多糖的较高效的除蛋白方法。

关键词: 农药残留, 农药残留

Abstract:

To determine the best process to remove protein from polysaccharides in Fruits of Acanthopanax Sessiliflorus Seem., two methods including enzymolysis and trichloroacetic acid (TCA)-Sevag were optimized by single factor and orthogonal design. The results showed that polysaccharides solution at pH values adjusted to 6.5 was dealt with the mass concentration of 4% papain for 2 h at 40℃, and then the supernatant following by centrifuged for 10 min at 5000 r/min was handed for four times by 1:1 (v/v) TCA-Sevag [chloroform:butanol=5:1(v/v)] reagent for that of equal volume. The deproteinization rate was 95.6% and the loss rate of polysaccharides was 21.5%. The method of enzymolysis with TCA-Sevag had high efficiency in deproteinization.