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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (7): 21-26.

• 林学 园艺 园林 • 上一篇    下一篇

AM真菌对黄檗幼苗的侵染特性比较

范继红   

  • 收稿日期:2011-11-15 修回日期:2012-01-23 出版日期:2012-03-05 发布日期:2012-03-05

Comparison of AM Fungi on the Infection Characteristics to Amur Cork tree Seedling

  • Received:2011-11-15 Revised:2012-01-23 Online:2012-03-05 Published:2012-03-05

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摘要：

为了探索不同基质、不同菌种以及混合菌种接种条件下，AM真菌对黄檗（Phellodendron amurense Rupr.）幼苗的侵染特性，通过盆栽实验，用4种AM真菌接种黄檗一年生实生苗。实验结果表明, 不同基质条件下AM真菌对黄檗的侵染强度差异很大，就本次实验而言，以草炭+蛭石+沙子= 1：0.5：1 比例混合的基质接种效果最好；在此基质上接种的4种AM真菌均能侵染黄檗形成菌根，细胞内均产生了泡囊、丛枝结构，其中以接种G.mosseae和G.diaphanum对黄檗的侵染效果最好，成活率均达到100%，未侵染AM真菌的黄檗幼苗很多不能完成一个生长季就死亡；通过混合菌种接种与单独接种的侵染特性比较，凡是含G.mosseae和G.diaphanum菌种的处理侵染率高，而含有G. versiforme和G. diaphanum的组合侵染率一直比较低，而且降低了G.mosseae和G.diaphanum的侵染效率及成活率。

关键词: 氮肥利用率, 氮肥利用率

Abstract:

In order to explore the differences of VA mycorrhiza fungi on the infection and growth to amur cork tree seedlings which among varied inoculation substrates, strains and mixed strains, the annual seedlings were inoculated with four through potted experiments. The results showed that the seedlings inoculated with arbuscular mycorrhiza in different strains were different obviously to the the growth and development. On the part of this experiment, the result of the combination (Peat: Vermiculite: Sand = 1: 0.5: 1) was better. The mycorrhiza could be formed between amur cork tree and four arbuscular mycorrhiza. It improved the seedlings growth greatly. In this substrate, the mycorrhiza all could be formed between annual seedlings and arbuscular mycorrhiza. Vesicle and arbuscular structures were produced in the cells. Comparing to the other mycorrhiza, inoculation with G. mosseae and G. diaphanum were best. It could promote the height, basal diameters, fresh weights and dry weights of seedlings. Most of the seedlings which were not infected couldn’t finish one growing season. Compared with the infection characteristics between the mixed and single inoculation, G. mosseae and G. diaphanum lead to higher infection rate and it improved seedlings growth. Conversely, the combination with G. versiform and G. diaphanum maintained lower infection rate, and they also reduced the infection efficiency of another.