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中国农学通报 ›› 2019, Vol. 35 ›› Issue (25): 43-53.doi: 10.11924/j.issn.1000-6850.casb18030136

所属专题: 油料作物

• 生物技术科学 • 上一篇    下一篇

基于SSR的四川花生遗传多样性分析

侯 睿, 张小军, 岳福良, 张小红, 李 爽, 刘 行, 张相琼, 李文均   

  1. 四川省农业科学院经济作物育种栽培研究所
  • 收稿日期:2018-03-26 修回日期:2018-06-20 接受日期:2018-06-22 出版日期:2019-09-05 发布日期:2019-09-05
  • 通讯作者: 张相琼
  • 基金资助:
    四川省财政创新提升工程青年基金“花生资源材料特性及遗传多样性研究”(2015QNJJ-004);四川省科技创新苗子工程“基于SSR分子标 记对高油酸花生育种的研究”(2016RZ0021)。

Genetic Diversity Analysis of Peanut in Sichuan Based on SSR Markers

  • Received:2018-03-26 Revised:2018-06-20 Accepted:2018-06-22 Online:2019-09-05 Published:2019-09-05

摘要: [目的]研究旨在了解四川花生资源的遗传多样性及表型性状与分子标记的关联分析,为花生分子育种及资源库构建提供理论依据。[方法]鉴定分析57份不同来源花生资源材料的4个农艺性状及4个品质性状,并利用SSR标记研究57份花生材料的遗传多样性,对SSR标记与表型性状进行聚类及关联分析。[结果]表型性状鉴定结果显示花生资源的8个性状中,6个变异较大、多样性较好。67对SSR引物中,有效引物39对,获得多态性条带53条,平均每个引物扩增1.36条,平均Nei"s 基因多样性0.2288、平均Shannon"s 指数0.3695,最远遗传距离为0.51。SSR分子标记聚类分析将57份资源聚为2大类群,GLM分析发现多个与蛋白质含量、株高、含糖量的关联标记。[结论]研究结果表明,四川不同区域间花生遗传多样性较丰富,品种类型单一,聚类及关联分析结果可为四川花生突破性新品种选育的亲本选择提供提供重要参考。

关键词: 秸秆, 秸秆, 微生物菌剂, 堆肥, 氮变化

Abstract: The aim is to provide a basis for the molecular breeding of peanuts (Arachis hypogaea L.) and the construction of resource library. 57 accessions of peanut from various regions of Sichuan Province were selected as experimental materials, four agronomic traits and four quality traits of the materials were tested and identified, and 67 pairs of SSR markers were used to evaluate the materials, and associated with the phenotypic traits. The results revealed that among the 8 traits of peanut resources, 6 traits had greater variation and better diversity. 39 SSR primers were effective among the 67 SSR primers, and 53 polymorphic bands were amplified with an average of 1.36 loci per primer. The mean of Nei’s gene diversity was 0.2288, and Shannon’s information index was 0.3695. Cluster analysis was conducted with UPGMA. The 57 accessions were divided into two major groups at 0.5200 of genetic similarity coefficient and the maximum genetic distance was 0.51. There were several association markers with protein content, plant height, and sugar content. The research shows that the genetic diversity of peanuts in different regions of Sichuan is relatively abundant. The genetic relationships among resources are not related to their geographical distribution. The characteristic differences between local resources and the main cultivars are obvious, and their genetic relationships are far away.