CRISPR/Cas9系统及其在粮油作物遗传改良中的研究进展

doi:10.11924/j.issn.1000-6850.casb2020-0728

中国农学通报 ›› 2021, Vol. 37 ›› Issue (20): 26-34.doi: 10.11924/j.issn.1000-6850.casb2020-0728

所属专题：生物技术

CRISPR/Cas9系统及其在粮油作物遗传改良中的研究进展

高忠奎¹(), 蒋菁², 韩柱强², 黄志鹏², 熊发前², 唐秀梅², 吴海宁², 钟瑞春², 刘菁², 唐荣华², 贺梁琼²()

¹广西农业科学院科研基地管理处,南宁 530007
²广西农业科学院经济作物研究所,南宁 530007

收稿日期:2020-11-30 修回日期:2021-05-10 出版日期:2021-07-15 发布日期:2021-08-06
通讯作者: 贺梁琼
作者简介:高忠奎,男,1983年出生,吉林东辽人,助理研究员,硕士,研究方向为花生遗传育种。通信地址：530007 南宁市大学东路174号广西农业科学院基地管理处,E-mail：48263823@qq.com。
基金资助:
国家自然科学基金项目“基于CRISPR-Cas9技术的广西地方花生优良品种油酸含量改良研究”(31860386);广西自然科学基金项目“基于CRISPR-Cas9技术的广西地方花生优良品种油酸含量改良研究”(2018GXNSFAA281079);广西农业科学院基本科研业务专项项目“花生优异种质创新与新品种选育”(桂农科2021YT052);广西农业科学院基本科研业务专项项目“基于CRISPR-Cas9技术的广西地方花生优良品种油酸含量改良研究”(31860386)

CRISPR/Cas9 System and Its Research Progress in Grain and Oil Crop Genetic Improvement

Gao Zhongkui¹(), Jiang Jing², Han Zhuqiang², Huang Zhipeng², Xiong Faqian², Tang Xiumei², Wu Haining², Zhong Ruichun², Liu Jing², Tang Ronghua², He Liangqiong²()

¹Scientific Research Base Management Office, Guangxi Academy of Agricultural Sciences, Nanning 530007
²Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007

Received:2020-11-30 Revised:2021-05-10 Online:2021-07-15 Published:2021-08-06
Contact: He Liangqiong

摘要/Abstract

摘要：

CRISPR是细菌或古生菌对入侵到体内的病毒,通过核酸特异性识别、Cas9蛋白酶切割产生的一种天然免疫系统。基于CRISPR/Cas9系统的基因组编辑技术,通过sgRNA介导和Cas9蛋白切割实现对靶基因的定点编辑。因其操作简便、编辑效率高、适用范围广的特点,迅速成为研究植物、动物、微生物基因功能的重要手段。同时,随着大量基因组信息和相应数据库的广泛建立,CRISPR/Cas9技术利用反向遗传学,配合生物信息技术,成为遗传改良、创新特异种质的一种极有效的新手段。本文主要对CRISPR/Cas系统的基本原理、CRISPR/Cas9基因编辑技术的作用机制,以及CRISPR/Cas9技术在粮油作物遗传改良、品种选育研究中取得的进展进行了综述,为遗传育种工作者利用该技术进行遗传改良和品种优化升级育提供有效的参考。

关键词: CRISPR/Cas9, 基因组测序, 基因编辑, 粮油作物, 遗传改良, 研究进展

Abstract:

CRISPR is a kind of natural immune system, which is produced by bacteria or archaea through nucleic acid specific recognition and cas9 protease cleavage, when viruses invade the bacteria or archaea. The genome editing technology based on CRISPR/cas9 system could realize the targeted editing of target genes through sgRNA mediation and cas9 protein cutting. Because of its simple operation, high editing efficiency and wide application range, the technology has rapidly become an important means to study the gene functions of plants, animals and microorganisms. At the same time, with the wide establishment of the large number of genomic information and corresponding databases, CRISPR/Cas9 technology has become a new and very effective way to promote genetic improvement and create special germplasm by using reverse genetics and bio-information technology. In this paper, the basic principle of CRISPR/Cas system, the mechanism of CRISPR/cas9 gene editing technology, the genetic improvement and variety breeding progress of grain and oil crops by using the technology were reviewed. The study could provide effective references for genetic improvement and variety optimization.

Key words: CRISPR/cas9, genomic sequencing, gene editing, grain and oil crop, genetic improvement, research progress

中图分类号:

高忠奎, 蒋菁, 韩柱强, 黄志鹏, 熊发前, 唐秀梅, 吴海宁, 钟瑞春, 刘菁, 唐荣华, 贺梁琼. CRISPR/Cas9系统及其在粮油作物遗传改良中的研究进展[J]. 中国农学通报, 2021, 37(20): 26-34.

Gao Zhongkui, Jiang Jing, Han Zhuqiang, Huang Zhipeng, Xiong Faqian, Tang Xiumei, Wu Haining, Zhong Ruichun, Liu Jing, Tang Ronghua, He Liangqiong. CRISPR/Cas9 System and Its Research Progress in Grain and Oil Crop Genetic Improvement[J]. Chinese Agricultural Science Bulletin, 2021, 37(20): 26-34.

图/表 4

参考文献 56

[1]	Shino Y,Shinagawa H,Markino K,et al.Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product[J].Journal Bacteriology,1987,169(12):5429-5433. doi: 10.1128/jb.169.12.5429-5433.1987 URL
[2]	Barrangou R,Fremaux C,Deveau H,et al.CRISPR provides acquired resistance against viruses in prokaryotes[J].Science,2007,315(5819):1709-1712. pmid: 17379808
[3]	Doudna J A,Charpentier E.The new frontier of genome engineering with CRISPR-Cas9[J].Science,2014,346(6213):1258096. doi: 10.1126/science.1258096 URL
[4]	霍晋彦,李姣,荆雅峰,等.CRISPR/Cas9系统在植物基因功能研究中的应用进展[J].植物生理学报,2019,55(3):241-246.
[5]	Lin Q P,Zong Y,Xue C X,et al.Prime genome editing in rice and wheat[J].Nature Biotechnology,2020,38:582-585. doi: 10.1038/s41587-020-0455-x URL
[6]	欧阳乐军,李莉梅,马铭赛,等.CRISPR/Cas9技术发展及其应用进展[J].西北农林科技大学学报:自然科学版,2019,47(10):1-7.
[7]	沈兰,李健,付亚萍,等.利用CRISPR/Cas9系统定向改良水稻粒长和穗粒数性状[J].中国水稻科学,2017,31(3):223-231.
[8]	邵高能,谢黎虹,焦桂爱,等.利用CRISPR/Cas9技术编辑水稻香味基因Badh2[J].中国水稻科学,2017,31(2):216-222.
[9]	Zhang Y,Bai Y,Wu G H,et al.Simultaneous modification of three homoeologs of Ta EDR1 by genome editing enhances powdery mildew resistance in wheat[J].The plant Journal,2017,91(4):714-724. doi: 10.1111/tpj.2017.91.issue-4 URL
[10]	徐鹏,王宏,涂燃冉,等.利用CRISPR/Cas9系统定向改良水稻稻瘟病抗性[J].中国水稻科学,2019,33(4):313-322.
[11]	柏梦焱,袁珏慧,孙嘉丰,等.基于CRISPR/Cas9基因编辑技术创制大豆gmnark超结瘤突变体[J].大豆科学,2019,38(4):525-532.
[12]	Mojica F J,Diez V C,Soria E,et al.Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria[J].Molecular Microbiology,2000,36(1):244-246. pmid: 10760181
[13]	Lander E S.The heroes of CRISPR[J].Cell,2016,164(1/2):18-28. doi: 10.1016/j.cell.2015.12.041 URL
[14]	Jansen R,Embden J D,Gaastra W,et al.Identification of genes that are associated with DNA repeats in prokaryotes[J].Molecular Microbiology,2002,43(6):1565-1575. pmid: 11952905
[15]	Jinek M,Chylinski K,Fonfara I,et al.A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity[J].Science,2012,337:816-821. doi: 10.1126/science.1225829 URL
[16]	郭晓强.CRISPR-Cas9技术发展史:25年的科学历程[J].自然杂志,2016,38(4):278-286.
[17]	Cong L,Ran F,Cox D,et al.Multiplex genome engineering using CRISPR/Cas systems[J].Science,2013,339(6121):819-823. doi: 10.1126/science.1231143 pmid: 23287718
[18]	MaliI P,Yang L,Eavelt K M,et al.RNA-guided human genome engineering via Cas9[J].Science,2013,339(6121):823-826. doi: 10.1126/science.1232033 URL
[19]	Stemberg S H,Redding S,Jinek M,et al.DNA interrogation by the CRISPR RNA-guided endonuclease Cas9[J].Nature,2014,507(7490):62-67. doi: 10.1038/nature13011 URL
[20]	景润春,卢洪.CRISPR/Cas9基因组定向编辑技术的发展与在作物遗传育种中的应用[J].中国农业科学,2016,49(7):1219-1229.
[21]	Fu Y F,Sander J D,Reyon D,et al.Improving CRISPR-Cas nuclease specificity using truncated guide RNAs[J].Nature Biotechnology,2014,32:279-284. doi: 10.1038/nbt.2808 URL
[22]	Doench J G,Fusi N,Sullender M,et al.Optimized sgRNA design to maximize activity minimize off-target effects of CRISPRCas9[J].Nature Biotechnology,2016,34:184-191. doi: 10.1038/nbt.3437 pmid: 26780180
[23]	Zhang D,Zhang H,Li T,et al.Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases[J].Genome Biology,2017,18(1):191. doi: 10.1186/s13059-017-1325-9 URL
[24]	Lee J K,Jeong E,Lee J,et al.Directed evolution of CRISPR-Cas9 to increase its specificity[J].Nature Communication,2018,9(1):3048. doi: 10.1038/s41467-018-05477-x URL
[25]	Zong Y,Wang Y,Li C,et al.Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion[J].Nature Biotechnology,2017,35(5):438-440. doi: 10.1038/nbt.3811 URL
[26]	王影,李相敢,邱丽娟.CRISPR/Cas9基因组定点编辑中脱靶现象的研究进展[J].植物学报,2018,53(4):528-541.
[27]	Tan Y Y,Athena H Y,Bao S Y,et al.Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity[J].Proceedings of the National Academy of Sciences of the United States of America,2019,42:20969-20976.
[28]	Walton R T,Christie K A,Whittaker M N,et al.Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants[J].Science,2020,368:290-296. doi: 10.1126/science.aba8853 URL
[29]	Feng Z,Zhang B,Ding W,et al.Efficient genome editing in plants using a CRISPR-Cas system[J].Cell Research,2013,23(10):1229-1232. doi: 10.1038/cr.2013.114 URL
[30]	Shan Q W,Wang Y P,Li J,et al.Targeted genome modification of crop plants using a CRISPR-Cas system[J].Nature Biotechnology,2013,31(8):686-688. doi: 10.1038/nbt.2650 URL
[31]	沈兰,华宇峰,付亚萍,等.利用CRISPR/Cas9多基因编辑系统在水稻中快速引入遗传多样性[J].中国科学:生命科学,2017,47(11):1186-1195.
[32]	周文甲.利用CRISPR/Cas9基因编辑技术创造早熟香味水稻[D].北京:中国科学院大学,2017.
[33]	吴明基,林艳,刘华清,等.利用CRISPR/Cas9技术创制水稻温敏核不育系[J].福建农业学报,2018,3(10):1011-1015.
[34]	冯璇,王新,韩悦,等.CRISPR/Cas9介导基因组编辑培育糯稻不育系WX209A[J].基因组学与应用生物学,2018,37(4):1589-1596.
[35]	王子璇,靳亚军,张泗举,等.水稻成花素家族OsDTH11基因的CRISPR/Cas9编辑突变体的创制[J].天津农业科学,2019,25(11):1-6.
[36]	范美英,梅法庭,朱义旺,等.利用CRISPR/Cas9技术创制糯稻新材料[J].福建农业学报,2019,34(5):503-508.
[37]	王延鹏,程曦,高彩霞,等.利用基因组编辑技术创制抗白粉病小麦[J].遗传,2014,36(8):848.
[38]	Zhang Y,Liang Z,Zong Y,et al.Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA[J].Nature Communication,2016,7:12617. doi: 10.1038/ncomms12617 URL
[39]	杜丽君.小麦TaMOCl1基因的启动子分析及CRISPR/Cas9突变体的获得[D].泰安:山东农业大学,2018.
[40]	Liang Z,Chen K L,Li T D,et al.Efficient DNA-freegenome editing of bread wheat using CRISPR-Cas9 ribonucleoprotein complexes[J].Nature Communication,2017,8:14261. doi: 10.1038/ncomms14261 URL
[41]	Liang Z,Chen K L,Zhang Y,et al.Genome editing of bread wheat using biolistic delivery of CRISPR-Cas9 in vitro transcripts or ribonucleoproteins[J].Nature Protocols,2018,13(3):413-430. doi: 10.1038/nprot.2017.145 pmid: 29388938
[42]	Liang Z,Zhang K,Chen K,et al.Targeted mutagenesis in Zea mays using TALENs and the CRISPR/Cas system[J].Journal of Genetics and Genomics,2014,41(2):63-68. doi: 10.1016/j.jgg.2013.12.001 pmid: 24576457
[43]	Svitashev S,Schwartz C,Lenderts B,et al.Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes[J].Nature Communication,2016,7:13274. doi: 10.1038/ncomms13274 URL
[44]	Svitashev S,Young J K,Schwartz C,et al.Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA[J].Plant Physiology,2015,169:931-945. doi: 10.1104/pp.15.00793 pmid: 26269544
[45]	Feng C,Yuan J,Wang R,et al.Efficient targeted genome modification in maize using CRISPR/Cas9 system[J].Journal of Genetics and Genomics,2016,43(1):37-43. doi: 10.1016/j.jgg.2015.10.002 URL
[46]	Char S N,Neelakandan A K,Nahampun H,et al.An Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize[J].Plant Biotechnology J,2017,15(2):257-268. doi: 10.1111/pbi.2017.15.issue-2 URL
[47]	Li C,Liu C,Qi X,et al.RNA-guided Cas9 as an in vivo desired-target matador in maize[J].Plant Biotechnology Journal,2017,15(12):1566-1576. doi: 10.1111/pbi.2017.15.issue-12 URL
[48]	Chen R,Xu Q,Liu Y,et al.Generation of transgene-free maize male sterile lines using the CRISPR/Cas9 System[J].Frontiers in Plant Science,2018,9:1180. doi: 10.3389/fpls.2018.01180 URL
[49]	Yuan M,Zhu J,Gong L M,et al.Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing[J].BMC Biotechnology,2019,24:2-7.
[50]	Li Z,Liu Z B,Xing A Q,et al.Cas9-guide RNA directed genome editing in soybean[J].Plant Physiology,2015,169(2):960-970. doi: 10.1104/pp.15.00783 URL
[51]	Cai Y P,Chen L,Liu X J,et al.CRISPR/Cas9-mediated targeted mutagenesis of GmFTla delays flowering time in soya bean[J].Plant Biotechnology Journal,2018:1-10.
[52]	候智红,吴艳,程群,等.利用CRISPR/Cas9技术创制大豆高油酸突变系[J].作物学报,2019,45(6):839-847.
[53]	吴艳,侯智红,程群,等.大豆GmSPL3基因家族功能初探[J].大豆科学,2019,38(5):694-703.
[54]	Yang Y,Zhu K,Li H L,et al.Precise editing of CLAVATA genes in Brassica napus L. regulates multilocular silique development[J].Plant Biotechnology Journal,2018,16(7):1322-1335. doi: 10.1111/pbi.12872 pmid: 29250878
[55]	Sun Q,Lin L,Liu D,et al.CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L[J].International Joural Molecular Science,2018,19(9):2716.
[56]	Zhai Y,Cai S,Hu L,et al.CRISPR/Cas9-mediated genome editing reveals differences in the contribution of INDEHISCENT homologues to pod shatter resistance in Brassica napus L[J].Theoretical and Applied Genetics,2019,132(7):2111-2123. doi: 10.1007/s00122-019-03341-0 URL

靶基因	启动子	结果		文献及年份
靶基因	启动子	突变率	突变材料类型	文献及年份
ROC5, SPP, YSA	OsU6-2	4.8%~84%	纯合或双等位基因突变植株	Feng et al., 2013
CAO1, LAZY1	OsU3	83.3%~91.6%	纯合突变植株	Miao et al., 2013
OsPDS, OsPMS3, OsEPSPS,OsDERF1, OsMSH1, OsMYB5, OsMYB1, OsROC5, OsSPP, OsYSA	OsU6-2, OsU6	21.1%~66.7%	纯合突变植株	Zhang et al., 2014
DEP1, EP3, Gn1a, GS3,GW2, LPA1, BADH2, Hd1	OsU3	50.0%~100.0%	纯合突变植株	沈兰等,2017b
OsDTH11	OsU3	11.8%	纯合突变植株	王子璇等,2019
Hd2, Hd4, Hd5, Bath2	U3, U6	70%	目标株系	周文甲,2017
Badh2	U6	37.5%	目标株系	邵高能等,2018
GS3, Gn1a	OsU3	68.97%~93.3%	目标株系	沈兰等,2017a
TMS5	--	60%	目标株系	吴明基等,2018
Wx	U6	64.3%	目标株系	冯璇等,2018
Wx	U6	91.1%	目标株系	范美英等,2019
Pita, Pi21, ERF922	U3	75%~85%	目标株系	徐鹏等,2019
OsCDC48-T, OsEPSPS-T, OsLDMAR, OsDEP, OsALS-T	Ubi-1	21.8%	突变体植株	Lin etal, 2020

靶基因	启动子	结果		文献及年份
靶基因	启动子	突变率	突变材料类型	文献及年份
ROC5, SPP, YSA	OsU6-2	4.8%~84%	纯合或双等位基因突变植株	Feng et al., 2013
CAO1, LAZY1	OsU3	83.3%~91.6%	纯合突变植株	Miao et al., 2013
OsPDS, OsPMS3, OsEPSPS,OsDERF1, OsMSH1, OsMYB5, OsMYB1, OsROC5, OsSPP, OsYSA	OsU6-2, OsU6	21.1%~66.7%	纯合突变植株	Zhang et al., 2014
DEP1, EP3, Gn1a, GS3,GW2, LPA1, BADH2, Hd1	OsU3	50.0%~100.0%	纯合突变植株	沈兰等,2017b
OsDTH11	OsU3	11.8%	纯合突变植株	王子璇等,2019
Hd2, Hd4, Hd5, Bath2	U3, U6	70%	目标株系	周文甲,2017
Badh2	U6	37.5%	目标株系	邵高能等,2018
GS3, Gn1a	OsU3	68.97%~93.3%	目标株系	沈兰等,2017a
TMS5	--	60%	目标株系	吴明基等,2018
Wx	U6	64.3%	目标株系	冯璇等,2018
Wx	U6	91.1%	目标株系	范美英等,2019
Pita, Pi21, ERF922	U3	75%~85%	目标株系	徐鹏等,2019
OsCDC48-T, OsEPSPS-T, OsLDMAR, OsDEP, OsALS-T	Ubi-1	21.8%	突变体植株	Lin etal, 2020

物种	靶基因	启动子	结果		文献及年份
物种	靶基因	启动子	突变率	突变材料类型	文献及年份
小麦	TaGASR7、TaDEP1	TaU6	26.0%~26.5%	突变体植株	Shan et al., 2013
	TaMLO	TaU6	--	突变体植株	王延鹏,等,2014
	TaGASR7	TaU6	1.5%~5.0%	突变体植株	Zhang et al., 2016
	EDR1	U3, U6	--	突变体植株	Zhang et al., 2017
	TaMLO	TaU3	1.6%	突变体植株	杜丽君,2018
	TaGW2, TaGASR7	TaU6	21.8%~33.4%	突变体株系	Liang et al., 2017, 2018
	OsCDC48-T, OsEPSPS-T, OsLDMAR, OsDEP, OsALS-T	Ubi-1	2.6%~21.8%	未获得突变体植株	Lin et al, 2020
玉米	ZmIPK	ZmU3	13.1%	未获得突变体植株	Liang et al., 2014
	ALS2	ZmU6	--	突变体植株	Svitashev et al., 2015, 2016
	Zmzb7	ZmU3	19%~31%	突变体植株	Feng et al., 2016
	ZmAgo18a, Zm-Ago18b, Zm-Ago a1, a4	U6	70%~74%	突变体植株	Char et al., 2017
	LG₁	ZmU6	51.5%~91.2%	突变体植株	Li et al., 2017
	MS8	U6	22.78%	突变体植株	Chen et al., 2018

物种	靶基因	启动子	结果		文献及年份
物种	靶基因	启动子	突变率	突变材料类型	文献及年份
小麦	TaGASR7、TaDEP1	TaU6	26.0%~26.5%	突变体植株	Shan et al., 2013
	TaMLO	TaU6	--	突变体植株	王延鹏,等,2014
	TaGASR7	TaU6	1.5%~5.0%	突变体植株	Zhang et al., 2016
	EDR1	U3, U6	--	突变体植株	Zhang et al., 2017
	TaMLO	TaU3	1.6%	突变体植株	杜丽君,2018
	TaGW2, TaGASR7	TaU6	21.8%~33.4%	突变体株系	Liang et al., 2017, 2018
	OsCDC48-T, OsEPSPS-T, OsLDMAR, OsDEP, OsALS-T	Ubi-1	2.6%~21.8%	未获得突变体植株	Lin et al, 2020
玉米	ZmIPK	ZmU3	13.1%	未获得突变体植株	Liang et al., 2014
	ALS2	ZmU6	--	突变体植株	Svitashev et al., 2015, 2016
	Zmzb7	ZmU3	19%~31%	突变体植株	Feng et al., 2016
	ZmAgo18a, Zm-Ago18b, Zm-Ago a1, a4	U6	70%~74%	突变体植株	Char et al., 2017
	LG₁	ZmU6	51.5%~91.2%	突变体植株	Li et al., 2017
	MS8	U6	22.78%	突变体植株	Chen et al., 2018

物种	靶基因	启动子	结果		文献及年份
物种	靶基因	启动子	突变率	突变材料类型	文献及年份
大豆	GmFEI2, GmSHR	AtU6	1.3%~21.0%	未获得突变体植株	Cai et al., 2015
	Gm06g14180,Gm08g02290,Gm12g37050	AtU6, GmU6	3.2%~20.2%	未获得突变体植株	Sun et al., 2015
	DD20, DD43	GmU6	59%~76%	未获得突变体植株	Li et al., 2015
	GmFT2a-SP1 GmFT2a-SP2 GmFT2a-SP2	AtU6	48%,53%,37%	突变体植株	Cai et al, 2018
	FAD2-1A	AtU3,AtU6	--	纯合突变体植株	候智红等,2019
	GmSPL3	AtU3	28.6%	纯合突变体植株	吴艳等,2019
	GmSPL3	GmU6	--	纯合突变体植株	柏梦焱等,2019
油菜	BnCLV	AtU3,AtU6	0%~48.5%	突变体植株	Yang et al., 2017
	BnWRKY11,BnWRKY70	AtU3,AtU6	50%~54.5%	突变体植株	Sun et al., 2018
	BnIND, BnALC	AtU3,AtU6	80.5%~76.6%	纯合突变体植株	Zhai et al., 2019
花生	FAD2	AtU6	21%~44%	未获得突变体植株	Yuan et al., 2019

CRISPR/Cas9系统及其在粮油作物遗传改良中的研究进展

CRISPR/Cas9 System and Its Research Progress in Grain and Oil Crop Genetic Improvement

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 56

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	陈和敏, 肖文芳, 陈和明, 吕复兵, 朱根发, 李宗艳, 李佐. 基于CiteSpace的兰花保鲜研究进展及可视化分析[J]. 中国农学通报, 2023, 39(1): 151-164.
[2]	李星星, 韩芳, 周雪, 苏乐平, 袁宏安. 富硒谷子研究进展[J]. 中国农学通报, 2022, 38(7): 1-6.
[3]	刘鹏, 吴巧花, 舒惠理, 周莉荫, 王小东. 油茶对胁迫因子的响应机制研究进展[J]. 中国农学通报, 2022, 38(7): 24-28.
[4]	颜越, 金荷仙, 王丽娴. 国内外社区花园健康效益研究进展[J]. 中国农学通报, 2022, 38(34): 68-75.
[5]	杨武广, 王君, 温凯, 仇景涛. 中国稻鳖共作技术研究进展与展望[J]. 中国农学通报, 2022, 38(31): 12-16.
[6]	王晴, 方文生, 李园, 王秋霞, 颜冬冬, 曹坳程. 杀线虫剂新品种及作用机制研究进展[J]. 中国农学通报, 2022, 38(30): 100-107.
[7]	孙彬, 王芳, 杨雨春, 王君, 陆志民, 董广志, 史婉玲. 水曲柳研究进展[J]. 中国农学通报, 2022, 38(29): 74-79.
[8]	权莹, 张晓娟, 赵辉, 孙晓敏, 马秀奇. CRISPER/Cas9系统在植物基因组定点修饰及作物遗传育种中的应用研究进展[J]. 中国农学通报, 2022, 38(26): 9-14.
[9]	麻磊, 黄晓君, Ganbat Dashzebegd, Mungunkhuyag Ariunaad, Tsagaantsooj Nanzadd, Altanchimeg Dorjsuren, 包刚, 佟斯琴, 包玉海, Enkhnasan Davaadorj. 不同遥感传感器监测森林虫害研究进展与展望[J]. 中国农学通报, 2022, 38(26): 91-99.
[10]	厉雅华, 张向前, 安祺, 武迪, 刘战勇, 孙峰, 张德健, 高敏, 张国英, 邢俊. 耕地地力评价方法及实践应用研究进展[J]. 中国农学通报, 2022, 38(15): 60-68.
[11]	彭婵, 张新叶, 刘宗坤, 马林江, 陈慧玲. 石斛属植物SSR分子标记的研究进展[J]. 中国农学通报, 2022, 38(13): 36-40.
[12]	吴文彦, 程智超, 李梦莎, 隋心, 曾宪楠. 基于Web of Science的根瘤菌发展研究[J]. 中国农学通报, 2021, 37(9): 109-117.
[13]	崔国梅, 许方方, 李顺峰, 魏书信, 刘丽娜, 王安建. 香菇精深加工及生物活性研究进展[J]. 中国农学通报, 2021, 37(7): 132-137.
[14]	范馨月, 张华, 赵继业, 杜子沫, 丛日晨. 绒毛白蜡耐盐碱响应机制的研究进展[J]. 中国农学通报, 2021, 37(28): 28-34.
[15]	张旭, 胡宝贵. 中国农业节水灌溉技术应用研究进展[J]. 中国农学通报, 2021, 37(26): 153-158.