两种养殖模式下黑鲷肠道及养殖水体菌群结构特征的研究

doi:10.11924/j.issn.1000-6850.casb2020-0324

中国农学通报 ›› 2021, Vol. 37 ›› Issue (27): 155-164.doi: 10.11924/j.issn.1000-6850.casb2020-0324

所属专题：水产渔业

• 水产·渔业 • 上一篇

两种养殖模式下黑鲷肠道及养殖水体菌群结构特征的研究

聂志娟¹(), 邵乃磷¹, 张志伟², 胡佳雯³, 徐跑¹, 徐钢春¹()

¹中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业和种质资源利用重点实验室,江苏无锡 214081
²江苏省海洋水产研究所,江苏省海水鱼类遗传育种重点实验室,江苏南通 226007
³南京农业大学渔业学院,江苏无锡214081

收稿日期:2020-08-02 修回日期:2020-11-05 出版日期:2021-09-25 发布日期:2021-10-28
通讯作者: 徐钢春
作者简介:聂志娟,女,1985年出生,江西新干人,助理研究员,在职博士,主要从事水体微生态与生态养殖研究。通信地址：214081 江苏无锡滨湖区南泉薛家里69号中国水产科学研究院淡水渔业研究中心,Tel：025-85390026,E-mail： niezj@ffrc.cn。
基金资助:
江苏省农业科技自主创新资金项目“海水鲷鱼规模化繁育与池塘工业化养殖关键技术创新”(CX172021)

Intestine of Acanthopagrus schlegelii and Microbial Communities in the Aquatic Water: Study in Two Culture Modes

Nie Zhijuan¹(), Shao Nailin¹, Zhang Zhiwei², Hu Jiawen³, Xu Pao¹, Xu Gangchun¹()

¹Key Laboratory of Freshwater Fisheries and Germplasm Resource Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Wuxi Jiangsu 214081
²Marine Fisheries Research Institute of Jiangsu Province, Jiangsu Key Laboratory for Genetics and Breeding of Marine Fishes, Nantong Jiangsu 226007
³College of Fisheries,Nanjing Agriculture University, Wuxi Jiangsu 214081

Received:2020-08-02 Revised:2020-11-05 Online:2021-09-25 Published:2021-10-28
Contact: Xu Gangchun

摘要/Abstract

摘要：

为了研究池塘流水槽循环水黑鲷养殖模式(IP)和传统池塘黑鲷养殖模式(EP)菌群结构特征,运用高通量测序技术,比较分析2种养殖模式黑鲷肠道和养殖水体中菌群组成结构与功能。结果显示：黑鲷肠道和养殖水体中主要由变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、蓝藻细菌(Cyanobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和绿弯菌门(Chloroflexi)组成,其丰度之和为98.32%~99.22%;在属水平上,菌属差异明显,IP模式下黑鲷肠道显著增加的菌属包括鲁杰氏菌属Ruegeria(14.84%)、聚球藻属Synechococcus(14.24%)、Marivita(3.58%)、norank_o__PeM15(2.97%)、norank_C__KD4-96(2.56%)(P<0.05) (IP>EP);而EP模式下,黑鲷肠道中肠杆菌属Enterobacter(10.31%)、分支杆菌属Mycobacterium(10.30%)、Romboutsia(7.37%)、肠球菌属Enterococcus(6.29%)、乳球菌属Lactococcus(5.38%)显著增加(P<0.05) (IP<EP)。2种模式下黑鲷肠道菌群丰富度和多样性均高于其相应的养殖水体(P<0.05);IP养殖模式水体菌群丰富度和多样性显著高于EP养殖模式(P<0.05),而黑鲷肠道之间无显著性差异(P>0.05)。2种养殖模式条件下菌群结构存在差异,且存在较多丰度差异显著的菌属。IP模式下黑鲷肠道中具有差异显著的优势菌属大多为有益菌,而EP模式黑鲷肠道多为慢性致病菌或条件致病菌属。

关键词: 池塘流水槽循环水养殖, 黑鲷, 菌群结构, 高通量测序, 肠道

Abstract:

To investigate the microbial communities’ structure of water and intestine of black sea bream (Acanthopagrus schlegelii) reared in in-pond raceway culture system (IP) and pond (EP), intestines and culture water were selected as research objects. Using high-throughput sequencing technology, the structure and function of bacterial communities under the two different culture modes were compared and analyzed. The results show that the microbial communities are mainly composed of Proteobacteria, Actinobacteria, Cyanobacteria, Firmicutes, Bacteroidetes and Chloroflexi, and the sum of their abundance is 98.32%-99.22%. At genus level, bacteria are significantly different. Furthermore, the main increased species in IP mode were Ruegeria (14.84%) Synechococcus (14.24%), Marivita (3.58%), norank_o__PeM15 (2.97%) and norank_C__KD4-96 (2.56%)(P<0.05) (IP > EP). However, the main bacteria in EP model are Enterobacter (10.31%), Mycobacterium (10.30%), Romboutsia (7.37%), Enterococcus (6.29%) and Lactococcus (5.38%) (P<0.05) (IP < EP). The bacterial community in intestine has high richness and diversity, IP mode is significantly higher than that in EP mode (P<0.05), but there is no significant difference between fish intestines (P>0.05). There are differences in the structure and function of the flora under the two culture modes, and there are a large proportion of bacterial communities with significant differences in relative abundance. Most of the dominant bacteria with significant differences are beneficial bacteria in the intestine of black sea bream in IP mode, while in the EP mode, the bacteria are mostly chronic or conditional pathogens.

Key words: in-pond raceway culture system, black sea bream, microbial community, high-throughput sequencing, intestine

中图分类号:

S964.9

聂志娟, 邵乃磷, 张志伟, 胡佳雯, 徐跑, 徐钢春. 两种养殖模式下黑鲷肠道及养殖水体菌群结构特征的研究[J]. 中国农学通报, 2021, 37(27): 155-164.

Nie Zhijuan, Shao Nailin, Zhang Zhiwei, Hu Jiawen, Xu Pao, Xu Gangchun. Intestine of Acanthopagrus schlegelii and Microbial Communities in the Aquatic Water: Study in Two Culture Modes[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 155-164.

图/表 4

图1. 两种模式下4组样品中细菌丰富性和多样性 A、B、C、D分别代表基于OTU水平得出的Sobs、Ace, Simpson和Pd指数。BII：IP模式黑鲷肠道组;BEI：EP模式黑鲷肠道组;BEW：EP模式黑鲷养殖水体组;BIW：IP殖模式黑鲷养殖水体组,下同。 *代表0.01 ≤ P ≤0.05,**代表 0.001 ≤P≤ 0.01,***代表P ≤ 0.001

图2. 两种模式下所有样品微生物群落 PCoA排序图(A)和层级聚类分析(B)

图3. 两种模式下4组样品菌群组成（A：门水平,B：属水平）

图4. 两种模式下菌属差异分析肠道和养殖水体(A),肠道样品(B),养殖水体(C)

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两种养殖模式下黑鲷肠道及养殖水体菌群结构特征的研究

Intestine of Acanthopagrus schlegelii and Microbial Communities in the Aquatic Water: Study in Two Culture Modes

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