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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (12): 113-118.doi: 10.11924/j.issn.1000-6850.casb2022-0720

所属专题： 油料作物

• 生物科学 • 上一篇    下一篇

硫素水平对大豆叶片碳氮代谢关键酶活性的影响

王小竟(), 李永平, 宋爽, 周琦, 王鑫, 董守坤()   

  1. 东北农业大学农学院，哈尔滨 150030
  • 收稿日期:2022-08-25 修回日期:2022-10-15 出版日期:2024-04-25 发布日期:2024-04-22
  • 通讯作者:
    董守坤，男，1978年出生，山东泰安人，研究员，博士研究生，研究方向：大豆栽培生理及保护性耕作。通信地址：150030 黑龙江哈尔滨香坊区长江路600号 东北农业大学农学院，Tel：0451-55191277，E-mail：。
  • 作者简介:

    王小竟，女，1999年出生，山东德州人，硕士研究生，研究方向：大豆栽培生理。通信地址：150030 黑龙江哈尔滨香坊区长江路600号 东北农业大学农学院，Tel：0451-55191277，E-mail：。

  • 基金资助:
    国家重点研发计划“逆境条件下大田油料作物全苗壮苗技术研究与示范”(2020YFD1000902)

Effects of Sulfur Level on Key Enzymes Activities in Carbon and Nitrogen Metabolism in Soybean Leaves

WANG Xiaojing(), LI Yongping, SONG Shuang, ZHOU Qi, WANG Xin, DONG Shoukun()   

  1. Agricultural College, Northeast Agricultural University, Harbin 150030
  • Received:2022-08-25 Revised:2022-10-15 Published:2024-04-25 Online:2024-04-22

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摘要：

为了探究不同的硫素水平对大豆苗期和花期叶片碳氮代谢关键酶活性的影响，以大豆品种‘来豆2号’和‘合丰55号’为试材，用浇灌营养液的方法测大豆苗期和花期叶片碳代谢关键酶活性的数据。‘来豆2号’和‘合丰55号’的GOGAT和SS酶活性在160 mg/L处理下达最大值；‘来豆2号’GS酶活性在160 mg/L处理下酶活性峰值最强，‘合丰55号’苗期峰值是260 mg/L处理下达最大值，花期峰值是硫营养最低的20 mg/L处理下；‘来豆2号’SPS酶活性在40 mg/L处理下达最大值，而‘合丰55号’苗期峰值是硫营养较高的80 mg/L处理，花期峰值是硫素水平最高的260 mg/L处理。结果表明，谷氨酸合成酶(GOGAT)、谷氨酰胺合成酶(GS)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)的开花期酶活性显著大于苗期，品种间呈相同规律。

关键词: 硫素水平, 大豆, 碳氮代谢, 酶活性, 苗期, 花期

Abstract:

In order to explore the effects of different sulfur levels on the key enzyme activities in carbon and nitrogen metabolism in soybean leaves at seedling and flowering stages, soybean varieties ‘Laidou 2’ and ‘Hefeng 55’ were used as test materials. The data of key enzyme activities of carbon metabolism in soybean leaves at seedling and flowering stages were measured by watering nutrient solution. The GOGAT and SS enzyme activities of ‘Laidou 2’ and ‘Hefeng 55’ reached the maximum under 160 mg/L treatment. The peak value of GS enzyme activity of ‘Laidou 2’ was the strongest under 160 mg/L treatment, the peak value of ‘Hefeng 55’ in seedling stage was the highest under 260 mg/L treatment, and the peak value in flowering stage was the lowest under 20 mg/L treatment of sulfur nutrition. The SPS enzyme activity of ‘Laidou 2’ reached the maximum value under 40 mg/L treatment, while the peak value of ‘Hefeng 55’ at seedling stage was under 80 mg/L treatment with higher sulfur nutrition, and the peak value at flowering stage was under 260 mg/L treatment with the highest sulfur level. The results showed that the activities of glutamate synthase (GOGAT), glutamine synthetase (GS), sucrose phosphate synthase (SPS) and sucrose synthase (SS) at flowering stage were significantly higher than those at seedling stage, and the same rule was found among varieties.

Key words: sulphur levels, soybean, carbon and nitrogen metabolism, enzyme activity, seedling stage, flowering period