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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (20): 19-28.doi: 10.11924/j.issn.1000-6850.casb2025-0775

• 农学·农业基础科学 • 上一篇    下一篇

不同枸杞品种萌发期和幼苗期耐热指标筛选及耐热性评价

钟楠1(), 牛洁2, 甘晓燕1, 陈虞超1, 田莉1, 石磊1, 秦垦2()   

  1. 1 宁夏农林科学院农业生物技术研究中心， 银川 750021
    2 宁夏农林科学院枸杞科学研究所， 银川 750021
  • 收稿日期:2025-09-21 修回日期:2026-01-11 出版日期:2026-10-25 发布日期:2026-10-26
  • 通讯作者:
    秦垦，男，1971年出生，宁夏人，研究员，主要从事枸杞育种方面的研究。通信地址：750002 宁夏银川市金凤区黄河东路590号，Tel：0951-6886756，E-mail：。
  • 作者简介:

    钟楠，女，1993年出生，副研究员，博士研究生，主要从事植物遗传育种。通信地址：750002 宁夏银川市金凤区黄河东路590号，Tel：0951-6886756，E-mail：。

  • 基金资助:
    宁夏自然科学基金优秀青年项目“基于单细胞测序探索宁夏枸杞热激响应和热激记忆的分子机制”(2026A2566); 中央本部级重大支减项目“药用苦枸杞品种选育研究”(2060302-2203-01); 宁夏回族自治区重点研发项目“枸杞重要品质经济性状功能基因挖掘与解析”(2022BBF01001-02)

Screening of Heat Tolerance Indexes and Evaluation of Heat Tolerance in Different Wolfberry Varieties at Germination and Seedling Growth Stages

ZHONG Nan1(), NIU Jie2, GAN Xiaoyan1, CHEN Yuchao1, TIAN Li1, SHI Lei1, QIN Ken2()   

  1. 1 Ningxia Academy of Agriculture and Forestry, Agricultural Biotechnology Research Center, Yinchuan 750021
    2 Wolfberry Engineering Technology Institute, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750021
  • Received:2025-09-21 Revised:2026-01-11 Published:2026-10-25 Online:2026-10-26

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摘要：

为明确温度对枸杞种子萌发及幼苗生长的影响，构建一套全面、科学的萌发期与幼苗期耐热性综合评价体系，从而为选育耐高温的枸杞品种提供理论依据与方法指导。本研究以14个不同特性的枸杞品种为试验材料，分别设置萌发期(21℃、28℃、35℃、38℃)和幼苗期(25℃、28℃、35℃、38℃)进行了多维度的温度胁迫处理，测定发芽率、发芽势、发芽指数、活力指数、根长、芽长及根冠比等形态指标，计算各指标的耐热系数。采用主成分分析、隶属函数分析和聚类分析等方法，对枸杞品种的耐热性进行综合评价。结果表明：温度低于35℃时，14个品种的种子均能正常萌发；35℃条件下，幼苗根长与芽长生长受到显著抑制；当温度升至38℃时，幼苗基本停止生长，进入热激休克状态。主成分分析将8个单项指标转化为3个相互独立的综合指标，累计贡献率达91.24%，能够全面反映枸杞的耐热性信息。基于耐热性综合评价D值，将14个枸杞品种划分为三大类，其中品种14-02、2-182和7-8表现为耐热类型，而N7、2-184和19009-20为极不耐热类型。本研究建立的综合评价方法操作简便、结果可靠，可快速初步鉴定枸杞品种的耐热性，为耐热枸杞新品种培育提供有效的技术支撑。

关键词: 枸杞, 耐热性, 萌发期, 幼苗期, 相关性分析, 综合评价

Abstract:

To clarify the impact of temperature on seed germination and seedling growth of Lycium barbarum, establish a comprehensive heat tolerance evaluation system for the germination and seedling stages, and provide theoretical and methodological basis for breeding heat-resistant varieties, we selected 14 L. barbarum varieties as experimental materials in this study. We applied four temperature treatments (21℃, 28℃, 35℃, and 38℃) at the germination stage and the seedling stage. Subsequently, we measured morphological indicators, including germination rate, germination potential, germination index, vigor index, root length, shoot length, and root-shoot ratio, and calculated the heat tolerance coefficient for each indicator. Furthermore, we adopted principal component analysis (PCA), membership function analysis, and cluster analysis to comprehensively evaluate the heat tolerance of the L. barbarum varieties. The results showed that all 14 varieties germinated normally at temperatures below 35℃. However, at 35℃, root and shoot elongation was significantly inhibited in seedlings. When the temperature rose to 38℃, seedlings virtually ceased growth and entered a heat-shock state. PCA transformed the eight individual indicators into three mutually independent comprehensive indicators, with a cumulative contribution rate of 91.24%, fully capturing the heat tolerance information of L. barbarum. In conclusion, based on the comprehensive evaluation D value for heat tolerance, we divided the 14 varieties into two major categories: varieties 14-02, 2-182, and 7-8 were classified as heat-tolerant types, whereas varieties N7, 2-184 and 19009-20 were extremely heat-sensitive. The comprehensive evaluation method established in this study is reliable and is simple to operate. It enables rapid preliminary identification of heat tolerance in L. barbarum varieties and provides effective technical support for breeding new heat-resistant L. barbarum cultivars.

Key words: Lycium barbarum, heat tolerance, germination period, seedling stage, correlation analysis, comprehensive evaluation

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