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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (5): 14-14.

所属专题： 生物技术； 小麦

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

黑麦草叶蛋白提取工艺研究

杨超英,刘 艳,薛正莲,杨 浩   

  • 出版日期:2007-05-05 发布日期:2007-05-05

Extraction of Leaf Protein Concentrate(LPC) from Ryegrass and it’s Amino Acid Content

Yang Chaoying, LiuYan, Xue Zhenglian, Yang Hao   

  • Online:2007-05-05 Published:2007-05-05

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摘要： 采用酸化和加热相结合的方法，研究了从黑麦草中提取叶蛋白的最佳工艺条件，并分析了黑麦草叶蛋白中的氨基酸组成。结果表明黑麦草叶蛋白提取的最佳工艺条件为pH4.0，料水比1：2，凝聚温度为70℃，加热时间5min。该条件下提取的叶蛋白含量可高达70.48%。氨基酸分析显示黑麦草叶蛋白中人体必需氨基酸、鲜味氨基酸和药效氨基酸分别占总氨基酸的40.3247%、22.2439%和77.5609%。黑麦草叶蛋白具有很好的营养、保健和药用价值。

关键词: 土壤类型, 土壤类型, 品质性状, 优质强筋小麦, 差异性

Abstract: The optimum conditions of extracting LPC from the fresh leaves of Ryegrass were studied by using acid-heat methods. And the amino acid contents of LPC were determined. The results show that the optimum extraction technique of LPC was as pH 4.0, the ratio 1:2 for fresh leaf to water and 70℃ with 5min. In such condition the protein concentration of LPC can reach 70.48%. The proportion of essential amino acid, sapor amino acid and drug-effective amino acid in total amino acid are 40.3247%, 22.2439% and 77.5609% separately. The LPC from ryegrass have nutritional, healthy and drug-effective value.

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