玉米抗病基因一致性图谱的构建

摘要/Abstract

摘要： 发掘和精细定位玉米抗病基因是构建玉米抗病分子育种技术体系的重要基础。利用生物信息学手段，整理文献和玉米基因组数据库中已有的抗病基因定位的信息，借助高密度玉米分子标记连锁图谱IBM2 2005 neighbors，通过染色体映射的方法，绘制了玉米抗病基因的一致性图谱。结果显示，在试验涉及到的14种主要玉米病害的78个抗病主基因或QTL之中，抗病基因在各条染色体上呈不均匀分布，第3、第6、第10染色体上的主效抗病基因较多，第5和第7染色体上的抗病基因较少，且抗病基因呈簇集分布。研究结果为进一步发掘和鉴定玉米抗病基因和建立玉米抗病分子标记辅助育种技术体系奠定了基础。

关键词: 青玉米, 青玉米, 青莜麦, 产量, 消化能, 冀西北高原, 土壤

Abstract: Identification and fine mapping of resistance genes could provide useful information for setting molecular breeding strategy. In this paper, resistance genes in maize genomic database and references were explored, and the consensus map was constructed with the aid of high-density linkage map IBM 2005 neighbors and the adjacent markers to each major gene or QTL. Consequently, a total of 78 major genes or QTLs involving in resistance to 14 maize diseases were screened out. The results demonstrated that the number of resistance gene distributed on 10 chromosomes asymmetrically, large number of major resistance genes was located on chromosome 3, 6 and 10, and few on chromosome 5 and 7, and resistance gene cluster were found obviously. The results would be valuable for identification of new resistance gene and construction molecular marker-assisted selection strategy for disease resistance in maize.

中图分类号:

S513.034

张书红,张世煌,李新海,席章营. 玉米抗病基因一致性图谱的构建[J]. 中国农学通报, 2007, 23(6): 601-601.

Zhang Shuhong, Zhang Shihuang, Li Xinhai, Xi Zhangying. Construction of Consensus Map of Resistance Gene in Maize[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 601-601.

参考文献

[1] 刘柱,胡新文.分子标记及其在遗传育种中的应用.华南热带农业大学学报,2001,7(1):21-25.
[2] 兰进好,张宝石.玉米分子遗传图谱的SSR和AFLP标记构建.西北农林科技大学学报(自然科学版),2004,32:28-32.
[3] Stich B, Maurer H P, Melchinger A E, et al. Comparison of linkage disequilibrium in elite European maize inbred lines using AFLP and SSR markers. Molecular Breeding, 2006,17: 217-226.
[4] Martin G B, Brommonschenkel S H, Chunwongse J, et al. Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science, 1993, 262:1345-1384.
[5] 王天宇,黎裕.玉米分子图谱的构建和遗传多样性评价.玉米科学,2000,8(2):18-23.
[6] Lawrence C J, Dong Q, Polacco M L, et al. MaizeGDB, the community database for maize genetics and genomics. Nucleic Acids Research, 2004, 32, 393-397.
[7] Hooker A L. A new type of resistance in corn to Helminthosporium turcicum. Plant Dis Rep, 1961,45:780-781.
[8] Hooker A L. A second major gene locus in corn for chlorotic-lesion resistance to Helminthosporium turcicum. Crop Sci, 1977,17:132-135.
[9] Hooker A L. Resistance to Helminthosporium turcicum from Tripsacum floridanum incorporated into corn. MNL, 1981,55(74): 87-88.
[10] Simcox K D, Bennetzen J. Mapping the HtN resistance gene to the long arm of chromosome 8. MNL, 1993,67(187):118-119.
[11] Robbins W A, Warren H L. Inheritance of resistance to Exserohilum turcicum in PI209135, Mayorbela variety of maize. Maydica, 1993,38(3): 209-213.
[12] Bentolina S, Guitton C, Bouvet N. Identification of an RFLP marker tightly linked to the Ht1 gene in maize. Theor Appl genet, 1991,82: 393-398.
[13] Zaitlin D, DeMars S, Gupta M. Linkage of a second gene for NCLB resistance to molecular markers in maize. MNL, 1992,66(116):69-70.
[14] Dingerdissen A L, Geiger H H, Lee M, et al. Interval mapping of quantitative resistance of maize to Setosphaeria turcica, cause of northern leaf blight, in a tropical environment. Mol Breed, 1996,2:143-156.
[15] 尹小燕,王庆华,杨继良,等.玉米大斑病抗性基因Ht2的精细定位.科学通报,2002,47(23):1811-1814.
[16] Van Staden D, Lambert C A, Lehmensiek A, et al. SCAR markers for the Ht1, Ht2, Ht3 and Htn1 resistance genes in maize. Maize Genetics Conference Abstracts, 2001, 43:134.
[17] Chang R Y, Peterson P A. Resistance to Helminthosporium maydis: one gene or two genes? MNL, 1994,68:5-6.
[18] Zaitlin D, DeMars S, Ma Y. Linkage of rhm, a recessive gene for resistance to southern corn leaf blight, to RFLP marker loci in maize (Zea mays) seedlings. Genome, 1993,36:555-564.
[19] Gao Z S, Cai H W, Liang G H. Field assay of seedling and adult-plant resistance to southern leaf blight in maize. Plant Breeding, 2005,124:356-360.
[20] Hagan W L, Hooker A L. Genetics of reaction to Puccinia sorghi in eleven corn inbred lines from Central and South America. Phytopathology, 1965,55:193-197.
[21] Hooker A L. A second major gene locus in corn conditioning resistance to Puccinia sorghi. Phytopathology, 1963, 53:221-223.
[22] Hulbert S, Drake J. Additional loci for rust resistance. MNL, 1995,69(117):98-99.
[23] Delaney D, Webb C A, Hulbert S. A novel rust resistance gene in maize showing overdominance. Mol Plant-Microbe Interact, 1998,11(3): 242-245.
[24] Sudupak M A, Hulbert S. Mechanism of instability of Rp1-J. MNL, 1992,66(119):72.
[25] Wilkinson D R, Hooker A L. Genetics of reaction to Puccinia sorghi in ten corn inbred lines from Africa and Europe. Psychopathology, 1968,58: 605-608.
[26] Sanz-Alferez S, Richter T E, Hulbert S H, et al. The Rp3 disease resistance gene of maize: mapping and characterization of introgressed alleles. Theor Appl Genet, 1995,91:25-32.
[27] Coe E H, Neuffer M G, Hoisington D A. Corn and corn improvement. In：The genetics of corn. Sprangue G F and Dudley J W. American Society of Agronomy: Madison W I, 1988:81-258
[28] Ullstrup A J. Inheritance and linkage of a gene determining resistance in maize to an American race of Puccinia polysora. Phytopathology, 1965,55:425- 428.
[29] Holland J B, Uhr D V, Jeffers D, et al. Inheritance of resistance to southern corn rust in tropical-by-corn belt maize populations. Theor Appl Genet, 1998,72:157-160.
[30] 陈翠霞,杨典洱,王振林,等.齐319携带的南方玉米锈病抗性基因的遗传初析.遗传学报,2002,29(10):903-906.
[31] 刘章雄,刘章雄,王守才,等.玉米P25自交系抗锈病基因的遗传分析及SSR分子标记定位.遗传学报,2003,30(8):706-710.
[32] Cheng Y, Chen J, Chen J P. The complete sequence of a sugarcane mosaic virus isolate causing maize dwarf mosaic virus disease in china. Sciences in china (Series C), 2001,31(6):497-504.
[33] Simcox K D, McMullen M, Louie R. Mapping of multiple disease resistance genes on the short arm of chromosome six. MNL, 1993,67(186):117-118.
[34] Wu J Y, Ding J Q, Du Y X, et al. Identification and molecular tagging of two complementary dominant resistance genes to maize dwarf mosaic virus. Acta Genetica Sinica, 2002,29(12):1095-1099
[35] Wu J Y, Tang J H, Xia Z L, et al. Molecular tagging of a new resistance gene to maize dwarf mosaic virus using microsatellite markers. Acta Botanica Sinica, 2002,44(2):177-180.
[36] Xu M L, Melchinger A E, Xia X C, et al. High-resolution mapping of loci conferring resistance to sugarcane mosaic virus in maize using RFLP, SSR, and AFLP markers. Mol Gen Genet, 1999,261(3):574-581.
[37] Lübberstedt T, Xia X C, Tan G, et al. QTL mapping of resistance to Sporisorium reiliana in maize. Theor Appl Genet, 1999,99:593-598.
[38] 石红良,姜艳喜,王振华,等.玉米抗丝黑穗病QTL分析.作物学报,2005,31:1449-1454.
[39] Lu X W, Brewbaker J L. Molecular mapping of QTLs conferring resistance to Sphacelotheca reiliana (Kühn) Clint. Maize Genetics Cooperation Newsletter, 1999,73:36.
[40] Donahue P J, Stromberg E L, Myers S L. Inheritance of reaction to gray leaf spot in a diallel cross of 14 maize inbreds. Crop Sci, 1991,31:926-931.
[41] Bubeck D M, Goodman M M, Beavis W D, et al. Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci, 1993,33:838-847.
[42] Saghai Maroof M A, Yue Y G, Xiang Z X, et al. Identification of quantitative trait loci controlling resistance to gray leaf spot disease in maize. Theor Appl Genet, 1996,93:539-546.
[43] Lehmensiek A, Esterhuizen A M, van Staden D, et al. Genetic mapping of gray leaf spot (GLS) resistance genes in maize. Theor Appl Genet, 2001,103:797-803.
[44] Pe M E, Gianfranceschi L, Taramino G, et al. Mapping quantitative trait loci (QTLs) for resistance to Gibberella zeae infection in maize. Mol Gen Genet, 1993,241:11-16.
[45] Agrama H A, Moussa M E, Naser M, et al. Mapping of QTL for downy mildew resistance in maize. TheorAppl Genet, 1999,99:519-523.
[46] McMullen M, Simcox K D. Genomic organization of disease and insect resistance genes in maize. Mol Plant-Microbe Interact, 1995,8(66):811-815.
[47] Ming R, Brewbaker J L, Pratt R C, et al. Molecular mapping of a major gene conferring resistance to maize mosaic virus. Theor Appl Genet, 1997,95:271-275.
[48] Kyetere D T, Ming R, McMullen M, et al. Monogenic tolerance to maize streak virus maps to the short arm of chromosome 1. MNL, 1995, 69:136-137.
[49] Zhao H X, Liu X M, Chen M S. H22, a major resistance gene to the Hessian fly (Mayetiola destructor), is mapped to the distal region of wheat chromosome 1DS. Theor Appl Genet, 2006,113:1491-1496
[50] Sarfatti M, Abu-Abied M, Katan J, et al. RFLP mapping of I1, a new locus in tomato conferring resistance against Fusarium oxysporum f. sp. lycopersici race 1. Theor Appl Genet, 1991,82:22-26.
[51] Van der Beek J G, Verkerk R, Zabel P, et al. Mapping strategy for resistance genes in tomato based on RFLPs between cultivars: Cf9 (resistance to Cladosporium fulvum) on chromosome 1. Theor Appl Genet, 1992,84:106-112.
[52] Hmmond-Kosack K E, Jones J D G. Plant disease resistance genes: Unraveling how they work. Canadian Journal of Botany/Revue Canadienne de Botanique, 1996,495-505.
[53] Witsenboer H, Kesseli R V, Fortin M G, et al. Sources and genetic structure of a cluster of genes for resistance to three pathogens in lettuce. Theor Appl Genet, 1995,91:178-188.