桔全爪螨田间种群对双甲脒的敏感性及其两种酶的生化特性

摘要/Abstract

摘要： 分别对采自重庆市北碚、忠县、万州和长寿四个地区的桔全爪螨雌成螨对双甲脒的敏感性进行了生物测定，其LD50分别为0.014、0.031、0.111和0.007 mg/L。同时测定了四个地区桔全爪螨成螨体内羧酸酯酶和乙酰胆碱酯酶的活性，其中羧酸酯酶活性依次为7.78、3.15、6.56和6.00nmol/（min·insect），乙酰胆碱酯酶比活力依次为1.07、0.49、0.50和0.37nmol/（min·mg）。四个地区桔全爪螨体内羧酸酯酶和乙酰胆碱酯酶的动力学常数也存在不同程度的差异。离体抑制作用表明，双甲脒对四个地区桔全爪螨羧酸酯酶和乙酰胆碱酯酶具有明显的抑制作用，当双甲脒浓度达到14.67mmol/L时，抑制作用最强，对北碚、忠县、万州和长寿四个地区桔全爪螨羧酸酯酶的抑制率分别为65.27%、73.84%、81.44%和88.36%，对乙酰胆碱酯酶的抑制率分别为86.84%、84.36%、73.32%和70.85%。

关键词: 芸薹属蔬菜, 芸薹属蔬菜, 镉, 食品安全

Abstract: The bioassay of amitraz on Panonychus citri from Beibei, Zhongxian, Wanzhou, and Changshou was conducted and the LD50 values were 0.014, 0.031, 0.111 and 0.007 mg/L, respectively. Besides, the activities of CarE and AChE from P. citri of different regions were also determined, with corresponding values of 7.78, 3.15, 6.56 and 6.00 nmol/(min·insect) for CarE and 1.07, 0.49, 0.50 and 0.37 nmol/(min·mg) for AChE. Among the four investigated populations, there were significant differences of kinetic parameters for both CarE and AChE. The result of in vitro inhibition study revealed that there were significant inhibitory effects of amitraz on both the two enzymes. The inhibitory effect of amitraz was most obvious at the concentration of 14.67 mmol/L. Under the concentration, the inhibition rates of amitraz on CarE accounted for 65.27%, 73.84%, 81.44% and 88.36%, and those on AChE amounted to 86.84%, 84.36%, 73.32% and 70.85%, respectively.

中图分类号:

S436.661.2＋3

冉春,,陈志永,陈洋,王进军. 桔全爪螨田间种群对双甲脒的敏感性及其两种酶的生化特性[J]. 中国农学通报, 2007, 23(11): 284-284.

Ran Chun, Chen Zhiyong, Chen Yang, Wang Jinjun. Susceptibility to Amitraz and Biochemical Characterizations of Two Enzymes from Panonychus citri Field Populations[J]. Chinese Agricultural Science Bulletin, 2007, 23(11): 284-284.

参考文献

[1] 王代武.柑桔病虫图册（第三版）[M].四川成都:四川科技出版社.1997,76-77.
[2] 黄汉杰.广东省柑桔红蜘蛛的抗药性及防治对策探讨[J].广东省农业科学,1993,(3):37-39.
[3] 方份,卞阿娜,颜亿平.柑桔红蜘蛛对克螨特和氧化乐果的抗性测定[J].福建果树,2004,(3):16.
[4] 宋寿龄,黄光环.桔全爪螨抗药性测定[J].昆虫知识,1989,26(5):293-297.
[5] 黄汉杰,黄媛云,莫禹诗,等.桔全爪螨对常用杀螨剂的抗药性测定[J].广东农业科,1994(3):38-40.
[6] 孟和生,王开运,姜兴印,等.桔全爪螨对常用杀螨剂的抗药性测定[J].农药,2000,39(2):26-28.
[7] 张永毅,邓杏平,魏绍翔.桔全爪螨对水胺硫磷和氧乐果的抗性测定[J].中国柑桔,1993,22(2):37.
[8] 王强,叶兴祥,赵华.桔全爪螨对几种杀螨剂的抗性及药剂混配的增效作用[J].浙江农业科技,1990,1: 15-18.
[9] 陈达荣,钟捷英,陈细生.福州地区桔全爪螨对水胺硫磷抗性测定[J].植物保护,1990,16(3):12-13.
[10] 林荣寿,邓新平,赵志模.四川省柑桔害螨抗药性监测与综合治理[J].西南农业学报,1994,7(3):75-80.
[11] 陈年春,黄华章,彭云良.柑桔全爪螨抗药性监测及主导抗性机制的初步研究[J].农药,1996,35(8)15-18,22.
[12] 陈达荣,钟捷英,陈细生.桔全爪螨体内羧酸酯酶活性与对有机磷抗药性的关系[J].福建农学院学报,1990,19(3):312-315.
[13] 孟和生,王开运,姜兴印,等.桔全爪螨对达螨灵抗性的选育及其生化机理[J].农药学学报,2002,2(3):30-34.
[14] 孟和生,王开运,姜兴印,等.桔全爪螨的抗药性选育及其解毒酶活力变化[J].昆虫学报,2002,45(1):58-62.
[15] 王振荣,李布青.农药商品大全[M].北京:中国商业出版社,1996,260-261.
[16] 陈洋,冉春,熊琳,王进军.应用微量滴度酶标板监测柑桔全爪螨抗药性的研究[J].中国植物保护学会论文集,2005,516-520.
[17] 陈洋,冉春,熊琳,王进军.不同柑桔种质资源对桔全爪螨羧酸酯酶活性及其抗药性的影响[J].西南农业学报,2006,19(3):434-437.