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中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 117-121.

所属专题: 生物技术 食用菌 食用菌

• 食品 营养 检测 安全 • 上一篇    下一篇

藏灵菇源干酪乳杆菌KL1高产胞外多糖发酵条件的优化研究

刘 慧,熊利霞,韩 睿,陈 墨,张红星   

  • 收稿日期:2008-08-27 修回日期:2008-09-10 出版日期:2008-11-05 发布日期:2008-11-05

Studies on the Optimization of the Fermentation Conditions of Exopolysaccharide Produced by Lactobacillus casei KL1 from Tibetan Kefir

Liu Hui, Xiong Lixia, Han Rui, Chen Mo, Zhang HongXing   

  • Received:2008-08-27 Revised:2008-09-10 Online:2008-11-05 Published:2008-11-05

摘要: 本文利用从藏灵菇中筛选的产胞外多糖(EPS)的干酪乳杆菌(Lactobacillus casei)研究提高多糖产量的环境因素。针对主要影响胞外多糖合成的四个因素,采用四因素三水平[L9(34)]正交试验确定了高产胞外多糖优化发酵条件:发酵温度为37℃,发酵时间为12h,培养基起始pH为6.5,接种量为5%。在此优化条件下,胞外多糖的产量是优化前的1.39倍。

关键词: 坡耕地, 坡耕地, 秸秆覆盖, 马铃薯, 培肥效应

Abstract: In this paper, the Lactobacillus casei from Tibetan Kefir was used to screen for improving the environment factors of the yield of exopolysaccharide(EPS). The four factors affecting synthesize of exopolysaccharide of the fermentation conditions were determined by the four factors and three levels orthogonal experiments [L9(3)4]. The result was as follows: fermentation temperature—37℃, fermentation time—12h, origin pH of culture medium—6.5, amount of inoculation—5%. With the optimization conditions,the yield of exopolysaccharide was increased 1.39 times.