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中国农学通报 ›› 2008, Vol. 24 ›› Issue (7): 74-76.

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

海洋红酵母耐盐cDNA文库的构建与序列分析

刘洪博,王宇光,雷禄旺   

  • 收稿日期:2008-03-27 修回日期:2008-04-17 出版日期:2008-07-05 发布日期:2008-07-05

Analysis Sequence and Construct of cDNA Library of Stress Salt from Ocean Rhodotorula

Liu Hongbo, Wang Yuguang, Lei Luwang   

  • Received:2008-03-27 Revised:2008-04-17 Online:2008-07-05 Published:2008-07-05

摘要: 利用消减杂交技术(SSH)构建了海洋红酵母耐盐cDNA文库,一共挑出270个克隆子,选取了25个克隆进行测序,经过Blast分析,初步认为有一个克隆是与耐盐相关的基因,2个属于未知基因,其余为核RNA。

关键词: 调查, 调查, 土地制度, 农村, 中国

Abstract: cDNA library of stress salt from ocean Rhodotorula is constructed with subtraction hybridization, two hundred and seventy clones were picked, and twenty five clones have known sequence, was analysed from Blast, one clone related with tolerance of salt, two clones were unknown gene, others were rRNA

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