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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (10): 15-18.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

低致病性禽流感H9N2病毒感染鸡群引起巴氏杆菌的内源性感染

孙瑞芹,何宏轩,高云英   

  • 收稿日期:2009-02-08 修回日期:2009-03-18 出版日期:2009-05-20 发布日期:2009-05-20

Low pathogenic avian influenza virus (LPAIV) H9N2 inoculation into chickens result in secondary infection of Pasteurella multocida

Sun Ruiqin, He Hongxuan, Gao Yunying   

  • Received:2009-02-08 Revised:2009-03-18 Online:2009-05-20 Published:2009-05-20

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摘要： 摘要：目的 研究低致病性禽流感H9N2病毒感染鸡群的变化。方法 用107.4 EID50/0.1ml H9N2病毒，0.5ml/只，接种1月龄SPF鸡。结果 5日后引起鸡群发病，并引起一些个体发生死亡,剖检发现喉头充血、肺淤血，气囊膜增厚，皮下胶样浸润、出血,腺胃乳头出血，十二指肠出血。肝、脾、肾、肺常见有灰黄色坏死灶。无菌取肺组织做细菌培养时发现在血平板上有白色露珠状菌落，挑取菌落做碱性美蓝染色镜检发现两极浓染的杆状菌；经生化和PCR检验确认该菌属于多杀性巴氏杆菌。结论 低致病性禽流感H9N2病毒感染鸡群可引起巴氏杆菌的内源性感染，从而导致鸡群的高发病率与高死亡率现象。

关键词: 甜菜, 甜菜, 基因工程, 进展

Abstract: Abstracts: Objective To observe the changes of chickens when post-challenge with LPAIV H9N2. Methods One-month-old SPF chickens were inoculated with 107.4EID50/0.1ml H9N2 viruses, 0.5ml/chick. Results Chickens were sick and a few of them were died. At necropsy, the primary gross lesions in all chickens were severe pulmonary consolidation and multifocal hemorrhage in several organs, including subcutaneous, lung, heart, thymus, stomach, intestine, liver, and lymph nodes; Necrotic foci were in liver, spleen, kidney and lung. The bacterial isolated was identified as Pasteurella multocida based on biochemical characteristics and PCR. Conclusion Low pathogenic avian influenza H9N2 virus may cause secondary infection of Pasteurella multocida, which could results of high rate both morbidity and mortality in chickens.