Ca2+参与硝普钠(SNP)对干旱胁迫下小麦幼苗叶片NO水平的调控

摘要/Abstract

摘要： 在15% PEG-6000干旱胁迫下，研究外源一氧化氮(NO)供体硝普钠(SNP)处理对小麦幼苗叶片NO含量、NO合成酶活性的影响及其与Ca2+的关系。干旱胁迫下小麦幼苗叶片NOS活性显著增加，且钙依赖型cNOS快速调控NO产生，但是随着胁迫时间的延长，不依赖钙iNOS活性在NOS活性比例缓慢增加，而NR产生NO的能力只占总NR提取物活性的很小一部分；1mmol/L SNP处理可显著提高干旱胁迫下小麦幼苗叶片NOS和NR活性，诱导NO水平提高，显著缓解膜脂过氧化；用质膜Ca2+通道抑制剂LaCl3与SNP共处理，显著减弱或抵消SNP促进NO合成作用。结果表明，外源NO 显著提高干旱胁迫下小麦幼苗叶片NO合成酶活性和NO含量，有效缓解膜的氧化损伤，而Ca2+参与SNP对干旱胁迫下小麦幼苗叶片NO水平的调控。

关键词: 转化酶, 转化酶, 烟草, 碳代谢

Abstract: Under 15% PEG-6000 Drought stress condition, the effects of sodium nitropprusside (SNP, an exogenous nitric oxide donor) at the concentrations of 1mmol/L SNP on NO content, Nitric Oxide-synthesis enzyme activities and the relation with Ca2+ in wheat seedlings leaves were investigated under drought stress. The result is that 15% PEG could apparently enhance NOS activity in wheat seedling, NO was quickly produced by cNOS, and the proportion of iNOS in NOS was increased slowly after 6h treatments. NO was also induced by NR, which was a little part of total NR activity. The 1mmol/L SNP treatment could apparently alleviate the drought-induced membrane lipid peroxidation, which consistent with the up-regulation of NOS, NR and NO. The effect of SNP was decreased after treatment of blocker of plasma membrane Ca2+ channels LaCl3. The above results indicated that exogenous nitric oxide donor (SNP) could apparently increase Nitric Oxide-synthesis enzyme activities and NO content, and Ca2+ was involved in regulation of NO level in wheat seedling leaves by SNP under drought stress.

李慧,赵文才,赵会杰,韩燕来,谭金芳. Ca2+参与硝普钠(SNP)对干旱胁迫下小麦幼苗叶片NO水平的调控[J]. 中国农学通报, 2009, 25(13): 88-93.

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