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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 242-244.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

一株对美国白蛾高毒力苏云金芽孢杆菌的杀虫基因鉴定

董明1,2,杜立新2,王容燕2,王金耀2,曹伟平2,宋健2,冯书亮2   

  • 收稿日期:2009-11-04 修回日期:2009-11-23 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    国家863计划项目;国家科技支撑项目

Identification of One Bacillus thuringiensis Highly Toxic to Hyphantria cunea

  • Received:2009-11-04 Revised:2009-11-23 Online:2010-02-05 Published:2010-02-05

PDF (PC)

694

摘要：

菌株HYW-8是本实验室从黑龙江伊春市土壤中通过抗生素筛选法分离到的一株形成小菱形晶体的苏云金芽孢杆菌，为了明确其基因型以及杀虫活性，对其进行PCR-RFLP鉴定，并对美国白蛾进行生物活性测定。结果发现菌株HYW-8含有cry1Ab、cry1E基因和一种未知cry2基因。生物测定结果表明，其对美国白蛾具有很高的杀虫毒力，LC50为0.74ug/ml，低于标准菌株HD-1。因此，菌株HYW-8对防治美国白蛾具有较强的应用潜力。

关键词: 高温平菇, 高温平菇, 农艺性状, 评价

Abstract:

Bacillus thuringiensis strain HYW-8 was isolated from the soil in the city of Yichun by antibiotic method.It produced small diamond-shaped crystals. It was identified that contained cry1A,cry1E gene and an unknown cry2 gene by PCR-RFLP.Bioassay showed that the strain HYW-8 had high insecticidal activity to Hyphantria cunea.The LC50 was 0.74ug/ml,which was lower than the standard strain of Bt subsp kurstaki strain HD-1.We suggested that the potential of the strain HYW-8 for used in microbial control of the larvae of Hyphantria cunea.