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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (7): 52-54.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

组培平贝母不同阶段培养体淀粉酶同工酶电泳及其活力的研究

于洋1,朴炫春2,吴松权2,孙丹3,刁艳玲3,廉美兰2   

  • 收稿日期:2009-11-24 修回日期:2009-12-11 出版日期:2010-04-05 发布日期:2010-04-05

Study on the Amylas Isozyme and Activity of in cultures of Fritillaria ussuriensis during different stage of tissue culture

  • Received:2009-11-24 Revised:2009-12-11 Online:2010-04-05 Published:2010-04-05

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摘要：

摘要：以平贝母为供试材料，研究了组培平贝母培养过程中培养体不同发育时期的淀粉酶同工酶及其活力的变化。结果表明：在发育过程中各培养体的淀粉酶同工酶在Rf=0.167处产生了平贝母α -淀粉酶的特征性酶带，在Rf=0.250时，四个时期都有浅蓝色的R-淀粉酶的表达；而β-淀粉酶酶带在鳞茎膨大阶段进行表达，而在其它的三个时期没有该酶的表达。在淀粉酶活力方面，培养体内总淀粉酶活力整体呈下降-上升-下降的趋势，不同发育时期的α -淀粉酶变化趋势与总淀粉酶变化趋势相似，其活力始终高于β-淀粉酶。

关键词: 空间分析, 空间分析, GIS, 地统计学, 植烟土壤, 土壤pH, 土壤中量元素, Kriging

Abstract:

Abstract：The current study taking Fritillaria ussuriensis Maxim. as experiment material, and the research objective were measuring amylas isozyme and activity in cultures of Fritillaria ussuriensis Maxim. during different stage of tissue culture.The results showed that AMY isozyme produced differential enzyme band of Fritillaria ussuriensis α-amylase at Rf=0.167 and light blue R-amylase was expressed during the four phases at Rf=0.250 in cultures during different stage of tissue culture. While enzyme band of β-amylase was only expressed during bulblet expanding. Total amylase activity showed decrease - increase - decrease trend in cultures. The change trend of α-amylase and total amylase was similar, activity of α-amylase was always highter than β-amylase during different stage.