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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (13): 376-379.

所属专题： 生物技术； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

巴西橡胶树茉莉酸诱导胶乳cDNA文库的构建

聂智毅 李先昆 段翠芳 黎瑜   

  • 收稿日期:2010-02-03 修回日期:2010-03-05 出版日期:2010-07-05 发布日期:2010-07-05
  • 基金资助:

    国家自然科学基金;中国热带农业科学院基金项目;中国热带农业科学院橡胶研究所基本科研业务费专项资金

Construction of jasmonic acid -induced expressed cDNA Library of latex in Hevea Brasiliensis

  • Received:2010-02-03 Revised:2010-03-05 Online:2010-07-05 Published:2010-07-05

PDF (PC)

985

摘要：

摘要：以巴西橡胶树热研7-33-97为材料，对橡胶树割线下方施用0.1%茉莉酸，采集胶乳，提取胶乳总RNA并纯化mRNA，构建巴西橡胶树茉莉酸诱导胶乳融合表达文库。检测表明，获得的初级文库滴度为1.9×106 pfu/ml，插入片段在0.5到5kb之间，平均大小为1kb左右；表明该文库质量合格，为今后克隆胶乳中的茉莉酸信号应答基因奠定基础。

关键词: 新农村, 新农村, 庭院绿化, 模式, 效益评价, 人居环境

Abstract:

Abstract：Employing mRNA purified from the total RNA of latex in Clone 7-33-97 Hevea brasiliensis treated by 0.1% jasmonic acid (JA), a JA-induced expressed cDNA library was constructed from latex in Hevea Brasiliensis. The titer of primary library is 1.9×106 pfu/mL. The sizes of inserted fragments in the vector were between 500-5000bp, the average size was about 1 kb. These data indicates that this cDNA Library has a high quality, and therefore provides a useful tool for the cloning of JA responsive genes in latex of Hevea brasiliensis.