关键词: 太行花, 太行花, DNA提取, CTAB法, SDS法, 分子标记

Abstract:

Effect of long term fertilization on the growth of soil microbe in agro-ecosystem was studied, which were provided the theoretic sustainability for the rational fertilizer. The soil samples were collected from the long term in-sit experiment in upland red soil. The content of cultivable bacteria, actinomycetes and fungi in the soil were analyzed. The results showed that the amount of cultivable bacteria, actinomycetes and fungi in soil were increased under long term application of organic manure. Application of phosphorus promoted the growth of cultivable bacteria. Applications of nitrogen fertilizer accelerate the growth of fungi, but restrain the growth of aerobic nitrogen-fixing bacteria. Imbalance fertilization restrained biomass of the soil microbe especially the growth of aerobic cellulose-decomposing bacteria. Application of organic manure was promote to the framework of the soil microbe and was promote to manage the environments of the soil microbe, which improved the production of farmland ecosystem.