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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (22): 50-53.

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

枣树不同品种遗传多样性的RAPD分析

贺润平 渠云芳 雷海英   

  • 收稿日期:2010-04-22 修回日期:2010-07-13 出版日期:2010-11-20 发布日期:2010-11-20
  • 基金资助:

    林业科技支撑计划专题：枣优质高产培育技术研究;枣果实发育及其品质调控研究;特大果型干鲜兼用枣树新品种“金昌一号

RAPD Analysis on Genetic Diversity of Different Zizyphus jujuba Varieties

  • Received:2010-04-22 Revised:2010-07-13 Online:2010-11-20 Published:2010-11-20

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摘要：

为了验证现有枣树材料的亲缘关系，本研究利用RAPD标记对21种枣树品种的遗传多样性进行了分析。结果表明：从36个RAPD引物中，筛选出20个可扩增出清晰且多态性高的条带的引物用于RAPD-PCR扩增，共获得185条DNA片段，其中173条具有多态性，多态率达93.51%，平均每个引物可以扩增出9.25条。利用DPS软件建立了21种枣树品种的聚类分析树状图，聚类分析结果显示：在相似系数82.48处所有枣树品种被聚为一类，在65.98处21种枣树品种被分为两大类群。

关键词: 分子标记, 分子标记, 苹果梨, 分类地位, RAPD

Abstract:

In order to verify the genetic relationship of the Zizyphus jujuba , the genetic diversity of 21 different varieties of Z. jujuba was analyzed by using the random amplified polymorphic DNA(RAPD) in the study. The result showed as follows: 20 effective primers were selected from 36 primers. 185 bands were amplified, among which 173 bands were polymorphic, and percentage of polymorphic bands were 93.51%, an average of approximately 9.25 bands per primer. DPS software was used to construct a cluster analysis dendrogram. Cluster analysis showed that all germplasms used in this study could be clustered together in the threshold of 82.48 distance. 21 different varieties of Z. jujuba were divided into two groups in the threshold of 65.98 distance.