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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (20): 259-262.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

冰糖橙畸形胚状体的生理生化特性研究

蔡小东 张金晶 林丽君   

  • 收稿日期:2010-05-13 修回日期:2010-06-04 出版日期:2010-10-20 发布日期:2010-10-20
  • 基金资助:

    利用转GFP温州蜜柑早期筛选潜在无籽柑橘二倍体胞质杂种

Studies on physiological and biochemical characters of malformed somatic embryoids of Citrus Sinensis L. Osbeck cv. Bingtang

  • Received:2010-05-13 Revised:2010-06-04 Online:2010-10-20 Published:2010-10-20

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摘要：

以冰糖橙胚性愈伤组织为起始材料，进行了胚状体诱导和增殖培养。增殖过程中发现大部分胚状体出现畸形现象，具体表现为莲座型、玻璃化、白化或易愈伤化。为探究这些胚状体发生畸形的原因，本研究比较研究了这四类胚状体部分可溶性物质含量和保护酶活性。结果表明，球形胚状体和易愈伤化胚状体的可溶性糖和蛋白质含量显著高于其它类型胚状体，白化胚状体的两种可溶性物质含量均最低；就SOD活性而言，球形胚状体＞莲座型胚状体＞易愈伤化胚状体＞玻璃化胚状体＞白化胚状体；就POD活性而言，玻璃化胚状体显著高于球形胚状体及其它畸形胚状体。研究结果为了解柑橘畸形胚状体发生的生理生化机制奠定了一定基础。

关键词: 轮作, 轮作, 间作, 连作, 设施栽培, 土传病害, 菌根真菌, 生物多样性

Abstract:

Embryogenic callus of Citrus Sinensis L. Osbeck cv. Bingtang was used as initial explants to explore embryoid induction and multiplication. A majority of embryoids were malformed during the course of the multiplication, such as rosette, vitrification, albino embryoids or embryoids tended to recallus readily. Contents of part of soluble matters and activity of protective enzymes were compared in this study to investigate the factors induced malformed embryoids. The results showed that the soluble sugar and protein in the globular embryoids and embryoids tended to recallus readily were significantly higher than those of others, respectively, and the least contents were in the albino embryoids. For the SOD activity, the globular embryoids＞the rosette embryoids＞embryoids tended to recallus readily＞the vitrified embryoids＞the albino embryoids, whereas The POD activity of the vitrified embryoids was significantly higher than that of the globular embryoids and other types of malformed embryoids.