Abstract: Strawberry mild yellow edge virus (SMYEV) were detected from naturally infected strawberry plants with malformation symptoms from fields based on the study to determine the main pathogen of strawberry viral diseases in Beijing. The expected fragments of about 729 nt include whole CP gene of SMYEV were amplified by RT-PCR and were sequenced by PCR products directly to analyze the molecular variance. Sequence analysis showed 2 different isolates of SMYEV named BJ1 and BJ2 were obtained and the nucleotide identity between them ranged from 82.72% to 82.96%. The sequence of CP gene of different isolates from Beijing, Germany, USA, Czech Republic, Australia, Belgium, Italy, Korea and Shenyang were analyzed. The results showed 33 isolates were divided into 4 groups in which BJ1 and BJ2 belonged to different groups. The identities of nucleotide and deduced amino acid sequences of CP gene between BJ1 and other isolates ranged from 81.10% to 98.35% and 82.17% to 99.17%, and those identities between BJ2 and other isolates ranged from 82.03% to 98.63% and 90.91% to 94.63% respectively.