Analysis of Genetic Relationship of Vitis Germplasm Resources by SSR Markers

doi:10.11924/j.issn.1000-6850.casb15040076

Abstract

Abstract: The aims were to analyze the genetic relationship among 44 Vitis germplasm resources, and provide important basis for cultivar classification, germplasm identification and breeding. SSR markers were employed to clarify the genetic diversity of 44 grape (Vitis L.) genotypes which were suitable for processing of raisins. 8 pairs of polymorphic primers selected from 52 pairs were used for SSR amplification. 190 DNA fragments were generated and all of them were polymorphic bands, and the percentage of polymorphic band was 100%. The ranges of PIC were 0.6868-0.9640, with an average of 0.9084. Jaccard similarity coefficients of the SSR amplification results were analyzed and the genetic similarity coefficient was from 0.63 to 0.92 with the average genetic similarity coefficient of 0.775. The clustering dendrogram constructed by UPGMA method indicated that 44 Vitis materials could be divided into 5 major groups at the similarity coefficient of 0.654. The first group consisted of 22 varieties, including ‘Otilia’ and ‘Heshen Seedless’. The second group consisted of 7 varieties, including ‘Centennial Seedless’ and ‘Queen of Vineyard’. The third group consisted of 5 varieties, including ‘Beauty Seedless’ and ‘Emerala Seedless’. The fourth group consisted of 6 varieties, including ‘Dawn Seedless’ and ‘Blush Seedless’. The fifth group consisted of 4 varieties, including ‘Triumph’ and ‘Perlette’. SSR markers could more accurately detect the genetic background and genetic relationship between the genotypes, of which the materials with distant genetic relationship between major groups or sub-major groups might be used as elite germplasms for further genetic improvement.

CLC Number:

S663.1

Guo Chunmiao,Li Ning,Yang Bo,Zhang Fuchun,Pan Mingqi and Chen Chunsheng. Analysis of Genetic Relationship of Vitis Germplasm Resources by SSR Markers[J]. Chinese Agricultural Science Bulletin, 2015, 31(28): 93-98.

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