Abstract: The aim was to compare the contents of polysaccharides and triterpenes in Ganoderma lucidum from different regions, different growth environments and different growth periods in Hainan. With glucose as the reference substance, anthrone as colour-developing agent, polysaccharides content was measured by 625 nm. With oleanolic acid as the reference substance, vanillin as colour-developing agent, triterpenes content was measured by 550 nm. The results showed that there were some differences of polysaccharides contents and triterpenes contents in Ganoderma lucidum with different sources, artificially cultivated Ganoderma lucidum from Bawangling had the highest polysaccharides content, which was 1.59%, and wild Ganoderma lucidum from Jianfengling had the highest triterpenes content, which was 0.87%. For Ganoderma lucidum of the same origin, under different growth conditions, difference of polysaccharides content was very remarkable (P＜0.01), difference of triterpenes content was remarkable for Jianfengling and Wuzhishan (P＜0.05). For Ganoderma lucidum under the same growth conditions, with different origins, difference of polysaccharides content was very remarkable (P＜0.01), difference of triterpenes content of wild Ganoderma lucidum was remarkable for Jianfengling and Bawangling (P＜0.05). In different growth periods of Ganoderma lucidum, polysaccharides content and triterpenes content were higher in budding period and maturing period, and reduced obviouslly in effete period. Polysaccharides content of artificially cultivated Ganoderma lucidum was more than that of wild Ganoderma lucidum, triterpenes content of most wild Ganoderma lucidum was more than that of artificially cultivated Ganoderma lucidum. Polysaccharides contents and triterpenes contents of Ganoderma lucidum in budding, growing and maturing period were keeping at a higher level, and maturing period was the best harvest period .