Callus Induction of Different Explants of Sinopodophyllum emodi (Wall.) Ying

doi:10.11924/j.issn.1000-6850.casb15100033

Abstract

Abstract: The study aims to establish a technology system for callus induction and culture of Sinopodophyllum emodi (Wall.) Ying, and to select the optimum medium and explants. The authors adopted the orthogonal experimental design to study the effects of MS basic media with 6-BA (6-Benzlaminopurine), 2,4-Dichlorophenoxyacetic acid and TDZ (Thioridazine) and their combinations on induction rate of the explants of leaf and petiole and stem from Sinopodophyllum emodi (Wall.) Ying. The results showed that the callus could be induced from three explants and the leaf was the best explant with a high induction rate of 44.3% and the rate of petiole was 24.7%, the rate of stem was 18.1%. The best suitable medium was MS 6-BA 1.0 mg/L TDZ 0.1 mg/L 2,4-D 1.5mg/L. Combining cytokines of 6-BA and TDZ was beneficial to improve the induction rate and the quality of callus. Compared with the callus of leaf and stem, the loose granular and yellowish green callus from petiole was fit for further inducing differentiation and cell culture. An efficient technology system for callus induction and culture of Sinopodophyllum emodi (Wall.) Ying was established, and it provided the materials for culture of somatic embryogenesis.

CLC Number:

S566.1

Guo Shenghu,Zhu Yongxing and Guan Yajing. Callus Induction of Different Explants of Sinopodophyllum emodi (Wall.) Ying[J]. Chinese Agricultural Science Bulletin, 2015, 31(36): 150-155.

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