Advances in Trehalose Biosynthesis Pathways and Application of Molecular Biology Technique

doi:10.11924/j.issn.1000-6850.casb15120111

Abstract

Abstract: This paper aims to provide better guidance for the construction of trehalose-producing recombinant strains to further improve the yield of trehalose. Research progresses of trehalose biosynthesis pathways and the application of molecular biology technique in trehalose study in recent 30 years, especially the last 10 years, are reviewed. Results show that there are 5 pathways of trehalose synthesis. Although enzymes and genes of trehalose synthesis have been isolated and genetic engineering strains have increased gradually, the improvement of trehalose yield is still inadequate because most recombinant strains are limited to study the physicochemical properties of single enzyme. With the development of modern biological technology, especially the rapid development of DNA recombinant technology, metagenomics and synthetic biology, high expression of heterologous trehalose in recombinant strains will become a hot research topic in the future.

CLC Number:

Q812

Gong Tao,Liu Dehai,Wang Jiwen,Yang Wenling and Xie Fuhong. Advances in Trehalose Biosynthesis Pathways and Application of Molecular Biology Technique[J]. Chinese Agricultural Science Bulletin, 2016, 32(14): 62-67.

References

[1] 杨艳李,李增波.海藻糖TPS/TPP生物合成途径的进化研究[J].科技情报开发与经济,2008,18(16):123-125.
[2] Von Tersch, M.A.B.C. Carlton. Bacteriocin from Bacillus megaterium ATCC 19213: comparative studies with megacin A-216[J].Journal of Bacteriology,1983,155(2):866-871.
[3] 聂凌鸿,宁正祥.海藻糖的生物保护作用[J].生命的化学,2001,21(3):206-209.
[4] 曲茂华,张凤英,何名芳,等.海藻糖生物合成及应用研究进展[J].食品工业科技,2014,35(16):358-362.
[5] Elbein, A.D.Y. PanI, Pastuszak, et al. New insights on trehalose: a multifunctional molecule[J].Glycobiology,2003,13(4): 17R-27R.
[6] Richards, A.S. KrakowkaL, Dexter, et al. Trehalose: a review of properties, history of use and human tolerance, and results of multiple safety studies[J].Food and Chemical Toxicology,2002,40(7):871-898.
[7] 李镭,丁宏标,余晓斌,等.海藻糖酶法合成途径及其酶基因的重组表达研究[J].生物技术通报,2007(3):80-83.
[8] Crowe J. Trehalose. As a “Chemical Chaperone” Fact and Fantasy[A].P. Csermely and L. Vígh. Molecular Aspects of the Stress Response: Chaperones, Membranes and Networks[M].New York, Springer,2007:143-158.
[9] Singer, M.A.S. Lindquist. Multiple effects of trehalose on protein folding in vitro and in vivo[J].Molecular Cell,1998,1(5):639-648.
[10] Kandror, O.A. DeLeonA.L. Goldberg. Trehalose synthesis is induced upon exposure of Escherichia coli to cold and is essential for viability at low temperatures[J].Proceedings of the National Academy of Sciences,2002,99(15):9727-9732.
[11] Tournu, H.A. FioriP. Van Dijck. Relevance of trehalose in pathogenicity: some general rules, yet many exceptions[J].PLoS Pathogens,2013,9(8):e1003447.
[12] Cabib, E.L.F. Leloir. The biosynthesis of trehalose phosphate[J].Journal of Biological Chemistry,1958,231(1):259-275.
[13] De Smet, K.A.A. WestonI.N. Brown, et al. Three pathways for trehalose biosynthesis in mycobacteria[J].Microbiology,2000,146(1):199-208.
[14] Streeter, J.G.A. Bhagwat. Biosynthesis of trehalose from maltooligosaccharides in Rhizobia. Canadian[J]Journal of Microbiology,1999,45(8):716-721.
[15] Higashiyama, T. Novel functions and applications of trehalose[J].Pure and Applied Chemistry,2002,74(7):1263-1269.
[16] Wannet, W.J.H.J.O. den CampH.W. Wisselink, et al. Purification and characterization of trehalose phosphorylase from the commercial mushroom Agaricus bisporus[J].Biochimica et Biophysica Acta (BBA)-General Subjects,1998,1425(1):177-188.
[17] Schiraldi, C.I. Di LerniaM. De Rosa. Trehalose production: exploiting novel approaches[J].Trends in Biotechnology,2002,20(10):420-425.
[18] Qu, Q.S.-J. LeeW. Boos. TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis[J].Journal of Biological Chemistry,2004,279(46):47890-47897.
[19] Ryu, S.-I.C.-S. ParkJ. Cha, et al. A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: molecular cloning and characterization[J].Biochemical and Biophysical Research Communications,2005,329(2):429-436.
[20] Argüelles, J.C. Physiological roles of trehalose in bacteria and yeasts: a comparative analysis[J].Archives of Microbiology,2000,174(4):217-224.
[21] Andersson, U.F. LevanderP. R?dstr?m. Trehalose-6-phosphate phosphorylase is part of a novel metabolic pathway for trehalose utilization in Lactococcus lactis[J].Journal of Biological Chemistry,2001,276(46):42707-42713.
[22] Elbein, A.D.M. Mitchell. Levels of glycogen and trehalose in Mycobacterium smegmatis and the purification and properties of the glycogen synthetase[J].Journal of Bacteriology,1973,113(2):863-873.
[23] Martín, M.C.L.A. DíazM.B. Manzanal et al. Role of trehalose in the spores of Streptomyces[J].FEMS Microbiology Letters,1986,35(1):49-54.
[24] Shimakata, T.Y. Minatogawa. Essential role of trehalose in the synthesis and subsequent metabolism of corynomycolic acid in Corynebacterium matruchotii[J].Archives of Biochemistry and Biophysics,2000,380(2):331-338.
[25] Strom, A.I. Kaasen. Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression[J].Molecular Microbiology,1993,8(2):205-210.
[26] Horlacher, R.W. Boos. Characterization of TreR, the major regulator of the Escherichia coli trehalose system[J].Journal of Biological Chemistry,1997,272(20):13026-13032.
[27] Clegg, J.S.M.F. Filosa. Trehalose in the cellular slime mould Dictyostelium mucoroides[J].Nature,1961,192:1077-1078.
[28] Sussman, A.S.B.T. Lingappa. Role of trehalose in ascospores of Neurospora tetrasperma[J].Science,1959,130(3385):1343-1343.
[29] Goddijn, O.J.K. van Dun. Trehalose metabolism in plants[J].Trends in Plant Science,1999,4(8):315-319.
[30] 陈素丽,彭瑜,周华,等.植物海藻糖代谢及海藻糖-6-磷酸信号研究进展[J].植物生理学报,2014,50(3):233-242.
[31] Lunn, J.E.I. DelorgeC.M. Figueroa et al. Trehalose metabolism in plants[J].The Plant Journal,2014,79(4):544-567.
[32] 于彩虹,卢丹,林荣华,等.海藻糖—昆虫的血糖[J].昆虫知识,2008,45(5):832-837.
[33] Chen, Q.G.G. Haddad. Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals[J].Journal of Experimental Biology,2004,207(18):3125-3129.
[34] Collins, C.H.J.S. Clegg. A small heat-shock protein, p26, from the crustacean Artemia protects mammalian cells (Cos-1) against oxidative damage[J].Cell Biology International,2004,28(6):449-455.
[35] Pellerone, F.S. ArcherC. Behm, et al. Trehalose metabolism genes in Caenorhabditis elegans and filarial nematodes[J].International Journal for Parasitology,2003,33(11):1195-1206.
[36] Xu, J.B. BaoZ.-F. Zhang, et al. Identification of a novel gene encoding the trehalose phosphate synthase in the cotton bollworm, Helicoverpa armigera[J].Glycobiology,2009,19(3):250-257.
[37] Honda, Y.M. TanakaS. Honda. Trehalose extends longevity in the nematode Caenorhabditis elegans[J].Aging Cell,2010,9(4):558-569.
[38] Buts, J.-P.C. StilmantP. Bernasconi et al. Characterization of α, α-trehalase released in the intestinal lumen by the probiotic Saccharomyces boulardii[J].Scandinavian Journal of Gastroenterology,2008,43(12):1489-1496.
[39] ?stergaard, L.N. LarsenH. Leffers, et al. A ribosomal RNA operon and its flanking region from the archaebacterium Methanobacterium thermoautotrophicum, Marburg strain: transcription signals, RNA structure and evolutionary implications[J].Systematic and applied microbiology,1987,9(3):199-209.
[40] Nakada, T.K. MarutaK. Tsusaki, et al. Purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from Arthrobacter sp. Q36[J].Bioscience, biotechnology, and biochemistry,1995,59(12):2210-2214.
[41] Maruta, K.K. HattoriT. Nakada, et al. Cloning and sequencing of trehalose biosynthesis genes from Rhizobium sp. M-ll[J].Bioscience, biotechnology, and biochemistry,1996,60(4):717-720.
[42] Nishimoto, T.M. NakanoT. Nakada, et al. Purification and properties of a novel enzyme, trehalose synthase, from Pimelobacter sp. R48[J].Bioscience, biotechnology, and biochemistry,1996,60(4):640-644.
[43] Tsusaki, K.T. NishimotoT. Nakada, et al. Cloning and sequencing of trehalose synthase gene from Pimelobacter sp. R48[J].Biochimica et Biophysica Acta (BBA)-General Subjects,1996,1290(1):1-3.
[44] Tsusaki, K.T. NishimotoT. Nakada, et al. Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923[J].Biochimica et Biophysica Acta (BBA)-General Subjects,1997,1334(1):28-32.
[45] Lunn J E I, Delorge C M. Figueroa et al. Trehalose metabolism in plants[J].The Plant Journal,2014,79(4):544-567.
[46] 朱玥明,张峻,邢来君.海藻糖合酶的分子生物学研究进展[J].微生物学报,2009(1):6-12.
[47] Wang, J.-H.M.-Y. TsaiG.-C. Lee, et al. Construction of a recombinant thermostable β-amylase-trehalose synthase bifunctional enzyme for facilitating the conversion of starch to trehalose[J].Journal of agricultural and food chemistry,2007,55(4):1256-1263.
[48] Li, Y.X. SunY. Feng, et al. Cloning, expression and activity optimization of trehalose synthase from Thermus thermophilus HB27[J].Chemical Engineering Science,2015(2):323-329.
[49] Siebers, B.B. TjadenK. Michalke, et al. Reconstruction of the central carbohydrate metabolism of Thermoproteus tenax by use of genomic and biochemical data[J].Journal of bacteriology,2004,186(7):2179-2194.
[50] Yan, J.Y. QiaoJ. Hu, et al. Cloning, Expression and Characterization of a Trehalose Synthase Gene From Rhodococcus opacus[J].The protein journal,2013,32(3):223-229.
[51] Jiang, L.M. LinY. Zhang, et al. Identification and characterization of a novel trehalose synthase gene derived from saline-alkali soil metagenomes[J].PloS one,2013,8(10):e77437.