Silkworm Chemosensory Protein CSP9: Antibody Preparation and Expression Analysis

doi:10.11924/j.issn.1000-6850.casb20190400023

Abstract

Abstract: In order to prepare the polyclonal antibody against CSP9 of silkworm, and study the expression characteristics and functions of CSP9 in silkworm. The csp9 gene of silkworm was amplified by PCR, and its prokaryotic expression vector was constructed. The expression of CSP9 protein was induced and purified. The purified CSP9 protein was immunized to New Zealand purebred rabbit to prepare polyclonal antibody. The expression of CSP9 in silkworm was detected by Western blot. Finally, the prokaryotic expression vector of csp9/pET-28a was constructed successfully, and the CSP9 protein with predicted molecular weight was purified. The polyclonal antibody against CSP9 was successfully prepared. The result of Western blot showed that CSP9 was specifically expressed in different stages and tissues of silkworm. In this study, CSP9 protein was successfully expressed and purified, its polyclonal antibody was successfully prepared. The expression characteristics of CSP9 in silkworm were analyzed, which laid a foundation for further study on the function and regulation of CSP9 in silkworm.

CLC Number:

Q812

王景娅,孙妍妍,,, and . Silkworm Chemosensory Protein CSP9: Antibody Preparation and Expression Analysis[J]. Chinese Agricultural Science Bulletin, 2019, 35(34): 44-49.

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