Induced Expression and Identification of Recombinant Protein of Bovine Inhibin pET32a-matpeptide

doi:10.11924/j.issn.1000-6850.casb16060051

Abstract

Abstract: To expressed and purified good immune original of Bovine Recombinant Inhibin pET32a-matpeptide protein, the research induced and expressed Inhibin recombinant protein by determining the induction time, temperature and IPTG induced concentration, through the restructuring of the small protein expression to determine the target protein to soluble and inclusion body protein exists, by the recombinant protein expression to purify the target protein and by Western blot to identify the protein"s immunogenicity. The results showed that the use of 1MIPTG, 18℃ to induce the expression of 18h was induced to express the recombinant inhibin protein, the recombinant protein epsilon expression for determining protein exists in a soluble form and by Western blot to identify the protein has good immunogenicity. The Inhibin pET32a-matpeptide recombinant protein was induced to express soluble high immunogenicity, which laid the foundation for the preparation of anti-Inhibin α subunit antibody.

CLC Number:

Q812

References

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