Establishment and Optimization of Callus Induction System from the Epicotyls Callus of Peanut

doi:10.11924/j.issn.1000-6850.casb16010064

Abstract

Abstract: The objectives were to screen the optimal medium for inducing the epicotyls callus of peanut, establish and optimize the callus-induction system of peanut epicotyls. Different types of peanut varieties were used in this experiment. The epicotyls were used as explants. The epicotyls were cultured on MS-B5 medium supplemented with 3-6 mg/L Pic and 5-10 mg/L 2, 4-D to induce callus. The induction differences of callus were compared with different preculture time, different genotypes, and different plant hormones concentrations, respectively. The results showed that Spanish type‘Baisha 1016’with preculture for 0 day had the biggest inductivity, the optimal medium was MS B5 Pic 3 mg/L, the subculture medium was MS Pic 3 mg/L Gln 1 g/L. This system is suitable for the induction of most peanut varieties and repetitive somatic embryogenesis can be obtained by repeated subculture.

CLC Number:

S565.2

臧新 and . Establishment and Optimization of Callus Induction System from the Epicotyls Callus of Peanut[J]. Chinese Agricultural Science Bulletin, 2016, 32(21): 75-78.

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