Abstract: A field experiment was conducted to investigate the effect of biochar and organic fertilizer application on Artemisia selengensis yield and soil properties in Baguazhou street, Nanjing. Five treatments were set in this experiment: (1) control (CK); (2) 22.5 t/hm2 biochar (CB); (3) 22.5 t/hm2 organic fertilizer (CM); (4) 22.5 t/hm2 biochar + 22.5 t/hm2 organic fertilizer (CBM); (5) 11.25 t/hm2 biochar + 11.25 t/hm2 organic fertilizer (CBM1/2). Results showed that biochar or organic fertilizer application alone had no obvious effect on the biomass, yield and root biomass of Artemisia selengensis. Biochar application alone (CB) increased the concentration of soil organic carbon, total nitrogen, available phosphorus and available potassium by 32%, 7% 16% and 80% compared with that of CK, respectively; while it had no effect on soil pH value and electrical conductivity. Compared with CK, the application of organic fertilizer increased soil total nitrogen and available phosphorus by 12% and 16%; while it had no effect on soil pH value, soil organic carbon, available potassium and CEC. The combined application of biochar and organic fertilizer(CBM) increased soil organic carbon, total nitrogen and available potassium. When the application amount of biochar and organic fertilizer reduced to a half (CBM1/2), soil organic carbon, total nitrogen, available potassium and available potassium also increased significantly. Despite soil fertility increased greatly, the application of biochar and organic fertilizer had no effect on the, biomass, yield and root biomass of Artemisia selengensis. Our results suggested that soil fertility was not the main factor that regulating Artemisia selengensis production in the study area.