Abstract: The study aims to understand the variation characteristics of agricultural climate resources in the river source region of eastern Tibet, and to provide references for government departments to rationally utilize climate resources. Using the daily temperature, sunshine hours and precipitation data in the river source region of eastern Tibet from 1980 to 2015, we analyzed the temporal and spatial distribution of temperature, sunshine hours and precipitation and the impacts of climate change on agriculture in recent 36 years by the application of linear-trend estimate, Mann-Kendall method and so on. The results showed that: the variation trend of annual mean temperature, sunshine hours and precipitation increased at a rate of about 0.38℃/10 a, 22.2 h/10 a, 9.4 mm/10 a, respectively, in the river source region of eastern Tibet; the annual and seasonal mean temperature had the same variation trend, sunshine hours and precipitation presented annual and seasonal differences in different weather stations; the change rate of precipitation in spring was the largest, and that in summer was the least; for temperature≥0℃, the beginning date was advanced, the ending date was postponed, and the lasting days and the accumulated temperature steadily increased. In conclusion, the change of agricultural climate resources will have a huge impact on agricultural development in the river source region of eastern Tibet.