The growth and photosynthetic physiological responses of Phellodendron amurense seedlings under supplementary UV-B radiation

doi:10.11924/j.issn.1000-6850.casb17010077

Abstract

Abstract: To study the ultraviolet radiation effect on growth and photosynthetic physiological mechanism of medicinal plants, treatments were set to supplement UV-B radiation for 40 days onPhellodendron amurense seedlings, the control treatment (CK) was natural sun light, T1 treatment supplemented UV- B radiation intensity of 3.26 μW/cm2, T2 treatment supplemented UV-B radiation intensity of 9.78 μW/cm2. The results showed that: compared with the control treatment, plant biom ss growth and root shoot ratio were significantly lower afterP. amurense seedlings were exposed under different UVB radiation intensities (P <0.05), and both were reduced with the increase of radiation intensity. Photosynthetic rate, transpiration rate and stomatal conductance were significantly lower than those of the control (P <0.05), and decreased with the increase of radiation intensity; Initial fluorescence (F 0), maximum fluorescence (Fm ), PSⅡ original light energy conversion efficiency (Fv /Fm ), PSⅡ potential photochemical activity coefficient(Fv /F 0) and photochemical quenching (qP ) and electron transport rate (ETR ), all decreased (P <0.05) with the increase of UV-B radiation intensity, onphotochemical quenching coefficient (qN ) raised at low radiation intensity, but decreased with the radiation intensity increase, and still higher than the control (P <0.05). Photosynthetic pigment content, including chlorophyll a, chlorophyll b, total chlorophyll, carotenoid and chlorophyll a/b, decreased with the increment of supplementary UV- B radiation intensity. UV- B radiation treatment inhibited the seedlings’growth of P. amurense , reduced the content of photosynthetic pigment and transmission electron ability of PSⅡ reaction center, which led to the decrease of the photosynthesis intensity and had dose effect.

CLC Number:

S567.1

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