Dwarfing Mechanism of Apple Dwarf Rootstock: Research Progress

doi:10.11924/j.issn.1000-6850.casb17070021

Abstract

Abstract: For apple, cultivation under dwarf and closing planting is the development trend of modern production, but the dwarfing mechanism of rootstock which is the main way to realize it has not been revealed, revealing the mechanism is very significant for improving dwarf breeding and cultivation techniques. Dwarfing mechanism of apple rootstock were summarized in dwarf gene, anatomy structure, moisture content, substances transportation, photosynthetic characteristics, enzymes and phenolic, hormones, the dwarfing place and mechanism were discussed. It was summarized and pointed out that apple rootstock dwarfing was the comprehensive effect of graft cut, rootstock xylem and phloem, that grafted shoot as dwarf rootstock was the main dwarfing place, that apple dwarf rootstock might be play the role by affecting root growth. In the future, we should more precisely and more microscopically compare the difference between shoot anatomy of high rootstock and dwarf rootstock by advanced micro-instruments and measuring software, analyze the physiological and biochemical changes of different dwarf rootstock during metabolism of water, mineral nutrition, assimilate in condition of as much consistent environment and material as possible, study further systematically the role played by material synthesis, signal transduction, degradation and interaction of plant hormones in dwarfing, then study and verify the raised problem by research from many aspects, for explaining the dwarfing mechanism of rootstock more clearly. Key words: apple; dwarf stock; dwarfing mechanism

CLC Number:

S661.1

References

[1] 韩振海. 苹果矮化密植栽培—理论与实践[M]. 北京：科学出版社,2011:6,8,15,17.
[2] 张鹤. 苹果砧木M9作自根砧或中间砧的致矮机理研究[D]. 北京: 中国农业大学, 2013: 1,6,36.
[3] 毕晓颖,吴禄平,安利佳. 一个与苹果属显性矮生主基因Dw连锁的RAPD标记[J].园艺学报,2002,29(1):1-4.
[4] 王彩虹, 王倩, 戴洪义,等. 苹果柱型基因Co的一个AFLP标记的SCAR转换[J]. 园艺学报,2002, 29(2)：100-104.
[5] 石丽雪. 苹果矮化基因Md连锁标记的筛选及染色体定位[D]. 泰安: 山东农业大学,2006.
[6] 廖娇, 黄春辉, 辜青青,等. 植物矮化相关基因研究进展[J]. 生物技术通讯, 2011,22(4):593-597
[7] 马丽, 娄喜艳．不同类型砧木对“ 红富士” 苹果地下及地上部分生长的影响[J]．北方园艺, 2013(16):13-17.
[8] 侯玉珏．矮化‘ 富士’ 苹果叶片形态特征与部分生理指标研究[D]. 陕西杨凌: 西北农林科技大学,2012．
[9] 隗晓雯．苹果砧木矮化性评价指标的研究及应用[D]. 河北保定: 河北农业大学,2014．
[10] 杨廷桢,高敬东,王骞,等. 不同中间砧嫁接苹果品种叶片和枝条解剖结构与矮化性关系的研究[J]. 中国农学通报, 2015,31(13):95-99.
[11] 王中英, 解思敏, 杨佩芳.苹果砧木矮化性状的综合解剖学结构[J]. 园艺学报, 1983(2)：20-24.
[12] 王中英, 解思敏,杨佩芳, 等. 苹果矮砧解剖构造研究[J]. 果树学报, 1988,5(1)：6-10.
[13] 赵同生, 陈东玫, 赵永波, 等. 苹果矮化砧木叶片解剖结构研究河北农业科学, 2010, 14(10): 22-23.
[14] Chen B Y, Wang C H, Tian Y K, etal. Anatomical characteristics of young stem and mature leaves of dwarf pear[J]. Scientia Horticulturae, 2015,186: 172-179.
[15] Michael P P, Bruce A O, Derek T M. Rapid predictions of cold tolerance in Douglas-fir seedings using chlorophyll fluorescence after freezing[J]. New Forests,2004,28(1):49-62.
[16] 杨传友, 史金玉, 杜欣阁,等. 苹果叶片气孔的研究[J]. 山东农业大学学报(自然科学版), 1998(1)：8-14.
[17] 罗静. 苹果矮化中间砧矮化效应的研究[D]. 陕西杨凌: 西北农林科技大学, 2013.
[18] 罗静, 王飞, 韩明玉, 等. 2种苹果中间砧致矮的解剖结构机理研究[J]. 西北农林科技大学学报(自然科学版), 2013, 41(6): 124-132.
[19] 王中英, 白瑞琴, 吕晓燕. 矮化中间砧和乔砧苹果树水势变化研究[J]. 园艺学报, 1997,24(2):191.
[20] 韩晓毓, 张林森, 王俊峰, 等. 不同苹果矮化砧木导水特性与水通道蛋白基因表达对干旱胁迫的响应西北农业学报, 2015, 24(10): 109-117.
[21] 张林森, 胥生荣, 张永旺, 等. 干旱胁迫下不同中间砧嫁接苹果苗的导水特性[J]. 园艺学报, 2013,40(11):2137-2143.
[22] 刘魁英, 赵宗芸, 王海兴. ABA,GA3渗透势和压力势对苹果砧木叶片中IAA氧化酶活性的影响[J]. 沈阳农业大学学报, 1993,24(1):50-53.
[23] 史宝胜, 徐继忠, 陈海江, 等. SH系砧木及对应中间砧红富士苹果枝条水分输导阻力研究[J]. 河北林果研究, 2001,16(2):136-139.
[24] 古润泽. 不同砧木对红星苹果叶内矿质元素含量影响的研究[J]. 落叶果树, 1987(2)：11.
[25] 宋晓敏. 苹果苗木质量评价与砧穗组合对幼树生长的影响[D].陕西杨凌: 西北农林科技大学, 2014.#$NL
[26] 李海燕. 不同矮化中间砧华红苹果幼树生理特性的研究[D].北京: 中国农业科学院, 2013. #$NL
[27] 李海燕, 赵德英, 袁继存, 等. 矮化中间砧对华红苹果致矮机理初探[J].中国果树, 2013(3):18-20.
[28] 顾曼如, 张若杼, 黎文文, 等. 苹果植株中的无机氮与游离氨基酸[J].落叶果树, 1979(3): 8-14.
[29] 王中英, 赵玉军, 童德中. 矮化中间砧苹果树14C同化物质分配和运转的研究[J]. 山西农业科学, 1998,26(3):10-14.
[30] Ferree M, Barden J A. The influence of strains and rootstock on photosynthesis, respiration and morphology of‘delicious’apple tree[J]. Soc Hort Sci, 1971, 96(4):453-457.
[31] 王中英, 解思敏, 杨佩芳, 等. 矮砧苹果树光合速率变化研究[J]. 华北农学报, 1990, 5(3): 89-93.
[32] 张建光, 刘玉芳, 施瑞德. 不同砧木上苹果品种光合特性比较研究[J]. 河北农业大学学报, 2004, 27(5):31-33,40.
[33] Zhou Y Q, Qin S J, Ma X X, et al. Effect of interstocks on the photosynthetic characteristics and carbon distribution of young apple tees during the vigorous growth period of shoots[J]. European Journal of Horticultural Science, 2015,80(6):296-305.
[34] 徐继忠, 史宝胜, 马宝焜,等. 苹果不同矮砧与其对应中间砧植株POD、IOD酶活性的研究[J]. 中国农业科学, 2002,35(4):415-420.
[35] 赵家(马攵一水), 王志常, 王西玲, 等. 过氧化物酶同工酶鉴定苹果砧木矮化程度研究[J]. 陕西农业科学, 1986(6):12-15.
[36] 张志华, 刘新彩, 王红霞, 等. 核桃IOD和POD酶活性与生长势的关系[J]. 园艺学报, 2006,33(2): 229-232.
[37] 王宏伟. 甜樱桃矮化砧木矮化机理研究[D]. 泰安：山东农业大学, 2004.
[38] 李文斌, 张映南, 刘庚峰,等. 柑桔矮化砧及半矮化砧过氧化物酶同工酶及活性的比较[J]. 园艺学报, 1989,16(4):261-266.
[39] 胡国谦, 张谷雄, 周中建, 等. 柑桔砧木矮化性与叶片过氧化物酶同工酶特性的关系[J]. 南京农业大学学报, 1993(1):123-126.
[40] 钟广炎, 聂华堂, 陈竹生. 叶片过氧化物酶活性用作柑桔矮化砧预选指标的初步研究[J]. 中国柑桔, 1989,18(1):19-20.
[41] 欧毅, 毛启才, 曹照春, 等. 不同砧木锦橙叶片过氧化物酶活性与植株生长结果的关系[J]. 云南农业大学学报, 1994,9(1):23-27.
[42] 吕斌, 陈学年, 李质怡, 等. 不同砧木先锋橙叶片POD活性与树体生长势的关系[J]. 西南农业学报, 1999,12(2):63-67.
[43] 张玉兰, 杨焕之. 枝叶解剖构造、过氧化物酶活性与山楂属种、株型生长势关系[J]. 内蒙古农牧学院学报, 1999, 20(1):46-51.
[44] Jones OP. Endogenous growth regulators and rootstock/scion interactions in apple and cherry trees[J]. International Symposium on Growth Regulators in Fruit Production, 1986, 179: 177-184.
[45] Lockard RQ Schneider GW. Stock and scion growth relationships and the dwarfing mechanism in apple[J]. Horticultural Reviews, 1981,3: 315-354.
[46] Atkinson CJ, Else M, Taylor L, et al. Root and stem hydraulic conductivity as determinants of growth potential in grafted tree of apple (Malus pumila Mill)[J]. Journal of Experimental Botany, 2003, 385: 1221-1229.
[47] Aloni B, Kami L, Deventurero Q et al. Physiological and biochemical changes at the rootstock‐scion interface in graft combinations between Cucurbita rootstocks and a melon scion[J]. Journal of Horticultural Science Biotechnology, 2008,83: 777-783.
[48] Aloni R, Cohen L, Kami H, et al. Hormonal signaling in rootstock-scion interactions[J]. Scientia Horticulturae. 2010, 127:119-126.
[49] 于迟, 张鹤, 李鸿莉, 等. 苹果矮化中间砧SH40激素含量及生长素转运蛋白基因pin1表达[J]. 中国农业大学学报, 2012,17(2):80-84.
[50] 汤常永. 梨‘中矮1号’矮生基因的筛选及 PcAHS克隆与表达分析[D]. 郑州: 中国农业科学院果树研究所, 2015.
[51] 王宏伟. 甜樱桃矮化砧木矮化机理研究[D]. 泰安: 山东农业大学, 2004.
[52] 宋晓敏. 苹果苗木质量评价与砧穗组合对幼树生长的影响[D]. 陕西杨凌: 西北农林科技大学, 2014.
[53] 张志华, 刘新彩, 刘彦红, 等. 核桃幼树内源激素与生长势的关系[J]. 林业科学, 2006,42(9):131-133.
[54] 田义柯. 苹果柱型基因作图及贝壳杉烯氧化酶基因cDNA片段克隆[D]. 杨凌:西北农林科技大学, 2004.
[55] 石琰景. 果树GA生物合成基因: 贝壳杉稀氧化酶和20—氧化酶的克隆及表达模式的初步研究[D]. 泰安: 山东农业大学, 2002.
[56] 姜志昂, 彭建营, 孙建设. 苹果砧木MdkS、MdKOA1基因克隆与表达分析[J]. 植物遗传资源学报, 2014,15(2): 362-368.
[57] 邓晓云. 苹果内根-贝壳杉烯合成酶基因的克隆及表达分析[J]. 华北农学报, 2013,28(2):46-51
[58] 程飞飞, 欧春青, 姜淑苓, 等. 梨内根-贝壳杉烯合酶基因克隆及表达分析[J]. 沈阳农业大学学报, 2011, 42(6): 677-682.
[59] 欧春青, 姜淑苓, 王斐, 等. 梨贝壳杉烯酸氧化酶基因PcKOA1的克隆与表达分析[J]. 园艺学报, 2013, 40(5): 849-858.
[60] 沙广利. 苹果无融合生殖矮化种质创新[D]. 泰安:山东农业大学, 2007.
[61] Kamboj J S, Blake P S, Quinlan J D, et al. Identification and quantitation by GC-MS of zeatin and zeatin riboside in xylem sap from rootstock and scion of grafted apple trees[J]. Plant Growth Regulation, 1999, 28: 199-205.
[62] 彭静, 彭福田, 魏绍冲, 等. 氮素形态对平邑甜茶IPT3表达与内源激素含量的影响[J]. 中国农业科学, 2008, 41(11):3716-3721.
[63] Li H L., Zhang H, Yu C, et al. Possible roles of auxin and zeatin for initiating the dwarfing effect of M9 used as apple rootstock or interstock[J]. Acta Physiologiae Plantarum, 2012, 34: 235-244.
[64] 邵开基, 牛自勉, 张忠仁, 等. 苹果砧木矮化程度与ABA含量的关系[J]. 园艺学报, 1987, 14(1): 7-11.
[65] 牛自勉, 梁德声. 苹果砧木脱落酸与树木生长规律的研究[J]. 北京林业大学学报, 1991,13(3):50-55.
[66] Jones OR. Effects of rootstocks and interstocks on the xylem sap composition in apple trees: Effects on nitrogen, phophorus and potassium content[J].Annals of Botany, 1971,35(4): 825-836.
[67] Simons, R.K. Graft-union characteristics as related to dwarfing in apple (Malus domestica Borkh.)[J]. Acta Horticulturae, 1986, 160:57-66.
[68] 张新忠,章德明,张建阁,等.矮砧及乔砧苹果树嫁接口的解剖观察[J].园艺学报,1995,22(2)：117-122.
[69] 冯轶, 张鹤, 李鸿丽, 吴婷, 王忆, 张新忠, 韩振海. 苹果矮化自根砧M9致矮机制的研究[J].园艺学报,2015,42(S1)：2578.
[70] 束怀瑞. 苹果学[M]. 北京：中国农业出版社, 2011:297.
[71] 陈长兰. 果树矮化砧和矮化中间砧的致矮机理研究[J]. 中国农学通报, 2000,16(3)：31-32.
[72] 沈隽. 华北农业大学果树文集[C]. 1977: 1-16.
[73] Gur A, Samish R. The role of auxin and auxin destruction in the vigour effect induced by various apple rootstocks[J]. Beitrage Biol. Pflanzen, 1968,45: 91-111.
[74] Martin G C, Stahly E A. Endogenous growth regulating factors in bark of EM IX and XVI apple trees [J]. Journal of the American Society for Horticultural Science, 1967, 91: 31-37