Response of Tobacco Planting Rhizosphere Soil Biological Activities to Biochar Combined with Compound-organic-bacteria-fertilizer

doi:10.11924/j.issn.1000-6850.casb18070097

Abstract

Abstract: The aim is to provide a basis for the reasonable application of biochar and compound-organicbacteria- fertilizer (COBF) in tobacco- planting soil. Based on the same amount of compound fertilizer application, six treatments were set up. Soil was sampled during the key tobacco growth stage (i.e. 30, 60, and 90 days after transplanted). The soil sample was analyzed for the effect of biochar and COBF on soil microorganisms’activities. The results showed that the sucrase activity in soil was significantly improved with T2 treatment in the whole growth period. The urease activity in soil under T2 and T3 treatments were significantly promoted attheearly growth stage (30d), and the urea seactivity was 0.74mg/(g·d)and0.75mg/(g·d), respectively. The microbial biomass carbon (SMBC, 810.09 mg/kg) and microbial biomass carbon entropy were improved under T2 treatment. The microbial biomass nitrogen (SMBN) and SMBN/TN were improved under T3 treatment during the whole tobacco growth period. The bacteria, fungi and actinomyce population were increased by COBF application. The highest population of bacteria and actinomyce appeared under T2 treatment during the whole tobacco growth period. Generally, in the central area of Henan Province, the application of COBBF and 60% COBBF combined with 40% biochar are the optimal practice to improve the soil microbial environment and fertility.

CLC Number:

S158

References

[1] 康雪莉,韩富根,王涵,等.植烟土壤养分和土壤酶活性的典型相关分析[J].河南农业科学,2013,42(7):52-56.
[2]王灿,王德建,孙瑞娟,等.长期不同施肥方式下土壤酶活性与肥力因素的相关性[J].生态环境,2008,17(2):688-692.
[3] 张翼,曹仕明,史俊,等.烟草土壤微生物与土壤酶活性分析[J].湖北农业科学,2012,51(19):4230-4232.
[4] 郭萍,文庭池,董玲玲,等.施肥对土壤养分含量、微生物数量和酶活性的影响[J].农业现代化研究,2011,32(3):363-366.
[5] 焦永吉,程功,马永健,等.烟草连作对土壤微生物多样性及酶活性的影响[J].土壤与作物,2014,3(2):56-62.
[6] Laddy J N, Amato M, Zhou L K, et al. Different effects of rotation plant residues and nitrogen fertilizer on microbial biomass and organic matter in Australian Alfiso[J]. Soil Biology ＆ Biochemistry, 1994, 26: 821-831.
[7] 饶霜,卢阳,黄飞,等.生物炭对土壤微生物的影响研究进展[J].生态与农村环境学报,2016,32(1):53-59.
[8] 施娴,刘艳红,王田涛,等.有机肥与烟草专用肥配施对植烟土壤微生物和土壤酶活性的动态变化[J].土壤通报,2017,48(5):1127-1131.
[9] 胡可,李华兴,卢维盛,等.生物有机肥对土壤微生物活性的影响[J].中国生态农业学报,2010,18(2):303-306.
[10] 陈心想,耿增超,王森,等.施用生物炭后塿土土壤微生物及酶活性变化特征[J].农业环境科学学报,2014,33(4):751-758.
[11] Dempster D N, Gleeson D B, Solaiman Z M, et al. Decreased soil microbial biomass and nitrogen mineralization with Eucalyptus biochar addition to coarse textured soil[J]. Plant and Soil, 2012, 354(1-2): 311-324.
[12] Ebhinmasto R, Chhonkar P K, Singh D, et al. Changes in soil biological and biochemical characteristics in a long-term field trial on a subtropical Inceptisol[J]. Soil Biology Biochemistry, 2006, 38(7): 1577-1582.
[13] 陈安强,付斌,鲁耀,等.有机物料输入稻田提高土壤微生物碳氮及可溶性有机碳氮[J].农业工程学报,2015,31(21):161-167.
[14] Friedel J K, Gabel D. Microbial biomass and microbial C: N ratio in bulk soil and buried bags for evaluating in situ net N mineralization in agricultural soils[J]. Journal of Plant Nutrient and Soil Science, 2001, 164: 673-679.
[15] 韩晓日,郑国砥,刘晓燕,等.有机肥与化肥配合施用土壤微生物量氮动态、来源和供氮特征[J].中国农业科学,2007,40(4):765-772.
[16] 关松荫.土壤酶及其研究法[M].北京:中国农业出版社,1986.
[17] 吴金水.土壤微生物生物量测定方法及其应用[M].北京:气象出版社,2006.
[18] 中国科学院南京土壤研究所微生物室编著.土壤微生物研究法[M].北京:科学出版社,1995.
[19] 刘海,杜如万,赵建,等.施肥对烤烟根际土壤酶活性及细菌群落结构的影响[J].烟草科技,2016,49(10):1-7.
[20] 周震峰,王建超,饶潇潇.添加生物炭对土壤酶活性的影响[J].江西农业学报,2015,27(6):110-112.
[21] 徐祥玉,孟贵星,袁家富,等.翻压绿肥对植烟土壤活性有机质和土壤酶的影响[J].中国烟草科学,2011,31(3):103-107.
[22] 王满,卫宣志,郭芳阳,等.动植物有机肥对烤烟根际土壤酶活性及微生物碳氮的影响[J].江西农业学报,2017,29(10):63-66.
[23] 张硕,张士荣,巩小红,等.有机肥混施对烤烟根际土壤酶活性及微生物碳氮含量的影响[J].青岛农业大学学报(自然科学版),2016,33(1):35-39.
[24] 李正,刘国顺,敬海霞,等.翻压绿肥对植烟土壤微生物量及酶活性的影响[J].草业学报,2011,20(3):225-232.
[25] 张弘,李影,张玉军,等.生物炭对植烟土壤氮素形态迁移及微生物量氮的影响[J].中国水土保持科学,2017,15(3):27-35.
[26] 赵军.生物质炭基氮肥对土壤微生物量碳氮、土壤酶及作物产量的影响研究[D].陕西杨凌:西北农林科技大学,2016.
[27] 梁利宝,李维宏,许剑敏.菌肥与有机无机肥配施对石灰性土壤不同形态氮素的影响[J].水土保持学报,2012,26(2):176-179.
[28] 李琰琰,刘国顺,向金友,等.氮营养水平对植烟土壤养分含量及土壤酶活性的影响[J].中国烟草学报,2011,12(2):39-43．
[29] 尹亚丽,王玉琴,鲍根生,等.退化高寒草甸土壤微生物及酶活性特征[J].应用生态学报,2017,27(12):3881-3890.
[30] 张冀.不同施肥措施对植烟土壤生化活性及烤烟产量品质的影响[D].武汉:华中农业大学,2010.
[31] 杜社妮,梁银丽,张成娥.施肥对西红柿土壤微生物和土壤呼吸的影响[J].干旱地区农业研究,2011,29(5):179-181.
[32] 冯伟,管涛,王晓宇,等.沼液与化肥配施对冬小麦根际土壤微生物数量和酶活性的影响[J].应用生态学报,2011,22(4):1007-1012.
[33] 李姣,刘国顺,高琴,等.不同生物有机肥与烟草专用复合肥配施对烤烟根际土壤微生物及土壤酶活性的影响[J].河南农业大学学报,2013,31(3):133-137.
[34] 韩富根,宋鹏飞,董祥洲,等.延边烟区不同土壤的根际土壤微生物生态效应研究[J].土壤,2010,42(1):33-38.
[35] 何虓,王强义,王明旭,等.生物有机肥与烟草专用复合肥配施对植烟土壤理化性质、微生物数量及酶活性的影响[J].耕作与栽培,2017,(1):22-24.
[36] Nanda S K, Das P K, Behera B. Effects of continuous manuring on microbial population ammonification and CO2 evolution in a rice soil[J]. Oryza, 1998, 25: 413-416.