The Control of Pepper Blight by Bacillus spp.: Research Progress

doi:10.11924/j.issn.1000-6850.casb19010137

Abstract

Abstract:

Pepper blight is a worldwide soil-borne disease, which occurs generally in pepper growing areas with more and more serious trend, and has caused huge economic loss. In order to find more efficient and environment-friendly biological control methods, and provide new ideas for disease control of pepper blight, the authors summarize the research about Bacillus spp. of pepper blight, and describe the following respects: strain source, screening method, strain identification method, culture fermentation and mechanism of disease control. The authors also discuss the way and main obstacles of Bacillus spp. application in practical production. Finally, the experimental condition, combination principles and cultivation methods of controlling pepper blight by Bacillus spp. are proposed.

Key words: Phytophthora capsici, Bacillus spp., biological control, microbial inoculants, soil microorganism

CLC Number:

S154.3

Ye Minshuo, Ma Yan, Huang Youjun. The Control of Pepper Blight by Bacillus spp.: Research Progress[J]. Chinese Agricultural Science Bulletin, 2020, 36(15): 123-129.

Figures/Tables 1

References 67

[1]	Cafefilho A C, Duniway J M, Davis R M . Effects of the frequency of furrow irrigation on root and fruit rots of squash caused by Phytophthora capsici[J]. Plant Disease, 1995,79(1):39-44. doi: 10.1094/PD-79-0039 URL
[2]	Hausbeck M K, Lamour K H . Phytophthora capsici on Vegetable Crops: Research Progress and Management Challenges[J]. Plant Disease, 2004,88(12):1292-1303. doi: 10.1094/PDIS.2004.88.12.1292 URL
[3]	吕和平, 郭满库, 陈雨天 , 等. 农业措施对辣椒疫病的生态控制效应[J]. 甘肃农业科技, 1998,36(3):44-46.
[4]	张维娜, 郑新光, 王伟丽 , 等. 辣椒品种抗疫病评价及不同砧木嫁接效果[J]. 北方园艺, 2013(16):1-5.
[5]	程子林, 张建树, 关铁炼 , 等. 采用嫁接方法防治青椒疫病的研究[J]. 天津农业科学, 1998,25(4):31-34.
[6]	张佑宏, 吕亮, 张舒 , 等. 咪唑菌酮?霜霉威盐酸盐对辣椒疫病的防治效果[J]. 湖北农业科学, 2017,56(4):661-662.
[7]	余宏达, 宋晓兵, 彭埃天 , 等. 新型复配药剂烯酰唑嘧菌对辣椒疫病的防治效果[J]. 长江蔬菜, 2017(4):78-80.
[8]	郭桂文, 郭成均, 徐恒涛 . 烯酰吗啉与氟啶胺混剂防治辣椒疫病[J]. 农药, 2016,55(8):600-601.
[9]	赖卫, 杨红, 刘崇政 , 等. 不同药剂对辣椒疫病的防治效果研究[J]. 长江蔬菜, 2016(14):61-63.
[10]	王虎生, 孙锦云 . 达科宁可湿性粉剂防治辣椒疫病田间药效试验[J]. 现代农业科技, 2016,45(3):148-151.
[11]	何允波, 唐丽萍, 张宝国 . 辣椒疫病菌的抗药性和新药剂的筛选研究[J]. 吉林农业科学, 2004,29(3):26-29,36.
[12]	Truong H T H, Kim J H , et al. Identification and development of molecular markers linked to phytophthora root ROT resistance in pepper[J]. European Journal of Plant Pathology, 2013,20(135):289-297.
[13]	Reifschneidbr F B, Café-filho A C . Factors affecting expression of resistance in pepper (Capsicum annuum) to blight caused by Phytophthora capsici in screening trials[J]. Plant Pathology, 1986,35(4):451-456. doi: 10.1111/ppa.1986.35.issue-4 URL
[14]	何延静, 刘海明, 胡洪波 , 等. 一株拮抗辣椒疫霉的假单胞菌的分离与鉴定[J]. 微生物学报, 2006,46(4):516-521.
[15]	韩长志 . 植物病原拮抗菌木霉属真菌的研究进展[J]. 江苏农业学报, 2016,32(4):946-952.
[16]	李松鹏, 崔琳琳, 程家森 , 等. 两株哈茨木霉菌株防治水稻纹枯病及促进水稻生长的潜力研究[J]. 植物病理学报, 2018,48(1):98-107.
[17]	王超, 郭坚华, 席运官 , 等. 拮抗细菌在植物病害生物防治中应用的研究进展[J]. 江苏农业科学, 2017,45(18):1-6.
[18]	徐刘平, 杨婷婷, 杨明明 , 等. 辣椒生境相关细菌分离及其对病原真菌的拮抗作用[J]. 江苏农业学报, 2008,24(6):896-900.
[19]	Yang R, Fan X, Cai X , et al. The inhibitory mechanisms by mixtures of two endophytic bacterial strains isolated from Ginkgo biloba against pepper phytophthora blight[J]. Biological Control, 2015,85(6):59-67. doi: 10.1016/j.biocontrol.2014.09.013 URL
[20]	Hu H Q, Li X S, Hong H . Characterization of an antimicrobial material from a newly isolated Bacillus amyloliquefaciens from mangrove for biocontrol of Capsicum bacterial wilt[J]. Biological Control, 2010,54(3):359-365. doi: 10.1016/j.biocontrol.2010.06.015 URL
[21]	Jiang Z Q, Guo Y H, Li S M , et al. Evaluation of biocontrol efficiency of different Bacillus preparations and field application methods against Phytophthora blight of bell pepper[J]. Biological Control, 2006,36(2):216-223. doi: 10.1016/j.biocontrol.2005.10.012 URL
[22]	Sang G K, Khan Z, Yong H J . Inhibitory effect of paenibacillus polymyxa GBR-462 on phytophthora capsici causing phytophthora blight in chili pepper[J]. Journal of Phytopathology, 2010,157(6):329-337. doi: 10.1111/jph.2009.157.issue-6 URL
[23]	Zhenzhen Z, Qiushuo W, Kaimei W , et al. Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components[J]. Bioresource Technology, 2010,20(1):292-297.
[24]	邱思鑫, 阮宏春, 宋美仙 , 等. 内生解淀粉芽孢杆菌TB2菌株活性物质诱导辣椒果抗疫病的生化机理[J]. 热带作物学报, 2010,31(10):1813-1820.
[25]	杨定祥, 林巧玲, 卢乃会 , 等. 拮抗辣椒疫霉菌海洋细菌菌株SH-27的筛选鉴定及其防病促生作用[J]. 微生物学通报, 2018,45(1):54-63. doi: 10.13344/j.microbiol.china.170221 URL
[26]	Liu H, Li S, Luo Y , et al. Biological control of Ralstonia wilt, Phytophthora blight, Meloidogyne root-knot on bell pepper by the combination of Bacillus subtilis AR12, Bacillus subtilis SM21 and Chryseobacterium spR89[J]. European Journal of Plant Pathology, 2014,139(1):107-116. doi: 10.1007/s10658-013-0369-2 URL
[27]	乔宏萍, 黄丽丽, 康振生 . 小麦内生细菌及其对根茎部主要病原真菌的抑制作用[J]. 应用生态学报, 2006,17(4):690-694.
[28]	Jäschke D, Dugassa-Gobena D, Karlovsky P , et al. Suppression of clubroot (Plasmodiophora brassicae) development in Arabidopsis thaliana by the endophytic fungus Acremonium alternatum[J]. Plant pathology, 2010,59(1):100-111. doi: 10.1111/ppa.2010.59.issue-1 URL
[29]	Paul N C, Ji S H, Deng J X , et al. Assemblages of endophytic bacteria in chili pepper (Capsicum annuum L.) and their antifungal activity against phytopathogens in vitro[J]. Plant Omics, 2013,6(6):441-448.
[30]	Lee K J, Kamala-Kannan S, Sub H S , et al. Biological control of Phytophthora blight in red pepper (Capsicum annuum L.) using Bacillus subtilis[J]. World journal of microbiology & biotechnology, 2008,24(7):1139-1145. doi: 10.1007/s11274-007-9585-2 URL
[31]	Byung-taek O, Hyun H, Kui-jae L , et al. Suppression of phytophthora blight on pepper (Capsicum annuum L.) by bacilli isolated from brackish environment[J]. Biocontrol Science & Technology, 2011,21(11):1297-1311.
[32]	Kim H S, Sang M K, Jeun Y C , et al. Sequential selection and efficacy of antagonistic rhizobacteria for controlling Phytophthora blight of pepper[J]. Crop Protection, 2008,27(3):436-443. doi: 10.1016/j.cropro.2007.07.013 URL
[33]	Park J W, Balaraju K, Kim J W , et al. Systemic resistance and growth promotion of chili pepper induced by an antibiotic producing Bacillus vallismortis strain BS07[J]. Biological Control, 2013,65(2):246-257. doi: 10.1016/j.biocontrol.2013.02.002 URL
[34]	Lee S H, Cho Y E, Park S H , et al. An antibiotic fusaricidin: a cyclic depsipeptide from Paenibacillus polymyxa E681 induces systemic resistance against Phytophthora blight of red-pepper[J]. Phytoparasitica, 2013,41(1):49-58. doi: 10.1007/s12600-012-0263-z URL
[35]	Naing K W, Anees M, Xuan H N , et al. Biocontrol of late blight disease (phytophthora capsici) of pepper and the plant growth promotion by paenibacillus ehimensis KWN38[J]. Journal of Phytopathology, 2014,162(6):367-376. doi: 10.1111/jph.12198 URL
[36]	Ahmed A S, Ezziyyani M, Sánchez C P , et al. Effect of chitin on biological control activity of Bacillus spp. and Trichoderma harzianum against root rot disease in pepper (Capsicum annuum) plants[J]. European Journal of Plant Pathology, 2003,109(6):633-637. doi: 10.1023/A:1024734216814 URL
[37]	汪涛, 迟元凯, 赵伟 , 等. 多粘类芽孢杆菌TC35的鉴定及对辣椒疫病的田间防效[J]. 安徽农业科学, 2018(4):138-139.
[38]	李悦, 余惠荣, 李蔚 , 等. 侧孢短芽孢杆菌B8对两种植物病原菌抗菌机制初步研究[J]. 中国植保导刊, 2015(3):12-16.
[39]	郝楠, 仝赞华, 邱德文 . 侧孢短芽孢杆菌的筛选及其对辣椒疫霉的室内防效测定[J]. 生物技术通报, 2017,33(09):160-165.
[40]	肖小露 . 枯草芽孢杆菌BS193对辣椒疫病的生防作用及其抗菌机制初探 Biological Control and Mechanism of Bacillus Subtilis BS193 against Pepper Phytophthora Blight(Phytophthora Capsici)[Z], 2017.
[41]	王巧 . 芽孢杆菌BS211生物活性及土壤处理对辣椒疫病的防控研究 The Study of Bioactivity of Bacillus BS211 and the Effect of Soil Treatment on Controlling Pepper Blight[Z], 2016.
[42]	肖淑芹, 薛春生, 周雪 , 等. 抗辣椒疫病枯草芽孢杆菌BS18液体发酵条件的研究[J]. 北方园艺, 2007(12):216-219.
[43]	Yi H S, Ahn Y R, Song G C , et al. Impact of a bacterial volatile 2,3-Butanediol onbacillus subtilisrhizosphere robustness[J]. Frontiers in Microbiology, 2016,7(12):557-568.
[44]	Castillo F H, Lirasaldivar R H, Gallegosmorales G , et al. Biocontrol of pepper wilt with three Bacillus species and its effect on growth and yield[J]. Revista internacional de contaminacion ambiental, 2016,18(83):49-55.
[45]	黄大野, 姚经武, 朱志刚 , 等. 枯草芽孢杆菌水分散粒剂防治辣椒疫病的效果及其对辣椒的促生作用[J]. 湖北农业科学, 2015,54(19):4737-4739.
[46]	Xu S, Kim B S . Evaluation of paenibacillus polymyxa strain SC09-21 for biocontrol of phytophthora blight and growth stimulation in pepper plants[J]. Tropical Plant Pathology, 2016,41(3):162-168. doi: 10.1007/s40858-016-0077-5 URL
[47]	Zhang M, Li J, Shen A , et al. Isolation and identification of bacillus amyloliquefaciens IBFCBF-1 with potential for biological control of phytophthora blight and growth promotion of pepper[J]. Journal of Phytopathology, 2016,164(11-12):1012-1021 doi: 10.1111/jph.12522 URL
[48]	李萌, 李丽, 张坦 , 等. 枯草芽孢杆菌固态发酵产脂肽培养基优化及脂肽的初步鉴定[J]. 食品工业科技, 2018,1(1):1-10.
[49]	郑双凤, 谭武贵, 丰来 , 等. 枯草芽孢杆菌-高产芽孢发酵工艺优化[J]. 南京农业大学学报, 2017,40(06):1031-1040.
[50]	李玉洋, 辛寒晓, 范学明 , 等. 菌糠固态发酵法培养多粘类芽孢杆菌的工艺[J]. 山东农业科学, 2017,49(12):119-125.
[51]	赵国群, 牛梦天, 卢士康 , 等. 梨渣固态发酵培养多粘类芽孢杆菌的工艺[J]. 农业工程学报, 2016(07):303-308.
[52]	Scher F M, Kloepper J W, Singleton C , et al. Colonization of soybean Roots by Pseudomonas and Serratia species: relationship to bacterial motility, chemotaxis, and Generation time[J]. Phytopathology, 1988,78(8):3574-3582.
[53]	何红, 邱思鑫, 蔡学清 , 等. 辣椒内生细菌BS-1和BS-2在植物体内的定殖及鉴定[J]. 微生物学报, 2004,44(1):13-18.
[54]	Yau J A, Dianez F, Marin F , et al. Screening and evaluation of potential biocontrol fungi and bacteria foliar endophytes against Phytophthora capsici and Phytophthora parasitica on pepper[J]. Journal of Food Agriculture & Environment, 2013,11(2):490-495.
[55]	Naing K W, Anees M, Sang J K , et al. Characterization of antifungal activity of Paenibacillus ehimensis KWN38 against soilborne phytopathogenic fungi belonging to various taxonomic groups[J]. Annals of Microbiology, 2014,64(1):55-63 doi: 10.1007/s13213-013-0632-y URL
[56]	Pieterse C M, Van Loon LC . NPR1: the spider in the web of induced resistance signaling pathways[J]. Current opinion in plant biology, 2004,7(4):456-464 doi: 10.1016/j.pbi.2004.05.006 URL
[57]	Yi H S, Yeo-rim A, Song G C , et al. Impact of a bacterial volatile 2,3-Butanediol onbacillus subtilisrhizosphere robustness:[J]. Frontiers in Microbiology, 2016,7(e0055712):557-568.
[58]	唐桢强, 黎柱, 何红 , 等. 菌株诱导的辣椒抗疫病生理生化特性研究[J]. 安徽农业科学, 2010,38(15):7924-7926
[59]	Burr T . Increased potato yields by treatment of seedpieces with specific strains of Pseudomonas fluorescens and Pputida[J]. Phytopathology, 1978,68(9):1377-1383. doi: 10.1094/Phyto-68-1377 URL
[60]	Abbasi M K, Sharif S, Kazmi M , et al. Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth,yield and nutrient uptake of plants[J]. Giornale Botanico Italiano, 2011,145(1):159-168.
[61]	Krey T, Caus M, Baum C , et al. Interactive effects of plant growth-promoting rhizobacteria and organic fertilization on P nutrition of Zea mays L. and Brassica napus L.[J]. Journal of Plant Nutrition and Soil Science = Zeitschrift FUER Pflanzenernaehrung UND Bodenkunde, 2011,174(4):602-613.
[62]	Egamberdiyeva D . The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils[J]. Applied Soil Ecology, 2007,36(2):184-189. doi: 10.1016/j.apsoil.2007.02.005 URL
[63]	Hafeez F Y, Yasmin S, Ariani D , et al. Plant growth-promoting bacteria as biofertilizer[J]. Agronomy for Sustainable Development, 2006,26(2):143-150. doi: 10.1051/agro:2006007 URL
[64]	Suneja P, Dudeja S S, Narula N . Development of multiple co-inoculants of different biofertilizers and their interaction with plants[J]. Archives of Agronomy & Soil Science, 2007,53(2):221-230.
[65]	Lutfi P, Metin T, Fikrettin S , et al. Floral and foliar application of plant growth promoting rhizobacteria (pgpr) to apples increases yield, growth, and nutrient element contents of leaves[J]. Journal of Sustainable Agriculture, 2007,30(4):145-155. doi: 10.1300/J064v30n04_11 URL
[66]	黄大野, 姚经武, 朱志刚 , 等. 枯草芽孢杆菌水分散粒剂防治辣椒疫病的效果及其对辣椒的促生作用[J]. 湖北农业科学, 2015,54(19):4737-4739.
[67]	杨定祥, 林巧玲, 卢乃会 , 等. 拮抗辣椒疫霉菌海洋细菌菌株SH-27的筛选鉴定及其防病促生作用[J]. 微生物学通报, 2018,45(1):54-63. doi: 10.13344/j.microbiol.china.170221 URL

名称	菌种来源	试验方式	被证实有效代谢产物
特基拉芽孢杆菌^[29](CNU082075)	辣椒根部	平板对峙	/
枯草芽孢杆菌R13、R33^[30]	辣椒根际	平板对峙	水解酶、氰化氢
路西法芽孢杆菌KJ2C12、KJ1R5、KJ9C8^[32]	田间土壤	根部侵染、种子实验、苗床试验、田间试验	/
解淀粉芽孢杆菌Fy11、Zy44^[19]	银杏叶片	盆栽试验	粗脂肽
芽孢杆菌分离株SB10^[31]	盐碱土壤	平板对峙、盆栽试验	脂肽芬荠素、伊枯草菌素和表面活性素
死谷芽孢杆菌BS07^[33]	辣椒根际	平板对峙、盆栽试验、田间试验	水杨酸
多粘类芽孢杆菌E681^[34]	微生物资源库	提取物平板拮抗、盆栽试验	镰孢菌素
爱媛类芽孢杆菌KWN38^[35]	田间土壤	盆栽试验	β-1,3葡聚糖酶
解淀粉芽孢杆菌Bg-C31^[20]	红海榄植株	平板对峙、盆栽试验、田间试验	融合蛋白
枯草芽孢杆菌HS93^[36]	辣椒根部	平板对峙、田间试验	几丁质酶
地衣芽孢杆菌LS234、LS523、LS674^[36]	辣椒根部	平板对峙、田间试验	几丁质酶
芽孢杆菌分离株BB11^[21]	生姜根际	田间试验	/
芽孢杆菌分离株FH17^[21]	甜椒植株	田间试验	/
多粘类芽孢杆菌GBR-462^[22]	人参根际	平板对峙、盆栽试验	/
死谷芽孢杆菌ZZ185^[23]	冬青茎部	提取物平板拮抗	杆菌霉素D
解淀粉芽孢杆菌TB2^[24]	烟草茎部	提取物平板拮抗	/
多粘类芽孢杆菌TC35^[37]	田间土壤	平板对峙、田间试验	/
侧孢短芽孢杆菌B8^[38]	烟草根际	平板对峙	几丁质酶
侧孢短芽孢杆菌A60^[39]	田间土壤	平板对峙、盆栽试验	/
枯草芽孢杆菌BS193^[40]	实验室保存	平板对峙、盆栽试验	丰原菌素、伊枯草菌素和表面活性素
枯草芽孢杆菌BS211^[41]	西瓜植株	平板对峙、盆栽试验	/
枯草芽孢杆菌BS18^[42]	辣椒根际	盆栽试验、田间试验	/
枯草芽孢杆菌168、BSIP1174、BSIP1171^[43]	辣椒根际	盆栽试验	2,3-丁二醇
解淀粉芽孢杆菌B1、地衣芽孢杆菌B3、枯草芽孢杆菌B13^[44]	辣椒根际	平板对峙、幼苗培养	2,3-丁二醇
枯草芽孢杆菌^[45]	生物公司生产	盆栽试验	/
解淀粉芽孢杆菌SH-27^[25]	石珊瑚	平板对峙、盆栽试验	/
枯草芽孢杆菌AR12^[26]	大白菜根际	平板对峙、盆栽试验、田间试验	/
枯草芽孢杆菌SM21^[26]	松树根际	平板对峙、盆栽试验、田间试验	/
多粘类芽孢杆菌SC09-21^[46]	田间土壤	平板对峙、盆栽试验	氨、纤维素酶、吲哚-3-乙酸、蛋白酶以及增溶磷酸钙
解淀粉芽孢杆菌IBFCBF1^[47]	辣椒根际	平板对峙、盆栽试验	/

名称	菌种来源	试验方式	被证实有效代谢产物
特基拉芽孢杆菌^[29](CNU082075)	辣椒根部	平板对峙	/
枯草芽孢杆菌R13、R33^[30]	辣椒根际	平板对峙	水解酶、氰化氢
路西法芽孢杆菌KJ2C12、KJ1R5、KJ9C8^[32]	田间土壤	根部侵染、种子实验、苗床试验、田间试验	/
解淀粉芽孢杆菌Fy11、Zy44^[19]	银杏叶片	盆栽试验	粗脂肽
芽孢杆菌分离株SB10^[31]	盐碱土壤	平板对峙、盆栽试验	脂肽芬荠素、伊枯草菌素和表面活性素
死谷芽孢杆菌BS07^[33]	辣椒根际	平板对峙、盆栽试验、田间试验	水杨酸
多粘类芽孢杆菌E681^[34]	微生物资源库	提取物平板拮抗、盆栽试验	镰孢菌素
爱媛类芽孢杆菌KWN38^[35]	田间土壤	盆栽试验	β-1,3葡聚糖酶
解淀粉芽孢杆菌Bg-C31^[20]	红海榄植株	平板对峙、盆栽试验、田间试验	融合蛋白
枯草芽孢杆菌HS93^[36]	辣椒根部	平板对峙、田间试验	几丁质酶
地衣芽孢杆菌LS234、LS523、LS674^[36]	辣椒根部	平板对峙、田间试验	几丁质酶
芽孢杆菌分离株BB11^[21]	生姜根际	田间试验	/
芽孢杆菌分离株FH17^[21]	甜椒植株	田间试验	/
多粘类芽孢杆菌GBR-462^[22]	人参根际	平板对峙、盆栽试验	/
死谷芽孢杆菌ZZ185^[23]	冬青茎部	提取物平板拮抗	杆菌霉素D
解淀粉芽孢杆菌TB2^[24]	烟草茎部	提取物平板拮抗	/
多粘类芽孢杆菌TC35^[37]	田间土壤	平板对峙、田间试验	/
侧孢短芽孢杆菌B8^[38]	烟草根际	平板对峙	几丁质酶
侧孢短芽孢杆菌A60^[39]	田间土壤	平板对峙、盆栽试验	/
枯草芽孢杆菌BS193^[40]	实验室保存	平板对峙、盆栽试验	丰原菌素、伊枯草菌素和表面活性素
枯草芽孢杆菌BS211^[41]	西瓜植株	平板对峙、盆栽试验	/
枯草芽孢杆菌BS18^[42]	辣椒根际	盆栽试验、田间试验	/
枯草芽孢杆菌168、BSIP1174、BSIP1171^[43]	辣椒根际	盆栽试验	2,3-丁二醇
解淀粉芽孢杆菌B1、地衣芽孢杆菌B3、枯草芽孢杆菌B13^[44]	辣椒根际	平板对峙、幼苗培养	2,3-丁二醇
枯草芽孢杆菌^[45]	生物公司生产	盆栽试验	/
解淀粉芽孢杆菌SH-27^[25]	石珊瑚	平板对峙、盆栽试验	/
枯草芽孢杆菌AR12^[26]	大白菜根际	平板对峙、盆栽试验、田间试验	/
枯草芽孢杆菌SM21^[26]	松树根际	平板对峙、盆栽试验、田间试验	/
多粘类芽孢杆菌SC09-21^[46]	田间土壤	平板对峙、盆栽试验	氨、纤维素酶、吲哚-3-乙酸、蛋白酶以及增溶磷酸钙
解淀粉芽孢杆菌IBFCBF1^[47]	辣椒根际	平板对峙、盆栽试验	/