Overexpression and Silencing of Z257 SnoRNA Gene Under Drought Stress: Effects on Drought Tolerance and Phenotype of Rice

doi:10.11924/j.issn.1000-6850.casb20190700474

Abstract

Abstract:

To analyze the effects of overexpression and gene silencing of SnoRNA Z257 on drought tolerance, agronomic trait and quality trait of rice, using transgenic rice lines and wild type control Nipponbare as research materials, the action mode of SnoRNA Z257 was studied by drought at seedling stage and heading stage. The results showed that the drought tolerance of the transgenic rice line GB Nipponbare (overexpressing SnoRNA Z257) was higher than that of wild type control Nipponbare and the transgenic rice line GM Nipponbare (silencing SnoRNA Z257). Therefore, the SnoRNA Z257gene was related to drought tolerance of rice. After drought stress at seedling stage, the root system of GB Nipponbare was stronger than that of wild Nipponbare, and that of CM Nipponbare was the weakest. After drought stress at heading stage, the agronomic traits of GB Nipponbare were better than that of wild type control Nipponbare, and that of CM Nipponbare was the worst. The quality traits of GB Nipponbare were better than those of wild type control Nipponbare and CM Nipponbare (silencing SnoRNA Z257). The quality traits of CM Nipponbare (silencing SnoRNA Z257) were affected mostly under drought stress, and the quality traits were the poorest. SnoRNA Z257 is highly correlated to the rice drought tolerance, which can reduce the effects of drought stress on rice root, agronomic and quality traits, and can be used as a candidate gene for drought tolerance.

Key words: drought stress, SnoRNA, overexpression and silencing of gene, drought tolerance, agronomic traits and quality traits

CLC Number:

Ma Tingchen, Xia Jiafa, Wang Yuanlei, Zhou Kunneng, Yun Peng, Li Zefu. Overexpression and Silencing of Z257 SnoRNA Gene Under Drought Stress: Effects on Drought Tolerance and Phenotype of Rice[J]. Chinese Agricultural Science Bulletin, 2020, 36(23): 1-5.

Figures/Tables 4

References 25

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品系	处理第2天	处理第3天	处理第4天	处理第5天	处理第6天
GB日本晴	6	10	21	35	41
CM日本晴	13	22	42	64	75
日本晴	10	18	33	49	62

品系	处理第2天	处理第3天	处理第4天	处理第5天	处理第6天
GB日本晴	6	10	21	35	41
CM日本晴	13	22	42	64	75
日本晴	10	18	33	49	62

品系	根体积/mL	根粗/cm	最长根长/cm	根总长度/cm	根数量/根	侧根长/cm
GB日本晴	0.431 a	0.321 a	17.124 a	58.774 a	8.425 a	23.557 a
CM日本晴	0.284 c	0.241 c	12.655 c	44.124 c	6.224 c	15.621 c
日本晴	0.358 b	0.284 b	16.234 b	53.156 b	7.652 b	20.355 b

品系	根体积/mL	根粗/cm	最长根长/cm	根总长度/cm	根数量/根	侧根长/cm
GB日本晴	0.431 a	0.321 a	17.124 a	58.774 a	8.425 a	23.557 a
CM日本晴	0.284 c	0.241 c	12.655 c	44.124 c	6.224 c	15.621 c
日本晴	0.358 b	0.284 b	16.234 b	53.156 b	7.652 b	20.355 b

品系	株高/cm	穗数/个	穗长/cm	结实率/%	单株谷重/g	千粒重/g
GB日本晴	88.1 a	25 a	26.5 a	85.7 a	55.4 a	24.6 a
CM日本晴	86.3 a	22 b	22.2 c	81.5 b	48.1 c	21.3 b
日本晴	87.5 a	24 a	23.4 b	84.2 a	52.6 b	23.7 a